Zur Kontamination von Gewebekulturen mit Mykoplasmen

Witzleb, W; Blumöhr, T; Dziambor, H; Schweizer, H

doi:10.1007/bf01250179

Cited by 7 publications

(1 citation statement)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the first report of cell cultures contaminated by pleuropneumonia-like organisms [a term previously used to describe mycoplasmas (152)] there has been a multitude of such reports. Summaries of published results from various sources noted that up to 60%o of the several hundred cell lines tested were contaminated (74,105,202). In one study of 60 cell lines, 86.7% were contaminated (126).…”

Section: Contaminationmentioning

confidence: 99%

Mycoplasmas and cell cultures.

Stanbridge¹

1971

Bacteriological Reviews

118

View full text Add to dashboard Cite

Such terms are now in disfavor and the correct generic name, Mycoplasma, should be used. Recently the mycoplasmas have been classified into two separate families, the sterol-requiring Mycoplasmataceae and the sterol-nonrequiring Acholeplasmataceae (44). To the present time, over 30 named species of mycoplasmas have been included within the two families. In addition there are several unidentifiable isolates (L. Hayflick, personal communication). The above classification refers to classic (large-colony) mycoplasmas. It does not include T-strain mycoplasmas (so called, because they form tiny colonies approximately one-fourth the size of classic mycoplasma colonies) on agar. T-strain mycoplasmas were first isolated by Shepard from human urino-genital tracts (170). They have now been isolated from various animals. The T-strain group, as a whole, is unclassified at the present time. A unique requirement for urea (171), in addition to sterol requirement, for growth of these mycoplasmas has prompted the suggestion that they should be included as a separate genus in the order Mycoplasmatales (156a). MORPHOLOGY OF MYCOPLASMAS The mycoplasma organism is distinctive for its small size of 130 to 300 nm and for the lack of a cell wall, the limiting boundary being a plasma 206

show abstract

Section: Contaminationmentioning

confidence: 99%