Zur biosynthese der anthtranilsäure in Saccharomyces cerevisiae

Pathways of biosynthesis of aromatic amino acids and vitamins and their control in microorganisms.

“…Because of the complexity of the reaction catalyzed by anthranilate synthetase, intermediates between chorismate and anthranilate have been proposed (102,108,136,151). The enzymic evidence suggests that a protein com-GLUTAMIEW HO().COOH (.WMH) Mg l 9 CHC-COOH A CHORISMIC ACID plex metabolizes chorismate through anthranilate to N-(5'-phosphoribosyl)-anthranilate.…”

“…Although the structure of the hydroxamate is not yet known, the evidence obtained suggests that the overall conversion of chorismate to anthranilate may be separable into two steps, the first utilizing glutamine and the second being dependent on Mg2+. Several possible intermediates have been tested, but they do not serve as precursors of anthranilate (108,151). Any mechanistic scheme for the amination of chorismate must take into account the finding of Srinivasan (151) that the amide nitrogen of glutamine is transferred to the carbon 2 of chorismate ( Fig.…”

Anthranilate Synthetase

“…The complexity in the conversion of chorismate into anthranilate has invited numerous speculations about the course of the reaction (63)(64)(65)(66). However chemical intermediates have not been identified.…”