Zoonotic and host-adapted genotypes of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in dairy cattle in Hebei and Tianjin, China

Hu, Suhui; Liu, Zhenzhen; Yan, Fengbin; Zhang, Zhenjie; Zhang, Guiling; Zhang, Longxian; Jian, Fuchun; Zhang, Sumei; Ning, Changshen; Wang, Rongjun

doi:10.1016/j.vetpar.2017.10.024

Cited by 63 publications

(40 citation statements)

References 49 publications

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“…The overall infection rate for Cryptosporidium is lower than the average prevalence of 19.5% reported previously in pre-weaned cattle in China [5], but similar to the infection rate reported in Xinjiang (15.6%) [17], and much higher than the rate in Hebei and Tianjin, China (1.0%) [7]. Prevalence of Cryptosporidium was significantly different ( X 2 = 4.924, df = 1, p = 0.026) between intensive feeding and free-ranging farms in this study, which suggests that cultivation scale may lead to differences in infection rates with Cryptosporidium .…”

Section: Resultssupporting

confidence: 73%

“…In this study, the overall infection rate in southwestern China was close to the prevalence in northwestern (9.7% [18]), northeastern (13.3% [13]) and north China (7.1% [7]), but much lower than the infection rates in central (17.6% [26]) and southeastern (60.1% [29]) China. Prior to the present study, these results were interpreted as related to differences in geographic distribution, environmental management and cultivation scale [7, 13, 18, 26, 29]. Cattle were kept in groups or in free stalls, which might promote the transmission of G. duodenalis infection among animals and lead to the high infection rates [26, 29].…”

Section: Resultssupporting

confidence: 51%

“…Its prevalence shows substantial differences, ranging from 7.1% to 60.1% in other studies in China [7, 13, 18, 26, 29]. In this study, the overall infection rate in southwestern China was close to the prevalence in northwestern (9.7% [18]), northeastern (13.3% [13]) and north China (7.1% [7]), but much lower than the infection rates in central (17.6% [26]) and southeastern (60.1% [29]) China. Prior to the present study, these results were interpreted as related to differences in geographic distribution, environmental management and cultivation scale [7, 13, 18, 26, 29].…”

Section: Resultsmentioning

confidence: 96%

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Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

et al. 2018

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Giardia duodenalis and Cryptosporidium spp. are common human and animal pathogens. They have increasingly been reported in dairy calves in recent years; however, multilocus genotyping information for G. duodenalis and Cryptosporidium infecting pre-weaned dairy calves in southwestern China is limited. In the present study, the prevalence of G. duodenalis and Cryptosporidium spp. in pre-weaned dairy calves in central Sichuan province was determined and the pathogens were analyzed molecularly. Of 278 fecal samples from pre-weaned dairy calves, 26 (9.4%) were positive for G. duodenalis and 40 (14.4%) were positive for Cryptosporidium spp. Cryptosporidium bovis (n = 28), Cryptosporidium ryanae (n = 5) and Cryptosporidium parvum (n = 7) were detected. All seven C. parvum isolates were successfully subtyped based on the gp60 gene sequence, and only IIdA15G1 was detected. Multilocus sequence typing of G. duodenalis based on beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes revealed 19 different assemblage E multilocus genotypes (two known and 17 unpublished genotypes). Based on eBURST analysis, a high degree of genetic diversity within assemblage E was observed in pre-weaned dairy calves in Sichuan province. To the best of our knowledge, this is the first study using multilocus sequence typing and eBURST analysis to characterize G. duodenalis in pre-weaned dairy calves in southwestern China.

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Section: Resultssupporting

confidence: 73%

Section: Resultssupporting

confidence: 51%

Section: Resultsmentioning

confidence: 96%

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Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

et al. 2018

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“…This infection rate, however, is higher than some studies previously conducted in Henan (6.4%, 19/298) and northeastern China (6.0%, 32/537), and lower than a large survey of E. bieneusi in cattle in Hebei (19.4%, 202/1040) (Hu et al. ; Ma et al. ).…”

Section: Discussionmentioning

confidence: 98%

The Potential Role of Synanthropic Rodents and Flies in the Transmission of Enterocytozoon bieneusi on a Dairy Cattle farm in China

Zhao

et al. 2018

J Eukaryotic Microbiology

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Enterocytozoon bieneusi causes microsporidiosis, a condition with complex epidemiology involving both direct and indirect transmission routes. To assess the potential role of synanthropic rodents and flies in the transmission of this pathogen, a total of 277 cattle fecal samples, 199 synanthropic rodents, and 50 batches of 20 flies were collected from a cattle farm. These samples were screened for the presence of E. bieneusi by PCR and sequencing of the internal transcribed spacer (ITS) region of the rRNA gene. The positive rates of cattle, synanthropic rodents, and flies were 11.9% (33/277), 4.0% (8/199) and 12.0% (6/50), respectively. Nineteen genotypes were identified, including 11 known genotypes (BEB6, I, COS-I, EbpC, D, J, CHS5, CHG1 to CHG3 and CHG14) and eight novel genotypes (named CHC9 to CHC16). The dominant genotype detected in the present study, BEB6, was found in all three categories of hosts. Moreover, human pathogenic genotypes D and EbpC were also observed in both synanthropic rodents and flies. These results demonstrate that synanthropic rodents and flies may act as biological disseminator or mechanical vector in the transmission of microsporidiosis to humans. Efforts should be made to minimize threats from these commensal animals to public health.

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“…reported subtype worldwide [32]. Subtype family IId was described from Sweden [33], Egypt [34], China [35] and Malaysia [36].…”

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confidence: 99%

Molecular characterization of Giardia intestinalis and Cryptosporidium parvum from calves with diarrhoea in Austria and evaluation of point-of-care tests

Lichtmannsperger

Hinney

Joachim

et al. 2019

Comparative Immunology, Microbiology and Infectious Diseases

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A B S T R A C TTo obtain information about the occurrence and genotype distribution of G. intestinalis and C. parvum in Austrian cattle, faecal samples from diarrhoeic calves younger than 180 days of age originating from 70 farms were examined. Of the 177 faecal samples, 27.1% were positive for Giardia cysts (immunofluorescence microscopy) and 55.4% for Cryptosporidium oocysts (phase-contrast microscopy). Positive samples were characterized by nested PCR for Giardia, 83.3% (triosephosphate isomerase; tpi) and 89.6% (β-giardin; bg) were positive, while the Cryptosporidium nested PCR returned 92.5% (60-kDa glycoprotein) positive results. Sequence analysis revealed one assemblage A-positive sample and 30 (bg) respectively 29 (tpi) assemblage E-positive samples for G. intestinalis. For C. parvum four subtypes within the IIa family (IIaA15G2R1, n = 29; IIaA19G2R2, n = 3; IIaA21G2R1, n = 2; IIaA14G1R1, n = 1) could be differentiated. Validation of two immunochromatographic point-of-care tests resulted in a sensitivity of 29.2% and 77.6%; a specificity of 98.4% and 91.1% for the detection of Giardia intestinalis and Cryptosporidium parvum, respectively. Results confirm the widespread occurrence of both protozoa in diarrhoeic calves in Austria.

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Zoonotic and host-adapted genotypes of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in dairy cattle in Hebei and Tianjin, China

Cited by 63 publications

References 49 publications

Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China

The Potential Role of Synanthropic Rodents and Flies in the Transmission of Enterocytozoon bieneusi on a Dairy Cattle farm in China

Molecular characterization of Giardia intestinalis and Cryptosporidium parvum from calves with diarrhoea in Austria and evaluation of point-of-care tests

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