Zoonotic Transmission of Cryptosporidium and Microsporidia in Individuals of the Kurdistan Province, West of Iran

Bahrami, Fatemeh; Haghighi, Ali; Zamini, Ghasem; Erfan, Mohammad Bagher Khadem

doi:10.1645/18-99

Cited by 6 publications

(3 citation statements)

References 26 publications

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“…( 24 ). Similar to our results, molecular investigation of collected stool samples in a study in Kurdistan Province, West of Iran, in 2020, identified the isolates as C. parvum and therefore suggested a zoonotic transmission in the area ( 25 ). In contrast, the results of other studies conducted in Isfahan, Iran ( 12 ) and in Australia ( 26 ) showed that C. hominis was more common than C. parvum ( 20 ).…”

Section: Discussionsupporting

confidence: 87%

Identification and Subtyping of Cryptosporidium parvum and Cryptosporidium hominis in Cancer Patients, Isfahan Province, Central Iran

Pestechian¹,

Manesh²,

Tavakoli³

et al. 2022

IJPA

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Background: Cryptosporidium spp. are protozoan parasites that cause diarrhea in humans and animals. Subtyping data about Cryptosporidium spp. in Isfahan, Iran is limited; therefore, we aimed to study the prevalence rate of Cryptosporidium spp. in cancer patients, associated risk factors, and subtypes of Cryptosporidium spp. Methods: Fecal samples were collected from 187 cancer patients from the Oncology Department of Seyed-al-Shohada Hospital, Isfahan University of Medical Sciences during 2014–2020 and screened for Cryptosporidium spp. using microscopical techniques. Nested PCR amplifying 18S rRNA gene was used to detect Cryptosporidium spp. in samples, followed by subtyping using nested PCR amplifying gp60 sequences. Results: Overall, the rate of infection with Cryptosporidium spp. was 4.3% (n=8). Five samples out of eight samples were identified as Cryptosporidium spp. using a nested PCR for the 18S rRNA gene, two subtypes of C. parvum named IIaA18G3R1 (n = 2) and IIaA17G2R1 (n = 2), and one subtype of C. hominis named IbA6G3 were identified by sequencing of the gp60. The IbA6G3 subtype has rarely been detected in other investigations. Conclusion: This is the first survey on the subtyping of Cryptosporidium spp. in this region. The results of the present survey show both zoonotic and anthroponotic transmission routes in the region.

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Section: Discussionsupporting

confidence: 87%

Identification and Subtyping of Cryptosporidium parvum and Cryptosporidium hominis in Cancer Patients, Isfahan Province, Central Iran

Pestechian¹,

Manesh²,

Tavakoli³

et al. 2022

IJPA

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“…isolated from different hosts [7,8]. At present, 38 subtypes (STs) have been identified (ST1-ST38) [9][10][11], 14 of which have been isolated from both humans and animals worldwide [12][13][14], while the other STs have been reportedly found only in animals [15,16]. Researchers have found that STs 1-4 appear to be the most common Blastocystis inhabitants of the human intestines, and the other 10 STs are rare in humans but are frequently detected in various animal groups including birds and hoofed animals [1,16,17].…”

Section: Introductionmentioning

confidence: 99%

“…At present, 38 subtypes (STs) have been identified (ST1-ST38) [9][10][11], 14 of which have been isolated from both humans and animals worldwide [12][13][14], while the other STs have been reportedly found only in animals [15,16]. Researchers have found that STs 1-4 appear to be the most common Blastocystis inhabitants of the human intestines, and the other 10 STs are rare in humans but are frequently detected in various animal groups including birds and hoofed animals [1,16,17]. Molecular identification studies of Blastocystis STs provide a discriminating tool for investigating the epidemiology of the parasite including transmission route, host specificity, and chemotherapeutic drug resistance [18,19].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Blastocystis subtypes in symptomatic patients from the southern region of Syria

2023

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Blastocystis sp. is an enteric protist found in humans and a wide range of animal hosts. Genetic variations were established among the 38 different subtypes detected so far, 14 of which are commonly found in human and animal hosts. The aim of the present study is to estimate the prevalence of the common Blastocystis subtypes and evaluate the possible correlation with several variables (gender, age, symptoms, domestic animals…), among patients from the southern region of Syria. Fecal samples were collected from individuals suffering from gastrointestinal complaints. Microscopic examination along with genotype analyses using seven pairs of subtype-specific primers was performed. Our results revealed the presence of Blastocystis sp. in 46 isolates out of the 60 samples microscopically studied (76.7%); single infection was detected in 24 isolates whereas co-infection with other protozoa was identified in 22 ones. Molecular detection targeting the SSU rRNA gene revealed a 100% positive presence of Blastocystis sp. in all the samples. Genotyping results detected the presence of five different subtypes (ST1-ST5) with varying proportions. However, ST1 was the dominant subtype observed (66.7%). Mixed subtype infections were found in 9 isolates (15%). Three samples remained undefined, nonetheless. Our statistical results showed no significant correlation between Blastocystis STs infection and the different studied variables. In conclusion, this study provides the first genetic characterization of Blastocystis subtypes prevalence in patients from the southern region of Syria. ST1 distribution was highly predominant. Further molecular studies are needed to estimate the prevalence of Blastocystis sp. infection in other regions in Syria and to understand the epidemiology and sources of transmission to humans.

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A Systematic Review and Meta-analysis on the Global Molecular Epidemiology of Microsporidia Infection Among Rodents: A Serious Threat to Public Health

et al. 2021

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Zoonotic Transmission of Cryptosporidium and Microsporidia in Individuals of the Kurdistan Province, West of Iran

Cited by 6 publications

References 26 publications

Identification and Subtyping of Cryptosporidium parvum and Cryptosporidium hominis in Cancer Patients, Isfahan Province, Central Iran

Identification and Subtyping of Cryptosporidium parvum and Cryptosporidium hominis in Cancer Patients, Isfahan Province, Central Iran

Molecular characterization of Blastocystis subtypes in symptomatic patients from the southern region of Syria

A Systematic Review and Meta-analysis on the Global Molecular Epidemiology of Microsporidia Infection Among Rodents: A Serious Threat to Public Health

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