Zygosaccharomyces lentus sp. nov., a new member of the yeast genus Zygosaccharomyces Barker

Abstract

Unusual growth characteristics of a spoilage yeast, originally isolated from spoiled whole-orange drink and previously identified as Zygosaccharomyces bailii, prompted careful re-examination of its taxonomic position. Smallsubunit rRNA gene sequences were determined for this strain and for four other strains also originally described as 2. bailii but which, in contrast to other strains of this species, grew poorly or not a t all under aerobic conditions with agitation, failed to grow in the presence of 1 YO acetic acid and failed to grow a t 30 "C. Comparative sequence analysis revealed that these strains represented a phylogenetically distinct taxon closely related to, but distinct from, Z. bailii and Zygosaccharomyces bisponrs. Furthermore, sequence analysis of the internal transcribed spacer (ITS) region showed that, while all five strains had identical ITS2 sequences, they could be subdivided into two groups based on ITS1 sequences. Despite such minor inter-strain sequence variation, these yeasts could readily be distinguished from all other currently described Zygosaccharomyces species by using ITS sequences. On the basis of the phylogenetic results presented, a new species comprising the five strains, Zygosaccharomyces lentus sp. nov., is described and supporting physiological data are discussed, including a demonstration that growth of this species is particularly sensitive to the presence of oxygen. The type strain of Z. lentus is NCYC D2627T. ~~ ~

A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

“…Nine species are currently accepted in the genus (Kurtzman, 1998) and recently two new species, Z. lentus (Steels et al, 1999) and Z. kombuchaensis, have been proposed (Kurtzman et al, 2001;Steels et al, 2002).…”

A novel ascosporogenous yeast species, Zygosaccharomyces siamensis, and the sugar tolerant yeasts associated with raw honey collected in Thailand

“…Accordingly, the genus Kodamaea Yamada, Suzuki, Matsuda et Mikata was proposed for that species (Yamada et al 1995b). In this study, Kodamaea ohmeri also found in A. florea, showed a strong (Kurtzman and Robnett 1998;Kurtzman et al 2001;Rosa and Lachance 2005;Steels et al 1999). Bootstrap values were calculated from 1,000 replicates, and values below 50% were omitted.…”

“…Another related species, Z. mellis is remarkable for extreme osmotolerance and is a spoiling agent of high sugar foods and honey. Physiological characteristics that make Zygosaccharomyces yeasts particularly problematic include their ability to ferment sugar, osmotolerance, resistance to preservatives, formation of heat-resistant ascospores and fructophily (James and Stratford 2003;Steels et al 1999Steels et al , 2002. A previous study (Suezawa et al 2008), using 26S rDNA D1/D2 and ITS sequencing, of the yeasts isolated from miso and soy sauce in Japan revealed two types of Z. mellis.…”

“…Zygosaccharomyces is a yeast genus often associated with food spoilage (James and Stratford 2003;Steels et al 1999). Currently the genus is comprised of 14 species (Kurtzman 2003;Rosa and Lachance 2005;Torriani et al 2011), four of which, Z. bailii, Z. bisporus, Z. lentus and Z. rouxii, are well recognized as the causes of significant amounts of food and beverage spoilage.…”

Zygosaccharomyces kombuchaensis, a new ascosporogenous yeast from 'Kombucha tea'

“…Following autoclaving, the sugared tea was inoculated with pure cultures of the preceding three strains, either singly or in combination, and incubated at room temperature for several days. Stan-dard fermentation and assimilation tests do not allow reliable separation of the new species from the closely related Zygosaccharomyces lentus [7], so a rapid diagnostic method has been developed that allows their recognition from restriction fragment analysis of a region of the rDNA repeat. Kurtzman and Robnett ([6], unpublished data) showed from analyses of large subunit (26S) rDNA domain D1/ D2 sequences that NRRL YB-4810 represented a new species of Zygosaccharomyces and that NRRL YB-4882 is a strain of Pichia £uxuum (anamorph Candida vini).…”