Zwitterionic peptide anchored to conducting polymer PEDOT for the development of antifouling and ultrasensitive electrochemical DNA sensor

Wang, Guixiang; Han, Rui; Su, Xiaoli; Li, Yinan; Xu, Guogang; Liu, Xiang

doi:10.1016/j.bios.2016.10.088

Cited by 123 publications

(72 citation statements)

References 28 publications

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“…Zwitterionic peptides terminated with carboxylic groups, anchored to a conducting polymer of citrate-doped PEDOT via nickel cation coordination, with remarkable antifouling ability and good conductivity, were proposed for the construction of an electrochemical DNA sensor for the determination of the BRCA1 breast cancer biomarker [41]. A layer of the conducting polymer PEDOT was electrodeposited on a GCE in a solution containing 3,4-ethylenedioxythiophene (EDOT) and sodium citrate.…”

Section: Antibiofouling Peptides and Peptoid-based Materialsmentioning

confidence: 99%

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Antifouling (Bio)materials for Electrochemical (Bio)sensing

Campuzano

Pedrero

Yáñez‐Sedeño

et al. 2019

IJMS

107

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(Bio)fouling processes arising from nonspecific adsorption of biological materials (mainly proteins but also cells and oligonucleotides), reaction products of neurotransmitters oxidation, and precipitation/polymerization of phenolic compounds, have detrimental effects on reliable electrochemical (bio)sensing of relevant analytes and markers either directly or after prolonged incubation in rich-proteins samples or at extreme pH values. Therefore, the design of antifouling (bio)sensing interfaces capable to minimize these undesired processes is a substantial outstanding challenge in electrochemical biosensing. For this purpose, efficient antifouling strategies involving the use of carbon materials, metallic nanoparticles, catalytic redox couples, nanoporous electrodes, electrochemical activation, and (bio)materials have been proposed so far. In this article, biomaterial-based strategies involving polymers, hydrogels, peptides, and thiolated self-assembled monolayers are reviewed and critically discussed. The reported strategies have been shown to be successful to overcome (bio)fouling in a diverse range of relevant practical applications. We highlight recent examples for the reliable sensing of particularly fouling analytes and direct/continuous operation in complex biofluids or harsh environments. Opportunities, unmet challenges, and future prospects in this field are also pointed out.

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Section: Antibiofouling Peptides and Peptoid-based Materialsmentioning

confidence: 99%

“…Electrochemical DNA sensor for BRCA1 determination involving the use of a zwitterionic peptide anchored to the conducting polymer PEDOT. Reproduced from [41] with permission.…”

Section: Figurementioning

confidence: 99%

Antifouling (Bio)materials for Electrochemical (Bio)sensing

Campuzano

Pedrero

Yáñez‐Sedeño

et al. 2019

IJMS

107

View full text Add to dashboard Cite

show abstract

“…Although these are considered as the “gold standard” materials, they show various defects such as their oxidative damage, poor water-solubility, and low protein resistance at high temperature. Peptides have come up as possible alternative antifouling materials in electrochemical affinity biosensors [ 30 , 31 , 32 ], providing additional advantages of biocompatibility, tunable and simple structure and synthesis [ 24 ].…”

Section: Continuous Real-time Electrochemical Biosensors: Towardsmentioning

confidence: 99%

Beyond Sensitive and Selective Electrochemical Biosensors: Towards Continuous, Real-Time, Antibiofouling and Calibration-Free Devices

Campuzano

Pedrero

Gamella

et al. 2020

Sensors

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Nowadays, electrochemical biosensors are reliable analytical tools to determine a broad range of molecular analytes because of their simplicity, affordable cost, and compatibility with multiplexed and point-of-care strategies. There is an increasing demand to improve their sensitivity and selectivity, but also to provide electrochemical biosensors with important attributes such as near real-time and continuous monitoring in complex or denaturing media, or in vivo with minimal intervention to make them even more attractive and suitable for getting into the real world. Modification of biosensors surfaces with antibiofouling reagents, smart coupling with nanomaterials, and the advances experienced by folded-based biosensors have endowed bioelectroanalytical platforms with one or more of such attributes. With this background in mind, this review aims to give an updated and general overview of these technologies as well as to discuss the remarkable achievements arising from the development of electrochemical biosensors free of reagents, washing, or calibration steps, and/or with antifouling properties and the ability to perform continuous, real-time, and even in vivo operation in nearly autonomous way. The challenges to be faced and the next features that these devices may offer to continue impacting in fields closely related with essential aspects of people’s safety and health are also commented upon.

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“…Hence, many scientists are interested in poly-3,4-(crown ether) thiophene (PEDOT) with a short chain. The electrical stability of polythiophene is also increased by co-polymerization, and this leads to an increase in the biosensitive performance of polythiophene-based sensors [4,[17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

A novel lactose biosensor based on electrochemically synthesized 3,4-ethylenedioxythiophene/thiophene (EDOT/Th) copolymer

et al. 2020

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In this study, a new lactose biosensor has been developed in which the 3,4-ethylenedioxythiophene/thiophene (EDOT/Th) copolymer is used as a transducer. The EDOT/Th copolymer was deposited on the glassy carbon electrode to be used as the working electrode. In addition to the working electrode, the three-electrode system was used in both the electrochemical synthesis and in the biosensor measurements. Lactase (β-galactosidase) that catalyzes the breakdown of lactose into monosaccharides (glucose and galactose) and galactose oxidase that catalyzes the oxidation of the resulting galactose were attached to the copolymer by a cross-linker on the modified working electrode. The response of the enzyme electrode to lactose was determined by cyclic voltammetry (CV) at +0.12 V. Enzyme electrode optimization parameters (pH, temperature, enzyme concentration, etc.) were performed. Fourier transform infrared spectroscopy, scanning electron microscopy and CV methods were used to support copolymer formation. In addition, the characteristics of the enzyme electrode prepared in this study (Km, 0.02 mM; activation energy Ea, 38 kJ/mol; linear working range, up to 1.72 mM; limit of detection, 1.9 × 10−5 M and effects of interferents [uric acid and ascorbic acid]) were determined.

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Zwitterionic peptide anchored to conducting polymer PEDOT for the development of antifouling and ultrasensitive electrochemical DNA sensor

Cited by 123 publications

References 28 publications

Antifouling (Bio)materials for Electrochemical (Bio)sensing

Antifouling (Bio)materials for Electrochemical (Bio)sensing

Beyond Sensitive and Selective Electrochemical Biosensors: Towards Continuous, Real-Time, Antibiofouling and Calibration-Free Devices

A novel lactose biosensor based on electrochemically synthesized 3,4-ethylenedioxythiophene/thiophene (EDOT/Th) copolymer

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