Zur Bildung pektolytischer und cellulolytischer Enzyme durch Cercosporella herpotrichoides Fron.

Hänssler, G.

doi:10.1111/j.1439-0434.1973.tb04127.x

Cited by 5 publications

(1 citation statement)

References 16 publications

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“…The fungi, maintained in tubes with soil were taken in culture by transferring them to SNA medium. To determine colony morphology, mycehal plugs from actively growing colonies were put on PDA and incubated for 2 weeks at 20 °C under UV hght (350 nm), Freeze-dried mycelium harvested from hquid culture medium (HANSSLER 1973) was ground to a fine powder and incubated with double-concentrated CTAB buffer (SAGHAI-MAROOF etal 1984) for 1,5 h at 65 °C in a shaking water bath. After twice extracting with chloroform, the DNA was precipitated with 2-propanol, recovered with a Pasteur pipette, washed in 70 % ethanol, air-dried and dissolved in TE buffer.…”

Section: Methodsmentioning

confidence: 99%

Differentiation and Diagnosis of Pseudocercosporella herpotrichoides (Fron) Deighton with Genomic DNA Probes

Frei¹,

Wenzel²

1993

Journal of Phytopathology

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Repetitive genomic clones were used to differentiate between varieties within the species Pseudocercosporella herpotrichoides. From 21 clones tested 13 revealed restriction fragment length polymorphisms among isolates. Cluster analysis was performed based on these data. Differentiation of isolates according to growth speed corresponds better to genetic information than differentiation on the basis of spore morphology. Pathogen‐specific probes can be used in early diagnosis of the pathogen in infected plant material. Furthermore polymorphic pathogen‐specific probes allow varieties to be differentiated directly in infected plant material without isolation and culture of the fungus.

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Section: Methodsmentioning

confidence: 99%