ZNF424, a novel human KRAB/C2H2 zinc finger protein, suppresses NFAT and p21 pathway

Wang, Yue Qun; Zhou, Jun; Ye, Xiang Li; Wan, Yong; Li, Yong Qing; Mo, Xiao Yan; Wu, Yuan; Yan, Yan; Luo, Na; Wang, Ze Qun; Fan, Xiong Wei; Deng, Yun; Wu, Xiu Shan

doi:10.5483/bmbrep.2010.43.3.212

Cited by 5 publications

(9 citation statements)

References 24 publications

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“…Northern blotting was performed using a [α- 32 P]dCTP-labeled cDNA probe, followed by hybridization with a glyceraldehyde-3-phosphate dehydrogenase ( gapdh ) cDNA probe, as described previously (22).…”

Section: Methodsmentioning

confidence: 99%

Egr-1 regulates the transcription of the BRCA1 gene by etoposide

Shin

Kim²,

Lee

2013

BMB Reports

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The breast cancer susceptibility gene BRCA1 encodes a nuclear protein, which functions as a tumor suppressor and is involved in gene transcription and DNA repair processes. Many families with inherited breast and ovarian cancers have mutations in the BRCA1 gene. However, only a few studies have reported on the mechanism underlying the regulation of BRCA1 expression in humans. In this study, we investigated the transcriptional regulation of BRCA1 in HeLa cells treated with etoposide. We found that three Egr-1-binding sequences (EBSs) were located at −1031, −1005, and −385 within the enhancer region of the BRCA1 gene. Forced expression of Egr-1 stimulated the BRCA1 promoter activity. EMSA data showed that Egr-1 bound directly to the EBS within the BRCA1 gene. Knockdown of Egr-1 through the expression of a small hairpin RNA (shRNA) attenuated etoposide-induced BRCA1 promoter activity. We conclude that Egr-1 targets the BRCA1 gene in HeLa cells exposed to etoposide. [BMB Reports 2013; 46(2): 92-96]

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Section: Methodsmentioning

confidence: 99%

Egr-1 regulates the transcription of the BRCA1 gene by etoposide

Shin

Kim²,

Lee

2013

BMB Reports

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“…Although more than one hundred members of the KRAB/C2H2 zinc-finger protein family have been described, little is known of their biological function (12). Increasingly, studies have implicated ZNF proteins in embryonic development and disease (1,(13)(14)(15)(16)(17)(18). For example, the Kruppel-class C2H2 zinc-finger protein ZNF382 has been shown to play a role in myogenesis (13, Wu, unpublished) and in tumor suppression through heterochromatin-mediated silencing (17).…”

Section: Discussionmentioning

confidence: 99%

“…The consensus sequence of the conserved Cys2/His2 type zinc finger motifs was used to search human EST database through a combined BLAST search as previously described (18). PCR was performed with one pair of primers P1/P2 (Supplement Table 2) on a PCR SPRINT reactor (Thermo Hybaid, USA).…”

Section: Full-length Znf425 Cdna Cloning and Bioinformatics Analysismentioning

confidence: 99%

“…BLASTn program was used to search a human EST database to identify the cytological locus of genes and to look for exons and introns. The homologs of ZNF425 were found with BLASTp, and the sequence alignment and phylogenetic tree analysis were performed with MegAlign program (BGI Life Tech, China) Cell culture and subcellular localization analysis COS-7 cells were maintained and passaged in DMEM according to standard methods (Dulbeccos modified Eagles medium, Gibco-BRL) and were transfected with pEGFP-N1-ZNF425 using LipofectAMINE (Invitrogen, USA) according to the method described previously (18). 48 hours after transfection, cells were fixed with 4% paraformaldehyde for 15 min and nuclear fractions were stained with 4', 6'-diamidino-2-phenylindole hydrochloride (DAPI).…”

Section: Full-length Znf425 Cdna Cloning and Bioinformatics Analysismentioning

confidence: 99%

“…To understand the potential transcriptional regulatory activity of ZNF425, the full length pCMV-BD-ZNF425 and truncated fragments were transfected into COS-7 cells along with L8G5-Luciferase and pLexA-VP16 using LipofectAMINE as described previously (18). To examine the effects of ZNF425 on the transcriptional activity of SRE, AP-1 and SRF, COS-7 cells were also co-transfected with the expression plasmids pCMV-Tag2B-ZNF425, pSRE-Luc, pAP-1-Luc, pCMV-Tag2B or the pSRF-Luc vector.…”

Section: Transcriptional Reporter Gene Assaysmentioning

confidence: 99%

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A novel human KRAB-related zinc finger gene ZNF425 inhibits mitogen-activated protein kinase signaling pathway

Wang

Ye²,

Zhou³

et al. 2011

BMB Reports

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Zinc finger (ZNF) proteins play a critical role in cell growth, proliferation, apoptosis, and intracellular signal transduction. In this paper, we cloned and characterized a novel human KRAB-related zinc finger gene, ZNF425, which encodes a protein of 752 amino acids. ZNF425 is strongly expressed in the three month old human embryos and then is almost undetectable in six month old embryos and in adult tissues. An EGFP-ZNF425 fusion protein can be found in both the nucleus and the cytoplasm.

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Identifying Evolutionary Origins of Repeat Domains in Protein Families

Aluru

Singh

2020

Proceedings of the 11th ACM International Conference on Bioinformatics, Computational Biology and Health Informatics

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ZNF424, a novel human KRAB/C2H2 zinc finger protein, suppresses NFAT and p21 pathway

Cited by 5 publications

References 24 publications

Egr-1 regulates the transcription of the BRCA1 gene by etoposide

Egr-1 regulates the transcription of the BRCA1 gene by etoposide

A novel human KRAB-related zinc finger gene ZNF425 inhibits mitogen-activated protein kinase signaling pathway

Identifying Evolutionary Origins of Repeat Domains in Protein Families

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