Zuogui Pill Attenuates Neuroinflammation and Improves Cognitive Function in Cerebral Ischemia Reperfusion-Injured Rats

Abstract

<b><i>Introduction:</i></b> Cerebral ischemia and reperfusion (CI/R) injury is a devasting cerebrovascular disease, accompanied with ischemia stroke, cerebral infarction. Zuogui Pill (ZGP), as a Chinese traditional medicine, is proved to be effective in many diseases and cancers. Our study aimed to detect the roles of ZGP in CI/R injury. <b><i>Methods:</i></b> Neural stem cells were isolated from rats and induced by oxygen and glucose deprivation and recovery. CCK-8 and flow cytometry were applied to assess the function of ZGP on cell viability and apoptosis. Rat CI/R injury models were established by the middle cerebral artery occlusion and reperfusion. The function of ZGP on CI/R injury was identified via evaluating modified neurological severity score, infarct area, and cognitive impairment. <b><i>Results:</i></b> Compared to the control, the cell viability was obviously decreased in the oxygen and glucose deprivation and recovery (OGD/R) group, while the adverse influence on cells was reversed by cultured plus 10% ZGP serum. Consistently, ZGP attenuated the influence of OGD/R on cell apoptosis. More importantly, ZGP could alleviate CI/R injury of rats by reducing neurological damage and infarct area and promoting cognitive function. <b><i>Conclusion:</i></b> This study provided protective roles of ZGP on cell viability and apoptosis induced by OGD/R. In addition, ZGP played protective roles on neuroinflammation and cognitive function in rats.

Exploring the mechanisms under Zuogui Pill’s treatment of ischemic stroke through network pharmacology and in vitro experimental verification

“…Our in vitro experiments in this study demonstrated that ZGP protected ischemic neurons by increasing the cell viability of differentiated SH-SY5Y cells following OGD/R. Several other investigations have revealed that the mechanism behind ZGP’s protective impact on ischemic stroke is related to inhibit oxidative stress and inflammatory reaction and regulate PI3K/Akt pathway ( Liu et al, 2017 ; Liu et al, 2021 ; Liu et al, 2022 ). However, the effect and mechanism of ZGP on neurite growth and regeneration have not been experimentally verified.…”

Deciphering Post-Stroke Sleep Disorders: Unveiling Neurological Mechanisms in the Realm of Brain Science

“…Cerebral ischemia alters cerebrovascular hemodynamics, thus affecting oxygen and nutrient supply to the brain, with profound implications for nervous system function [62]. This impairment transcends cognitive and motor functions, extending into the modulation and stage of sleep [63,64]. Recent studies highlighted the intricate link amongst cerebral ischemia, cerebrovascular hemodynamics and sleep [65,66].…”