Zwitterionic Core-Sheath Nanofibers in Antibacterial Photodynamic Therapy

Abstract

Skin wounds and their related bacterial infections are one of the issues that would seriously threaten public health. Wound dressings affording antibiofouling capability and incorporating antimicrobial agents could prevent infection and favor wound healing, which have significant clinical demands and value. Antibacterial photodynamic therapy (aPDT) induces toxic singlet oxygen to kill microbes through a photodynamic pathway that avoid the development of drug-resistant microbes. In this study, coaxial electrospinning technology was employed to fabricate an antibacterial wound dressing containing thermoplastic polyurethane (TPU) as the core and zwitterionic and cross-linkable polysulfobetaine copolymer as the sheath. The prepared nanofibrous membranes exhibited good water uptake and retention capability, excellent in vitro biocompatibility and antifouling performance. The elastic properties of TPU improved the stretching and mechanical performance of the membranes that mimic the extensibility of the human skin. Moreover, methylene blue-loaded nanofibrous membranes have shown good antibacterial photodynamic inactivation against Gram-positiveStaphylococcus aureus (99.875%, 2.90 log units inactivation) and Gram-negative Escherichia coli (99.705%, 2.53 log units inactivation) upon mild light irradiation (500 W m −2 ), while as expected, E. coli cells have shown lower susceptibility to aPDT. Overall, the zwitterionic core-sheath nanofibrous membranes with aPDT function are potentially used as a promising antibacterial wound dressing.

Antifouling Zwitterionic Nanofibrous Wound Dressing for Long-Lasting Antibacterial Photodynamic Therapy

“…The highest LE of PM-MB was observed, suggesting that the electrostatic attraction between the positively charged MB and zwitterionic PMPC moiety promoted the increase of the MB loading. This result was consistent with the literature and our previously reported study …”

“…This result was consistent with the literature 42 and our previously reported study. 43 3.3.1. In Vitro Release Behaviors and Detection of Singlet Oxygen Generation.…”

Preparation of a TPU-Based Flexible Stab-Resistant Composite Membrane Filled with SiO2 and SBR Nanoparticles

“…Thermoplastic polyurethane (TPU) is widely used in several industrial fields such as coating, adhesive, engineering material, and film. − The segmented structure of TPU, which consists of a soft segment of polymer glycol and a hard segment of the diisocyanate, gives the TPU flexibility, mechanical properties and excellent processability . Many scholars also considered the potential application of TPU in stab-resistant material .…”

Photothermally Controlled Drug Release of Poly(d,l-lactide) Nanofibers Loaded with Indocyanine Green and Curcumin for Efficient Antimicrobial Photodynamic Therapy

“…An in vivo study of polyurethane nanofibers with tetraphenylporphyrin reduced wound size and pain in chronic leg ulcers after visible light irradiation [ 22 ]. Furthermore, polymer nanofibers loaded with methylene blue were used to study antimicrobial photoactivity against bacteria [ 23 , 24 ] and the healing of infected wounds in a rat model [ 25 ].…”