Zoonotic potential of Enterocytozoon genotypes in humans and pigs in Thailand

Prasertbun, Rapeepun; Mori, Hirotake; Pintong, Ai-rada; Sanyanusin, Suparut; Popruk, Supaluk; Komalamisra, Chalit; Changbunjong, Tanasak; Buddhirongawatr, Ruangrat; Sukthana, Yaowalark; Mahittikorn, Aongart

doi:10.1016/j.vetpar.2016.12.002

Cited by 30 publications

(32 citation statements)

References 79 publications

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“…This rate is lower than the documented prevalence of E. bieneusi for: wild boars in Sichuan province, China (41.2%), domestic pigs in Jilin province, China (45.1%), wild boars in central Europe (33.3%) and pigs in the State of Rio de Janeiro, Brazil (59.3%) [14, 15, 22, 23] . However, infection rates recorded in this study were higher than those for pigs in Jilin, China (16.4%), central Thailand (28.1%) and Japan (30%)[14, 16, 22]. Differences in infection rates between these studies may be largely attributable to climate and farming mode.…”

Section: Discussioncontrasting

confidence: 72%

“…They may act as reservoirs for E. bieneusi spores and zoonotic transmission of disease. Although much research has been carried out on E. bieneusi [14–16], few studies have examined its epidemiology or Tibetan pig-associated genomes in China[17, 18], and Tibetan pigs in southwestern China have been entirely unstudied. Therefore, this study aimed to establish the incidence and molecular characteristics of E. bieneusi in Tibetan pigs, to use ITS and MLST to evaluate its genetic diversity, and to assess the potential for zoonotic transmission of microsporidiosis between Tibetan pigs and humans.…”

Section: Introductionmentioning

confidence: 99%

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First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China

Luo

Xiang

Liu

et al. 2018

Preprint

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24Enterocytozoon bieneusi is a common intestinal pathogen and a major cause of 25 diarrhea and enteric diseases in a variety of animals. While the E. bieneusi genotype 26 has become better-known, there are few reports on its prevalence in the Tibetan pig. 27 This study investigated the prevalence, genetic diversity, and zoonotic potential of E. 28 bieneusi in the Tibetan pig in southwestern China. Tibetan pig feces (266 samples) 29 were collected from three sites in the southwest of China. Feces were subjected to 30 PCR amplification of the internal transcribed spacer (ITS) region. E. bieneusi was 31 detected in 83 (31.2%) of Tibetan pigs from the three different sites, with 25.4% in 32 Kangding, 56% in Yaan and 26.7% in Qionglai. Age group demonstrated the 33 prevalence of E. bieneusi range from 24.4%( aged 0 to 1 years) to 44.4%( aged 1 to 2 34 years). Four genotypes of E. bieneusi were identified: two known genotypes EbpC 35 (n=58), Henan-IV (n=24) and two novel genotypes, SCT01 and SCT02 (one of each). 36Phylogenetic analysis showed these four genotypes clustered to group 1 with zoonotic 37 potential. Multilocus sequence typing (MLST) analysis three microsatellites (MS1, 38 MS3, MS7) and one minisatellite (MS4) revealed 47, 48, 23 and 47 positive 39 specimens were successfully sequenced, and identified ten, ten, five and five 40 genotypes at four loci, respectively. This study indicates the potential danger of E. 41 bieneusi to Tibetan pigs in southwestern China, and offers basic data for preventing 42 and controlling infections.43 44 45 Microsporidia are obligate intracellular eukaryotic pathogens, classified as fungi, 46 which are composed of approximately 1300 species in 160 genera[1]. To date, 17 47 microsporidia species are known to infect humans, and of these, E. bieneusi is the 48 most prevalent, accounting for over 90% of cases of human microsporidiosis[2]. 49 Since its first detection in an HIV/AIDS patient in 1985, a growing literature attests to 50 E. bieneusi expanding range of hosts [3-5]. In humans, infection by microsporidia 51 results in self-limiting diarrhea and malabsorption, most seriously, 52 immunocompromised and immunocompetent patients more susceptible to E. bieneusi 53 infection[6]. Normally, fecal-oral routes serve as the main infection pathways in 54 humans and animals, while human inhalation of E. bieneusi spores has also been 55 documented[7, 8]. 56 PCR-based molecular techniques may be used to analyze the E. bieneusi genome, 57 and for diagnosis. Based on the nested PCR amplification of internal transcribed 58 spacers (ITS) of small subunits of ribosomal rRNA (SSU rRNA), over 240 E. 59bieneusi genotypes have been identified globally [9][10][11]. Phylogenetic analysis reveals 60 that these genotypes clustered into nine groups. Group 1 is considered zoonotic, and is 61 composed of genotypes from humans and a few animals, while groups 2-9 have 62 particular host associations or are found in wastewater [5, 11]. To better comprehend 63 E. bieneusi genetic diversity and molecu...

