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Two wethers (28 kg and 33 kg) were supplied with ileocaecal re-entrance cannulae and received a straw pellet ration rich in crude fibre (70.5% straw, 12% chopped sugar beet, 10% cereals, 2% urea, 3% NH4HCO3 and 2.5% of a mineral mixture). In a preliminary period 50% of the digesta flow was collected on 6 successive days for 18 h each. An amount of digesta sufficient for 24 h was apportioned for hourly application and stored at a temperature of -20 degrees C for the main trial. In the main trial the two animals received intracaecally the collected digesta with a supplement of ca. 6 g hay damaged by heat/kg LW(0.75) in hourly portions over 24 h (hay made up ca. 15 and 20% resp. of the DM amount). In addition, each digesta sample was supplemented with 14C and 15N labelled urea (19.7.10(6) Bq 14C urea and 364 mg 15N excess from 15N urea). About 9% of the applied 15N amount was microbially utilized; the utilization quota was thus lower than after the application of partly hydrolyzed straw meal (16% in a previous trial). The 14C activity from 14C urea was quickly eliminated in the form of CO2 in the respiratory gases (at the 18th hour after the end of the infusion 70% excreted as CO2). The half-lives for the urea resulting from the semi-logarithmic decrease of the atom-% 15N excess in the blood plasma were 7.9 and 7.7 resp. 23% and 34% resp. of the applied 15N excess were excreted in urine. The excretion of radioactive carbon in urine, however, was at 2.8% and 4.3% resp. of the applied amount very low 120 h after the beginning of the trial (96 h after the end of the infusion). On the whole one can conclude from this trial that hay damaged by heat has only a low stimulating effect on microbial activity in the large intestine.
Two wethers (28 kg and 33 kg) were supplied with ileocaecal re-entrance cannulae and received a straw pellet ration rich in crude fibre (70.5% straw, 12% chopped sugar beet, 10% cereals, 2% urea, 3% NH4HCO3 and 2.5% of a mineral mixture). In a preliminary period 50% of the digesta flow was collected on 6 successive days for 18 h each. An amount of digesta sufficient for 24 h was apportioned for hourly application and stored at a temperature of -20 degrees C for the main trial. In the main trial the two animals received intracaecally the collected digesta with a supplement of ca. 6 g hay damaged by heat/kg LW(0.75) in hourly portions over 24 h (hay made up ca. 15 and 20% resp. of the DM amount). In addition, each digesta sample was supplemented with 14C and 15N labelled urea (19.7.10(6) Bq 14C urea and 364 mg 15N excess from 15N urea). About 9% of the applied 15N amount was microbially utilized; the utilization quota was thus lower than after the application of partly hydrolyzed straw meal (16% in a previous trial). The 14C activity from 14C urea was quickly eliminated in the form of CO2 in the respiratory gases (at the 18th hour after the end of the infusion 70% excreted as CO2). The half-lives for the urea resulting from the semi-logarithmic decrease of the atom-% 15N excess in the blood plasma were 7.9 and 7.7 resp. 23% and 34% resp. of the applied 15N excess were excreted in urine. The excretion of radioactive carbon in urine, however, was at 2.8% and 4.3% resp. of the applied amount very low 120 h after the beginning of the trial (96 h after the end of the infusion). On the whole one can conclude from this trial that hay damaged by heat has only a low stimulating effect on microbial activity in the large intestine.
Green rye (fertilized with 15N) with 37.4% crude fibre, 6.2% crude protein in DM; and 0.31 Atom-%15N-excess (15N') was separated into the fractions stem, leaves and ear. In the same order the fractions had 39.3, 36.4, 27.8% crude fibre; 3.7, 8.1, 11.4% crude protein in the DM and 0.30, 0.27, 0.33 atom-%15N'. Differences in the 15N-label between different nitrogen fractions for one part of the plant (total-N, TCA-precipitable N, pepsin insoluble N) were also measured. The reasons for these results are the once head-fertilization with N and 15N and the following 15N-turnover in the plant. The disappearance rate of DM, N and 15N of different parts of the rye plant was measured using the nylon bag technique in the rumen of sheep. The disappearance rate after a 24-hours incubation period was in the order whole plant, stem, leaves and ear as follows: DM = 44.4; 40.7; 50.3 and 71.6% N = 39.0; -8.5 (N-influx); 55.0 and 80.1% 15N' = 88.4; 84.0 88.3 and 92.4% The label of the N-fraction in the residues of bags was differently reduced in the fractions of plant as a result of influx of foreign N. The largest effect was found for the residues of stem. The reason for this result was interpreted with strong processes of adsorption in the fibrous material for N-molecules and N-compounds. A negatively linear correlation was found between the measured N-disappearance after incubation and the relative differences between the values of the 15N- and N-disappearances. These relative differences achieved negligible values when the N-disappearance was about 80%. The labeling of feedstuffs with 15N is a useful method for the estimation of true N-disappearance in experiments with the nylon bag technique.
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