ZnO nanoparticle/nanorod-based label-free electrochemical immunoassay for rapid detection of MMP-9 biomarker

Shabani, Ehsan; Abdekhodaie, Mohammad J.; Mousavi, Seyyed Abbas; Taghipour, Fariborz

doi:10.1016/j.bej.2020.107772

Cited by 30 publications

(9 citation statements)

References 39 publications

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“…After the antigen and antibody are combined, the electrode surface's electric double layer or charge transfer resistance changes, which can be reflected in the EIS. Antigens and antibodies can be immobilized on the electrode surface, such as indium tin oxide glass electrodes, 169 gold screen-printed electrodes, zinc oxide nanorod electrodes, 122 and magnetic glass carbon electrodes. 121 When VEGF antibodies (Abs) were modified on the surface of MNPs@Au, a magnetic complex (MNPs@Au-Ab) was constructed and immobilized on a magnetic glassy carbon electrode, and then VEGF was captured (Fig.…”

Section: Electrochemistrymentioning

confidence: 99%

Recent progress of biosensors for the detection of lung cancer markers

Chen

Weng

et al. 2023

J. Mater. Chem. B

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Section: Electrochemistrymentioning

confidence: 99%

Recent progress of biosensors for the detection of lung cancer markers

Chen

Weng

et al. 2023

J. Mater. Chem. B

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“…Shabani et al (2020) reported MMP-9 detection by the electrochemical method using zinc oxide (ZnO) nanoparticles and a ZnO nanorod-modified substrate [ 75 ]. An Au-coated substrate was modified using ZnO nanoparticles, and its concentration was optimized using the CV method.…”

Section: Electrochemical Detection Of Biomoleculesmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells

2020

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Electrochemical sensors are considered an auspicious tool to detect biomolecules (e.g., DNA, proteins, and lipids), which are valuable sources for the early diagnosis of diseases and disorders. Advances in electrochemical sensing platforms have enabled the development of a new type of biosensor, enabling label-free, non-destructive detection of viability, function, and the genetic signature of whole cells. Numerous studies have attempted to enhance both the sensitivity and selectivity of electrochemical sensors, which are the most critical parameters for assessing sensor performance. Various nanomaterials, including metal nanoparticles, carbon nanotubes, graphene and its derivatives, and metal oxide nanoparticles, have been used to improve the electrical conductivity and electrocatalytic properties of working electrodes, increasing sensor sensitivity. Further modifications have been implemented to advance sensor platform selectivity and biocompatibility using biomaterials such as antibodies, aptamers, extracellular matrix (ECM) proteins, and peptide composites. This paper summarizes recent electrochemical sensors designed to detect target biomolecules and animal cells (cancer cells and stem cells). We hope that this review will inspire researchers to increase their efforts to accelerate biosensor progress—enabling a prosperous future in regenerative medicine and the biomedical industry.

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“…ZnO has been well documented regarding multifunctional wide and direct band gap (as per E-K relationship), with n type semiconductors having excellent nanoscale sizedependent variable optical properties, which is of great interest within nanoparticle (NP)-based drug delivery, biomolecular selectivity and sensitivity, bio-imaging, and biomedical research. [1][2][3][4][5] Due to large direct band gap and excellent optical transparency, ZnO is used in many optoelectronic diagnostic devices. [6] ZnO nanorods (ZnO NRs) can perform protein microarray applications and highly sensitive biomolecular fluorescence detection.…”

Section: Introductionmentioning

confidence: 99%

Exciton–tryptophan coupling pulse behaviour along with corona formation, binding analysis, and interaction study of ZnO nanorod–serum albumin protein bioconjugate

Bhunia¹,

Jha

Saha

2022

Luminescence

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The bioconjugate of bovine serum albumin (BSA) and zinc oxide nanorods (ZnO NRs) is investigated to explore the behaviour of the tryptophan (Trp)-exciton coupling and corona formation. The pulse like nature of the coupled system between Trp of BSA and exciton of ZnO NRs has been observed after analysis of the optical parameters such as refractive index, susceptibility, and optical dielectric constant. The time constant for tryptophan, exciton surface binding (t 1 ) and reorganization (t 2 ) are found to be (t 1 ) 8 min, 7 min and (t 2 ) 150 min, 114.5 min, respectively. The close proximity binding of BSA with ZnO NRs via tryptophan as well as exciton is responsible for bioconjugate formation. The aggregated structure of BSA is observed from smallangle X-ray scattering study in interaction with ZnO NRs. The change in secondary structure and tertiary deformation of the serum protein have been studied from Fourier transform infrared and emission quenching analyses. The number of binding sites (n) signified to the enhancement of the cooperative binding. The binding has been found to be endothermic and favoured using unfavourable positive enthalpy with a favourable entropy change from the result of the isothermal titration calorimetry.

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ZnO nanoparticle/nanorod-based label-free electrochemical immunoassay for rapid detection of MMP-9 biomarker

Cited by 30 publications

References 39 publications

Recent progress of biosensors for the detection of lung cancer markers

Recent progress of biosensors for the detection of lung cancer markers

Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells

Exciton–tryptophan coupling pulse behaviour along with corona formation, binding analysis, and interaction study of ZnO nanorod–serum albumin protein bioconjugate

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