Zoledronic acid and radiation: toxicity, synergy or radiosensitization?

Alcaraz, M.; Olivares, Á.; Armero, D; Alcaraz-Saura, Miguel; Achel, Daniel Gyingiri

doi:10.1007/s12094-012-0917-9

Cited by 10 publications

(16 citation statements)

References 20 publications

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“…It has been shown that activated cells produce more formazan than resting cells; therefore, it is possible to measure cell activity or enzyme activities ( 4 ). The cytokinesis-block micronucleus assay (MNCB) on human lymphocytes which is based on the increase in the frequency of appearance of MN is the most commonly used assay for determining the mutagenic capacity of a chemical or physical genotoxic agents ( 5 , 7 , 9 ). Both assays have been used extensively to evaluate the genotoxic and cytotoxic effects of chemical substances and physical agents in toxicological tests.…”

Section: Discussionmentioning

confidence: 99%

“…The genotoxicity test has been described in cultured human lymphocytes exposed to radiation and is considered the ideal assay for measuring chromosomal damage and quantify the genotoxic capacity of such substances ( 5 , 7 , 9 ).…”

Section: Discussionmentioning

confidence: 99%

“…Ionizing radiations (IR) causes the generation of a high level of hydroxyl radicals in vivo , through the hemolytic cleavage of body water or of endogenous hydrogen peroxide (formed by reduction of the superoxide anion) by two mechanisms: the Haber-Weiss and Fenton models. The hydroxyl radical is the most cytotoxic of all these so far described, with an estimated half-life of 10-9 s. the high reactivity of this radical implies immediate reaction at the place where it is generated ( 5 , 7 ). Thus, when hydroxyl radical generation is massive, as occurs during X-irradiation, the genotoxic and cytotoxic effect increases through the interaction of these radicals with cell phospholipid structures, inducing peroxidation processes and the generation of lipoperoxy radicals, which may be regarded as a delayed reaction by ionizing radiation ( 7 , 9 ).…”

Section: Discussionmentioning

confidence: 99%

“…The MTT assay has been extensively used to assess the cytotoxicity of dental materials and indicates cell viability based on mitochondrial dehydrogenase activity. It determines the degree to which cell proliferation is inhibited ( 4 ) and, in radiobiology, the cell survival rate against toxic agents ( 5 , 6 ).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Toxicity of a dental adhesive compared with ionizing radiation and zoledronic acid

Baños¹,

Olivares²,

Achel³

et al. 2015

Med Oral

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BackgroundTo determine the toxicity of aqueous dilutions of a universal self-priming dental adhesive (DA) and comparing these with those elicited by exposure to ionizing radiation (IR), Zoledronic acid (Z) treatment and the synergic effects of the combined treatment with IR+Z.Material and MethodsThe genotoxic effect of DA was determined by the increase in the frequency of micronuclei in cytokinesis-blocked in cultured human lymphocytes before and after exposure to 2Gy of X-rays. The cytotoxic effect was studied by using the MTT cell viability test in normal prostate cell lines (PNT2) after exposure to different X-ray doses (0Gy-20Gy). The cell lines divided into different groups and treated with different test substances: DA in presence of O2, DA in absence of O2, Z-treated and control.ResultsAn in vitro dose-dependent and time-dependent cytotoxic effect of DA, Z and IR on PNT2 cells (p>0.001) was demonstrated. DA without-O2, following the recommendations of manufacturers, had a more pronounced effect of increasing cell death than DA with-O2 (p<0.001). In the genotoxicity assay, DA at 25% of its original concentration significantly increased chromosome damage (p<0.001). The samples studied were found to be toxic, and the samples photo-polymerized in absence of O2 showed a bigger cytotoxic effect comparable to the additive toxic effect showed by the combined treatment of IR+Z.ConclusionsAdditional effort should be carried out to develop adhesives, which would reduce the release of hazardous substances; since toxic effects are similar to that reported by other agents whose clinical use is controlled by the health authorities. Key words: Micronucleus, toxicity, dental adhesive, zolendronic acid, radiation effects.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Toxicity of a dental adhesive compared with ionizing radiation and zoledronic acid

Baños¹,

Olivares²,

Achel³

et al. 2015

Med Oral

Self Cite

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show abstract

“…This study found that 100 lM ZOL at 72 h without radiation was the concentration with the greatest effect on OSCC cell viability; this raises various issues, for example, the question of when to administer ZOL. Alcaraz et al (37) found that the effect of ZOL was always greater after irradiation. Regarding genotoxic effects, only ZOL administration after irradiation (IR plus ZOL) increased chromosome damage and the sensibilization factor (35.7%).…”

Section: J Oral Pathol Medmentioning

confidence: 96%