Zwitterionic Cysteine Drug Coating Influence in Functionalization of Implantable Ti50Zr Alloy for Antibacterial, Biocompatibility and Stability Properties

Abstract

The present paper aims atincreasing the bioperformance of implantable Ti50Zr alloy using zwitterionic cysteine drug coating. Aspects such as stability, biocompatibility, and antibacterial effects were investigated with the help of various methods such as infrared spectroscopy (FT-IR), scanning electronic microscopy (SEM), electrochemical methods, contact angle determinations and cell response. The experimental data of zwitterionic cysteine coating indicate the existence of a hydration layer due to hydrophilic groups evidenced in FT-IR which is responsible for the decrease of contact angle and antibacterial capabilities. The electrochemical stability was evaluatedbased on Tafel plots and electrochemical impedance spectroscopy (EIS). The cell response to cysteine was determined with gingival fibroblasts measuring lactate dehydrogenase (LDH) activity, concentrations of nitric oxide (NO) and intracellular level of reactive oxygen species (ROS). All experimental results supported the increase of stability and better cells response of implantable Ti50Zr alloy coated with zwitterionic cysteine drug. The antibacterial index was measured against Staphylococcus aureus and Escherichia coli. It was demonstrated that the coating enhanced the production of intracellular ROS in time, which subsequently caused a significant increase in antibacterial index.

The Trends of TiZr Alloy Research as a Viable Alternative for Ti and Ti16 Zr Roxolid Dental Implants

“…where I% is the growth inhibition index, C 0 is the blank-corrected optical density of the positive control at time 0, C 24 is the blank-corrected optical density of the positive control after 24 h, T 0 is the blank-corrected optical density of infected media in the presence of test samples, and T 24 is the blank-corrected optical density of infected media in the presence of test samples at 24 h [41].…”

“…Based on the existing expertise, we chose to present the unmodified and modified surface properties of Ti50Zr [36][37][38] with better biocompatible antibacterial coatings. Drug loading and release from such coatings [39][40][41][42] are discussed as a part of the coated Ti50Zr performance, and future trends in dental materials applications are presented.…”

Role of Amino Acid Cysteine in the Suppression of Xanthomonas citri pv. glycines Bacterial Pustule Disease of Soybean

“…citri and Pseudomonas syringae (Goto and Yamanaka, 1981). Several clinical bacterial pathogens such as Escherichia coli, Pseudomonas fluorescens, and Salmonella enterica were also inhibited by cysteine (Harris, 1981;Tuite et al, 2005;Demetrescu et al, 2018).…”

Preparation and application of a xylan-based antibacterial papermaking additive to protect against Escherichia coli bacteria

“…Antimicrobial products based on various raw materials after modification have been extensively studied in the world (Shaer et al 2012;Poverenov et al 2013;Demetrescu et al 2018;Diaz-García et al 2019). Especially in recent years, antimicrobial products based on biological macromolecules from biomass including lignin, pectin, and different kinds of polysaccharides (e.g., cellulose, starch, and hemicellulose) have gained increasing interest due to many superior properties of these kinds of macromolecules such as biodegradability, good biocompatibility, good bioactivity, a wide range of resources from which to make the product, and easy modification of the end product (Guan et al 2008;Dutta et al 2009;Kang et al 2013).…”