Zscan5b Deficiency Impairs DNA Damage Response and Causes Chromosomal Aberrations during Mitosis

Ogawa, Seiji; Yamada, Mitsutoshi; Nakamura, Akihiro; Sugawara, Tohru; Nakamura, Akinori; Miyajima, Shoko; Harada, Yuichirou; Ooka, Reina; Okawa, Ryuichiro; Miyauchi, Jun; Tsumura, Hideki; Yoshimura, Yasunori; Miyado, Kenji; Akutsu, Hidenori; Tanaka, Mamoru; Umezawa, Akihiro; Hamatani, Toshio

doi:10.1016/j.stemcr.2019.05.002

Cited by 8 publications

(3 citation statements)

References 40 publications

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“…In our case, that means that it is possible that we could (or not) find a subtle phenotype in animals lacking the sixteen Oog genes such as less primordial follicles (because of an impairment in the constitution of the pool of the ovarian reserve), accelerated ovarian aging with early menopause as in AMH -/mice [29], impaired recruitment of ovarian follicles due to disturbed functioning of tyrosine kinase signaling pathway (linked to RAS), etc... More generally, it is also possible that some of the known dispensable genes prove to be crucial only under extreme experimental conditions. For example, embryonic stem cells from Zsacn5b KO females showed an impaired DNA damage response after irradiation, compared to controls [30]. Moreover, genetic background may influence the dispensability of genes.…”

Section: Discussionmentioning

confidence: 99%

Sixteen oogenesin genes are dispensable for fertilityWhat is the significance of the dispensability of genes expressed in germ-cell?

Castille

Thépot

Fouchécourt

et al. 2023

Preprint

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Gene knockout experiments have shown that many genes are dispensable for a given biological function. The Oogenesin/Pramel family contains almost 85 paralogs, about thirty of which are specific to female (as well as male for some of them) germ cells. In this paper, we show that the deletion of a block of around 1Mb containing sixteen paralogous genes of the Oogenesin/Pramel family specific to germ cells, including Oogenesin-2, -3 and -4, has no consequences on fertility or prolificacy in mouse both sexes. The dispensability of these genes is probably due to the compensation by the other germ-cell specific paralogs.

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Section: Discussionmentioning

confidence: 99%

Sixteen oogenesin genes are dispensable for fertilityWhat is the significance of the dispensability of genes expressed in germ-cell?

Castille

Thépot

Fouchécourt

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Mitotic DNA repair is thought to primarily involve sister chromatids. Abnormal mitotic DNA repair is closely related to tumorigenesis and progression [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis of key candidate genes and signaling pathways in ovarian cancer by bioinformatics

Dong

Tian

et al. 2021

J Ovarian Res

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Background Ovarian cancer is one of the most common gynecological tumors, and among gynecological tumors, its incidence and mortality rates are fairly high. However, the pathogenesis of ovarian cancer is not clear. The present study aimed to investigate the differentially expressed genes and signaling pathways associated with ovarian cancer by bioinformatics analysis. Methods The data from three mRNA expression profiling microarrays (GSE14407, GSE29450, and GSE54388) were obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes between ovarian cancer tissues and normal tissues were identified using R software. The overlapping genes from the three GEO datasets were identified, and profound analysis was performed. The overlapping genes were used for pathway and Gene Ontology (GO) functional enrichment analysis using the Metascape online tool. Protein–protein interactions were analyzed with the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING). Subnetwork models were selected using the plugin molecular complex detection (MCODE) application in Cytoscape. Kaplan–Meier curves were used to analyze the univariate survival outcomes of the hub genes. The Human Protein Atlas (HPA) database and Gene Expression Profiling Interactive Analysis (GEPIA) were used to validate hub genes. Results In total, 708 overlapping genes were identified through analyses of the three microarray datasets (GSE14407, GSE29450, and GSE54388). These genes mainly participated in mitotic sister chromatid segregation, regulation of chromosome segregation and regulation of the cell cycle process. High CCNA2 expression was associated with poor overall survival (OS) and tumor stage. The expression of CDK1, CDC20, CCNB1, BUB1B, CCNA2, KIF11, CDCA8, KIF2C, NDC80 and TOP2A was increased in ovarian cancer tissues compared with normal tissues according to the Oncomine database. Higher expression levels of these seven candidate genes in ovarian cancer tissues compared with normal tissues were observed by GEPIA. The protein expression levels of CCNA2, CCNB1, CDC20, CDCA8, CDK1, KIF11 and TOP2A were high in ovarian cancer tissues, which was further confirmed via the HPA database. Conclusion Taken together, our study provided evidence concerning the altered expression of genes in ovarian cancer tissues compared with normal tissues. In vivo and in vitro experiments are required to verify the results of the present study.

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“…More generally, it is also possible that some of the dispensable genes cited in this review prove to be crucial under extreme experimental conditions. For example, embryonic stem cells from Zscan5b KO females showed an impaired DNA damage response after irradiation compared to controls [57].…”

Section: Introductionmentioning

confidence: 99%

The paralogs’ enigma of germ-cell specific genes dispensable for fertility: the case of 19 oogenesin genes

Castille

Thépot

Fouchécourt

et al. 2023

Biology of Reproduction

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Gene knockout experiments have shown that many genes are dispensable for a given biological function. In this review, we make an assessment of male and female germ cell-specific genes dispensable for the function of reproduction in mice, the inactivation of which does not affect fertility. In particular, we describe the deletion of a 1 Mb block containing nineteen paralogous genes of the oogenesin/Pramel family specifically expressed in female and/or germ cells, that has no consequences in both sexes. We discuss this notion of dispensability and the experiments that need to be carried out to definitively conclude that a gene is dispensable for a function.

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Zscan5b Deficiency Impairs DNA Damage Response and Causes Chromosomal Aberrations during Mitosis

Cited by 8 publications

References 40 publications

Sixteen oogenesin genes are dispensable for fertilityWhat is the significance of the dispensability of genes expressed in germ-cell?

Sixteen oogenesin genes are dispensable for fertilityWhat is the significance of the dispensability of genes expressed in germ-cell?

Integrative analysis of key candidate genes and signaling pathways in ovarian cancer by bioinformatics

The paralogs’ enigma of germ-cell specific genes dispensable for fertility: the case of 19 oogenesin genes

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