Zone Electrophoretic Studies of Serum Alkaline Phosphatase*†

Rosenberg, I. N.

doi:10.1172/jci103841

Cited by 62 publications

(11 citation statements)

References 19 publications

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“…The views of previous workers (Rosenberg, 1959;Keiding, 1959) regarding the existence in the serum of several alkaline phosphatase fractions with different electrophoretic mobilities were supported by the present study. The peak of alkaline phosphatase activity was normally found in the alpha 2 globulins, and the beta fraction had somewhat more than half the alpha 2 activity.…”

Section: Discussionsupporting

confidence: 79%

“…The suggestion (Keiding, 1959) that beta globulin phosphatase has a special relationship to increased bone phosphatase activity, and that alpha 1 phosphatase has a connexion with liver phosphatase (Rosenberg, 1959), is supported by the present study, though increased enzyme activity in these fractions is not specific for bone or hepatobiliary disorders respectively. Rosenberg (1959) has postulated an alternative interpretation of the findings.…”

Section: Discussionsupporting

confidence: 71%

“…The proportion of phosphatase activity in each fraction has been found to vary in different diseases of bone and liver, and this has suggested the possibility that enzyme moving with a certain fraction may be derived from a particular tissue, or at least has some connexion with it. This relationship has been postulated for beta globulin phosphatase and bone (Keiding, 1959;Kowlessar et al, 1959) and for alpha 1 globulin phosphatase and liver (Rosenberg, 1959;Keiding, 1959) on the basis of increased enzyme activity found in those fractions in some cases of bone and liver disease respectively. Increased alpha 2 globulin phosphatase activity has been found in both liver and bone disease.…”

mentioning

confidence: 99%

“…Paper (Baker and Pellegrino, 1954) and starch (Rosenberg, 1959;Keiding, 1959; Kowlessar, Pert, Haeffner, and Sleisenger, 1959) media have been used for the separation, and in normal serum peak enzyme activity is in the alpha 2 fraction. The proportion of phosphatase activity in each fraction has been found to vary in different diseases of bone and liver, and this has suggested the possibility that enzyme moving with a certain fraction may be derived from a particular tissue, or at least has some connexion with it.…”

mentioning

confidence: 99%

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Distribution of alkaline phosphatase in serum protein fractions

Korner

1962

Journal of Clinical Pathology

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sYNopsis A technique for measuring the alkaline phosphatase activity of serum protein fractions separated by electrophoresis on cellulose acetate strips is described.Alkaline phosphatase activity in alpha 2, alpha 1, and beta globulins and in albumin is present in varying proportions in normal and pathological sera. In normal sera peak activity is in the alpha 2 globulins and beta globulins have a little more than half the alpha 2 activity. Albumin activity is often more than 10 % of the total, but alpha 1 and gamma globulin activity is less than this.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionsupporting

confidence: 71%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Distribution of alkaline phosphatase in serum protein fractions

Korner

1962

Journal of Clinical Pathology

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“…Rosenberg [50] and Keiding [32], using starch granules as supporting medium during electrophoresis, found in general that increased alkaline phosphatase activity associated with liver dis orders had a greater mobility than that associated with bone diseases. It is probable that these zones of activity correspond to the A and B zones of the starch gel system.…”

Section: Tissues Of Originmentioning

confidence: 99%

Genetically Determined Variants of Serum Alkaline Phosphatase: A Review

Robinson¹,

Goldsmith²

1967

Vox Sanguinis

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Separation of alkaline phosphatase enzymes in human serum using gel-filtration (Sephadex G-200) techniques

Dunne

Fennelly

McGeeney

1967

Cancer

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With the aid of gel filtration techniques (Sephadex G‐200) the alkaline phosphatase (Bessey‐Lowry‐Brock) distribution patterns have been studied in the serum of control human subjects and of those with conditions known to affect total enzyme levels. Protein was scanned at 254 and 280 mμ and the 19S, 7S, and 4S peaks thus obtained were used as points of reference. The characteristic control serum revealed one sharp peak of enzyme activity in the 7S protein region. A similar but sharper peak was found in osteomalacia and Pagets' disease. In contrast, in malignant bone conditions 10.4%, in metastatic malignant liver disease 31.36% and in nonmalignant liver disease 12.7% of total enzyme moved with the 19S proteins. The patterns found in various conditions are reproducible, and that of metastatic malignant liver disease is particularly characteristic. The significance of the macromolecular‐moving enzyme is discussed and is being investigated.

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Zone Electrophoretic Studies of Serum Alkaline Phosphatase*†

Cited by 62 publications

References 19 publications

Distribution of alkaline phosphatase in serum protein fractions

Distribution of alkaline phosphatase in serum protein fractions

Genetically Determined Variants of Serum Alkaline Phosphatase: A Review

Separation of alkaline phosphatase enzymes in human serum using gel-filtration (Sephadex G-200) techniques

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