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Section: Discussioncontrasting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China

Luo

Xiang

Liu

et al. 2018

Preprint

View full text Add to dashboard Cite

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“…Cameroon (Breton et al, 2007) Gabon (Breton et al, 2007) Peru (Bern et al, 2005;Cama et al, 2007;Sulaiman et al, 2003a) England (Sadler et al, 2002) Niger, Vietnan (Espern et al, 2007) Nigeria (Akinbo et al, 2012;Ayinmode et al, 2011;Maikai et al, 2012) Malawi, Netherlands (ten Hove et al, 2009) Iran (Agholi et al, 2013a;Agholi et al, 2013b) Thailand (Leelayoova et al, 2006;Prasertbun et al, 2017;Saksirisampant et al, 2009) Russia (Sokolova et al, 2011) Congo (Wumba et al, 2012) China (Wang et al, 2013a,b) Portugal (Lobo et al, 2012) Tunisia (Chabchoub et al, 2012) Poland (Kicia et al, 2014) India (Li et al, 2013) Democratic Republic of São Tomé and Príncipe (Lobo et al, 2014) Brazil (Feng et al, 2011) Spain (Galván et al,…”

Section: Humanmentioning

confidence: 99%

“…United States of America (Buckholt et al, 2002) Japan (Abe and Kimata, 2010) Thailand (Prasertbun et al, 2017) China (Li et al, 2014a;Zhao et al, 2014b) Czech Republic (Sak et al, 2008) Cattle South Africa (Abu Samra et al, 2012) China (Li et al, 2016a;Qi et al, 2017;Zhao et al, 2015c) Argentina (Del Coco et al, 2014) Brazil (Fiuza et al, 2016b) Korea (Lee 2007(Lee , 2008) Sheep…”

Section: Pigmentioning

confidence: 99%

Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

Prado

Ramos

Fiúza

et al. 2019

Rev. Bras. Parasitol. Vet.

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Enterocytozoon bieneusi is an opportunistic intestinal pathogen that infects humans and a wide variety of animals worldwide. Our aim in this study was to investigate the occurrence of E. bieneusi in a domestic cat population in Campo Grande, Mato Grosso do Sul, Brazil. Sixty fecal samples from diarrheic cats were subjected to polymerase chain reaction (PCR) and the amplicons were sequenced for identification. E. bieneusi was detected in two samples (3.3%), both identified as genotype D. This genotype has already been reported in animals and humans and is considered a zoonotic genotype. Our findings represent the first report of E. bieneusi in domestic cats in Brazil, reinforcing the importance of identifying this agent as a source of infection in animals and humans.

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“…It is ubiquitous in the environment and is responsible for over 90% of intestinal microsporidiosis in humans ( Desportes et al, 1985 ). Transmission of E. bieneusi was mainly through fecal-oral route, such as ingestion of food and/or water contaminated by spores of E. bieneusi ( Prasertbun et al, 2017 ). AIDS infection with this pathogen may cause life-threatening chronic diarrhea ( Canning and Hollister, 1990 ).…”

Section: Introductionmentioning

confidence: 99%

Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern

Zhang

Jiang

et al. 2018

Front. Microbiol.

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Enterocytozoon bieneusi is the most important causative agent of microsporidiosis and can infect almost all vertebrate and invertebrate hosts, including minks (Neovison vison). In the present study, a total of 298 feces samples (including 79 from Heilongjiang province, 31 from Hebei province, 67 from Jilin province, 90 from Liaoning province, and 31 from Shandong province, Northern China) were examined by nested PCR amplification of the internal transcribed spacer (ITS) region of the rRNA gene. The overall prevalence of E. bieneusi in minks was 10.1%, with 10.5% in Jilin province, 32.3% in Hebei province, 8.9% in Liaoning province, 0% in Shandong province, and 6.3% in Heilongjiang province. Furthermore, multiple logistic regression analysis revealed that region was only risk factors associated with E. bieneusi infection in the investigated minks. Five E. bieneusi ITS genotypes (three known genotypes, namely D, Peru11, and EbpC; two novel genotypes, namely, NCM-1 and NCM-2) were found in the current study. Importantly, genotypes D, Peru11 and EbpC, previously identified in humans, were also found in minks, which suggested that minks are the potential sources of human microsporidiosis. To the best of our knowledge, this is the first report of E. bieneusi infection in minks worldwide. The results of the present survey have implications for the controlling E. bieneusi infection in minks, other animals and humans.

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Zoonotic potential of Enterocytozoon genotypes in humans and pigs in Thailand

Cited by 30 publications

References 79 publications

First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China

First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China

Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern

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