Zur elektronenmikroskopischen Untersuchung der juxtaglomerul�ren Spezialeinrichtungen der Niere

Bucher, Otto; Reale, E.

doi:10.1007/bf00338701

Cited by 27 publications

(5 citation statements)

References 13 publications

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“…Others were more elliptoid or amorphous and are similar to those observed in the juxtaglomerular apparatus of the kidney (23)(24)(25)(26). It is possible that the granules lost their shape in the hypertonic sucrose solution, but hypertonic sucrose was used initially to separate zymogen particles from the pancreas (2).…”

Section: Microscopysupporting

confidence: 56%

Isolation from a Salivary Gland of Granules Containing Renin and Kallikrein

Chiang

Erdös

Miwa

et al. 1968

Circulation Research

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Granules of the submaxillary gland of the white mouse contain both kallikrein and renin. The granules were separated and concentrated in sequential centrifugation procedures. They were more stable at room temperature and in hypertonic sucrose solutions than in the cold or in isotonic solution. The amylase, acid phosphatase, renin, kallikrein, and benzoyl-L-arginine ethyl ester esterase contents of the granules were determined. Kallikrein and renin showed a similar distribution pattern after fractionation. Granular renin released the equivalent of 9. Electron micrographs of the isolated granules showed various forms; some were spherical and symmetrical, and others were amorphous. ADDITIONAL KEY WORDSsubmaxillary gland bradykinin angiotensin subcellular fractions electron microscopy differential centrifugation insoluble enzyme polymers kallidin• Granules originating from the rat submaxillary gland contain large amounts of kallikrein ( 1 ) . The appearance of these particles in electron micrographs is similar to that of pancreatic zymogen granules (2). Although the presence of renin in granules has been gaining acceptance, a definite relationship between granules and renin activity has not yet been established (3,4 ) . In addition to kallikrein, renin was found in the submaxillary glands of the white mouse (5-8). Renin activity is concentrated in the striated ducts which have granulated cells. These findings led us to study the subcellular distribution of renin and its relationship to kallikrein in the submaxillary gland of the white mouse. In the present communication we deal withFrom the Departments of Pharmacology and Pathology, University of Oklahoma, School of Medicine, Oklahoma City, Oklahoma 73104.This work was supported in part by U. S. Public Health Service Grant HE 08764 from the National Institutes of Health.When this work was done Dr. Miwa was a fellow of the Oklahoma Heart Association. His permanent address is Department of Medicine, Tohoku University, Sendai, Japan.Accepted for publication August 5, 1968.the isolation of an active, granular layer that contains both kallikrein and renin.Methods White male Swiss Webster mice (A. Sutter Co.) were killed by a sudden blow on the head without using anesthesia. The mice were over 90 days old and weighed about 50 g. Fresh, minced submaxillary glands were homogenized in batches of six pairs in a Dounce hand homogenizer. In the first series of experiments, the tissues were placed in a medium of 0.25M sucrose solution buffered with 0.005M Tris, pH 7.4, in an ice bath. A 10% (w/v) homogenate was prepared and filtered through four layers of gauze. The filtered homogenate was then fractionated using isotonic sucrose solutions and a refrigerated centrifuge as previously described (1).In the second series of experiments, all procedures were done at room temperature in a 0.88M sucrose solution buffered with 0.005M Tris, pH 7.4. The unbroken cells and tissues were removed by centrifugation at 120 g for 5 minutes using a Sorvall RC-2 centrifuge. The step was rep...

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Section: Microscopysupporting

confidence: 56%

Isolation from a Salivary Gland of Granules Containing Renin and Kallikrein

Chiang

Erdös

Miwa

et al. 1968

Circulation Research

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“…The cytological origin of renin-containing cells has been discussed by many investigators [26,27], In the present study, renin-containing granules were observed in the mesangial cells and smooth muscle cells by immunoelectron-microscopical techniques. These results suggest the possibility that these cells of mesenchymal origin are able to differentiate and develop the capacity for renin production.…”

Section: Discussionmentioning

confidence: 62%

An Immunoelectron-Microscopical Observation of Mouse Juxtaglomerular Cells in the Case of Experimental Hydronrophrosis

Kon

Hashimoto

Murakami

et al. 1992

Cells Tissues Organs

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Changes in juxtaglomerular (JG; renin-containing) cells in experimental hydronephrosis 1 month after ureteral ligation were investigated with immunoelectron-microscopical techniques. Two types of granules, electron dense (D) and lucent (L), were observed. D type granules were labeled more intensely with gold particles than those of L type. Granules intermediate between D and L types and exocytosis of D types were observed. In the cells containing D types exclusively, gold particles were restricted to the granules, whereas in the cells containing both D and L type granules, the particles were scattered throughout the cytoplasmic cytosol. The authors discuss the mechanisms of renin release in JG cells.

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“…It has been suggested that mesangial cell contraction may act, at least in part, as an effector mechanism of the tubuloglomerular feedback (TGF), i.e., the regulation ofglomerular filtration by distal tubule fluid via macula densa (27,28), and that a change in the luminal [Cl-] at macula densa signals the TGF (27,29). Between macula densa and the glomerulus, there lie Goormaghtigh cells or lacis cells, which are interstitial cells morphologically indistinguishable from mesangial cells and thus are called extraglomerular mesangial cells (30). Goormaghtigh cells, mesangial cells, and juxtaglomerular cells are coupled with each other by gap junctions, thus forming a syncytium (31).…”

Section: Effects Ofextracellular Chloride Ion Concentrationmentioning

confidence: 99%

“…Goormaghtigh cells, mesangial cells, and juxtaglomerular cells are coupled with each other by gap junctions, thus forming a syncytium (31). The area surrounding Goormaghtigh cells are neither well-perfused with capillaries nor drained with lymph vessels (27,30). Therefore, it is highly possible that [CL-]0 of these cells may be directly altered by a change in (27,29).…”

Section: Effects Ofextracellular Chloride Ion Concentrationmentioning

confidence: 99%

Ambient C1- ions modify rat mesangial cell contraction by modulating cell inositol trisphosphate and Ca2+ via enhanced prostaglandin E2.

Okuda

Kojima²,

Ogata³

et al. 1989

J. Clin. Invest.

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Our recent observation showed that angiotensin II (All) and arginine vasopressin (AVP) stimulate Ca2 -activated Cl-conductance in mesangial cells. These data raise the possibility that mesangial cell function may be modulated by extracellular chloride concentration [Cl]0 decreased and the effects of ambient Cl-deprivation could be restored by addition of indomethacin to the Cl--free medium. Moreover, PGE2 decreased mesangial cell contractility, Ca2+ transients, and IP3 production in response to All and AVP. These data suggest that the decrease in [CI-]0 attenuates mesangial cell contraction by suppressing IP3 production and thus Ca2+ transients in response to All and AVP through enhanced PGE2 production.

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Zur elektronenmikroskopischen Untersuchung der juxtaglomerul�ren Spezialeinrichtungen der Niere

Cited by 27 publications

References 13 publications

Isolation from a Salivary Gland of Granules Containing Renin and Kallikrein

Isolation from a Salivary Gland of Granules Containing Renin and Kallikrein

An Immunoelectron-Microscopical Observation of Mouse Juxtaglomerular Cells in the Case of Experimental Hydronrophrosis

Ambient C1- ions modify rat mesangial cell contraction by modulating cell inositol trisphosphate and Ca2+ via enhanced prostaglandin E2.

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