Zwitterionic Ion Chromatography of Dansyl Amino Acids with 4-Vinylbenzyl Dimethyl Ammonio Pentanesulfonate as Stationary Phase

Ali

2020

ijddt

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For chromatographical separation and estimation of salbutamol sulfate, zwitterionic stationary phases with large capacity were obtained by zwitter-molecules attached to a polystyrene-divinylbenzene (PS/DVB) particle. Salbutamol sulfate retention activity was studied with eluent at various pH mobile phase, mobile phase concentrations, and acetonitrile (ACN) percentage. The methods of separation are based on separating the salbutamol sulfate into hydrophobic and cations interactions. Linearity of 0.01 to 0.9 μg.mL-1 for two columns was developed with direct calibration curves, %RSD percent (0.48 ± 0.12 and 0.49 ± 0.22), LoD (0.058 and 0.04 μg.mL-1), and LoQ (0.203 and 0.14 μg.mL-1) were created, respectively.

Section: Methodsmentioning

confidence: 99%

A Study of Retention Behavior and Method Development of Salbutamol Sulfate in Hydrophilic Interaction Chromatography

Ali

2020

ijddt

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“…ZIC1 and ZIC5 were developed on the polystyrene-divinylbenzene (PS/DVB), by means of a grafted sulfobetaine monomer (100 × 4 mm ID) and PEEK column, for the 2-deoxycytidine separation. 5,[9][10][11] The systematic cycle of grafting reaction has been established by Raskop et al 20 Chemicals 2-deoxycytidine (HPLC) purchased by Sigma-Aldrich 99%. Acetic acid was purchased from BDH.…”

Section: Instrumentation and Chromatographic Conditionmentioning

confidence: 99%

“…4 HILIC's approach to several previously intimidating separation problems is currently strongly attracted. The analysis of pharmaceuticals, [5][6][7][8] dansyl amino acids, 9 inorganic anions, 10 carboxylic acid, 11 sugar, 12 and saccharides, 13 by the successful implementation of HILIC technology has been achieved. dCD (Figure 1) is a component of deoxyribonucleic acid, and deoxyribonucleoside.…”

Section: Introductionmentioning

confidence: 99%

Investigate Retention Behavior of 2-deoxycytidine in Hydrophilic Interaction Liquid Chromatography

Hamed

2020

ijddt

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A hydrophilic interaction chromatography has been investigated to separate 2-deoxycytidine chosen for nucleoside. A small molecule with specific features for human serum samples was 2-deoxycytidine tested. 2-deoxycytidine has been applied to self-made stationary hydrophilic phases (ZIC1 and ZIC5). The deoxycytidine (dCD) retention was investigated with varying concentrations of sodium acetate buffer, acetonitrile%, and pH. The results confirmed the hydrophilicity of 2-deoxycytidine. The exchanger retention mechanism was studied taking into account 2-deoxycytidine used for describing the interaction of hydrophilic and cation. For both ZIC1 and ZIC5 exchangers, we described and discussed the influence of chromatographic conditions (concentration of sodium acetate buffer, a percent of CAN, and pH). The two methods developed are a useful alternative to current 2-deoxycytidine separation methods.

“…Hydrophilic and polar compounds, as compared to hydrophobic neutral compounds, are retained preferentially in HILIC the contrary of RP-HPLC. Thus recently, HILIC technology has thus recently begun to increase dramatically in the estimation of drugs, nucleosides, carboxylic acids, inorganic ions, and amino acids by Rasheed and its co-workers [24][25][26][27][28][29][30][31][32][33][34]. Experimental…”

Section: Introductionmentioning

confidence: 99%

Development of a validated hydrophilic interaction chromatography method for the determination of cefotaxime in pharmaceutical preparations

Kzar¹,

Hassan

2021

Egypt. J. Chem.

Cefotaxime is considered as one of the semi-synthetic, 3rd generation, cephalosporin antibiotics that have been utilized for treating a lot of infections resulting from different organisms. The technique of hydrophilic chromatography interaction (HILIC) has increased the accuracy and sensitivity concerning other methods, particularly spectrophotometer. The objective of this article was to introduce a simple method for the estimation of cefotaxime in pure and pharmaceutical injection forms and study the separation mechanism of cefotaxime. HILIC mode achieved excellent separation under chromatographic conditions on a HALO ® HILIC 2.7 column (100 mm x 2.1 mm I.D.) at 35 ° C with the following conditions: 10:90% acetate buffer (pH 5.5-40 mM): acetonitrile as eluent and wavelength of detection 254 nm. The proposed HILIC method exhibited high precision (RSD% < 0.5%), concentration ranges of 100-5500 ppb, and the lowest detection limit was 6.8167 ppb with a coefficient of determination of 0.9998 for cefotaxime. The findings of the method were used statistical tests compared to the British Pharmacopoeia protocol, which did not show any difference in accuracy among the methods