Zygotic Regulation of Maternal Cyclin A1 and B2 mRNAs

Audic, Yann; Anderson, Christina M.; Bhatty, Robert; Hartley, Rebecca S.

doi:10.1128/mcb.21.5.1662-1671.2001

Cited by 31 publications

(69 citation statements)

References 45 publications

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“…The presence of cyclin mRNAs in the polysomal fraction is consistent with the polyadenylation of these mRNAs at this time (compare with 4 hpf embryos in Fig. 2; Sheets et al, 1994) and with the synthesis of cyclin protein (Sheets et al, 1994;Audic et al, 2001). In contrast, in stage 10 embryos (post-MBT), cyclin A1 mRNA is in the nonpolysomal supernatant fraction (S), as is the majority of cyclin B2 mRNA.…”

Section: Release Of Cyclin A1 Mrna From the Polysomes Accompanies Deamentioning

confidence: 52%

“…In contrast, in stage 10 embryos (post-MBT), cyclin A1 mRNA is in the nonpolysomal supernatant fraction (S), as is the majority of cyclin B2 mRNA. Both of these maternal mRNAs are deadenylated by this time (Audic et al, 2001). In contrast, cyclin B1 mRNA retains a poly(A) tail and is still present primarily in the polysomal fraction.…”

Section: Release Of Cyclin A1 Mrna From the Polysomes Accompanies Deamentioning

confidence: 90%

“…least 11 hr in ␣-amanitin-injected embryos, indicating that this mRNA is still translatable (Audic et al, 2001).…”

Section: Deadenylation Of Cyclin A1 Maternal Mrna Requires Zygotic Trmentioning

confidence: 99%

“…We showed previously that the maternal mRNA encoding cyclin A1 is deadenylated beginning approximately 6 hr postfertilization (hpf; Audic et al, 2001). This timing corresponds to the midblastula transition (MBT) when zygotic transcription begins.…”

Section: Deadenylation Of Cyclin A1 Maternal Mrna Requires Zygotic Trmentioning

confidence: 99%

“…We recently reported that cyclin A1 and B2 maternal mRNAs are deadenylated in Xenopus embryos in a manner dependent on zygotic transcription (Audic et al, 2001). Deadenylation precedes the terminal disappearance of maternal cyclin A1 and B2 proteins and the remodeling of the rapid embryonic cell cycle to the longer adult cell cycle.…”

Section: Introductionmentioning

confidence: 99%

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Zygotic control of maternal cyclin A1 translation and mRNA stability

Audic

Garbrecht

Fritz

et al. 2002

Developmental Dynamics

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Cyclin mRNAs are unstable in the adult cell cycle yet are stable during the first 12 cell divisions in Xenopus laevis. We recently reported that cyclin A1 and B2 maternal mRNAs are deadenylated upon completion of the 12th division (Audic et al. [2001] Mol. Cell Biol. 21: 1662-1671). Deadenylation is mediated by the 3 untranslated region (UTR) of the mRNA and precedes the terminal disappearance of the cyclin proteins, with both processes requiring zygotic transcription. The purpose of the current study was (1) to ask whether deadenylation leads to translational repression and/or destabilization of endogenous cyclin A1 and B2 mRNAs, and (2) to further characterize the regulatory sequences required. We show that zygote-driven deadenylation leads to translational repression and mRNA destabilization. A 99-nucleotide region of the 3UTR of the cyclin A1 mRNA mediates both deadenylation and destabilization. Surprisingly, two AU-rich consensus elements within this region are dispensable for this activity. These results suggest that zygote-dependent deadenylation, translational repression, and mRNA destabilization by means of novel 3UTR elements contribute to the disappearance of maternal cyclins. They also suggest that translational control of cyclins may play a role in the transition to the adult cell cycle. These data concur with previous studies in Drosophila showing that zygote-mediated degradation of maternal cdc25 mRNA may be a general mechanism whereby transition to the adult cell cycle proceeds.

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Section: Release Of Cyclin A1 Mrna From the Polysomes Accompanies Deamentioning

confidence: 52%

Section: Release Of Cyclin A1 Mrna From the Polysomes Accompanies Deamentioning

confidence: 90%

“…least 11 hr in ␣-amanitin-injected embryos, indicating that this mRNA is still translatable (Audic et al, 2001).…”

Section: Deadenylation Of Cyclin A1 Maternal Mrna Requires Zygotic Trmentioning

confidence: 99%

Section: Deadenylation Of Cyclin A1 Maternal Mrna Requires Zygotic Trmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Zygotic control of maternal cyclin A1 translation and mRNA stability

Audic

Garbrecht

Fritz

et al. 2002

Developmental Dynamics

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Messenger RNA stockpile of cyclin B, insulin‐like growth factor I, insulin‐like growth factor II, insulin‐like growth factor receptor Ib, and p53 in the rainbow trout oocyte in relation with developmental competence

Aegerter¹,

Jalabert²,

Bobe³

2003

Molecular Reproduction Devel

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In the present study, correlations between the oocyte messenger RNA (mRNA) stockpile of Cyclin B, insulin-like growth factor I (IGF-I), insulin-like growth factor (IGF-II), insulin-like growth factor receptor Ib (IGFR Ib), and p53 transcripts and the developmental competence of the oocyte were studied. For this purpose, post-ovulatory ageing was used as a tool to generate oocytes of varying developmental competence. Mature female rainbow trout were held at 12 degrees C and periodically checked for ovulation. Oocytes were collected from each female at ovulation and 5, 14, 21 days later. For each collected egg batch, the abundance of several mRNAs in the oocyte was analyzed by real-time PCR and embryo development was monitored after fertilization. Egg quality was estimated not only through embryonic survival but also by studying the occurrence of specific morphological abnormalities. The present study showed that oocyte post-ovulatory ageing was associated with variations of the relative abundance of several studied transcripts within the oocyte. In addition, the abundance of specific mRNAs could be correlated with either the embryonic survival or the occurrence of malformations. Thus, the abundance of IGFR Ib and Cyclin B transcripts in the oocyte was correlated with the occurrence of morphological abnormalities observed at yolk-sac resorption (negatively for IGFR Ib and positively for Cyclin B), while the maternal stockpile of IGF-I, IGF-II, and IGFR Ib mRNAs was positively correlated with embryonic survival.

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Gene expression during the oocyte‐to‐embryo transition in mammals

Evsikov¹,

Evsikova²

2009

Molecular Reproduction Devel

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The seminal question in modern developmental biology is the origins of new life arising from the unification of sperm and egg. The roots of this question begin from 19 th -20 th century embryologists studying fertilization and embryogenesis. Although the revolution of molecular biology has yielded significant insight into the complexity of this process, the overall orchestration of genes, molecules, and cells, is still not fully formed. Early mammalian development, specifically the oocyte-to-embryo transition, is essentially under "maternal command" from factors deposited in the cytoplasm during oocyte growth, independent of de novo transcription from the nascent embryo. Many of the advances in understanding this developmental period occurred in tandem with application of new methods and techniques from molecular biology, from protein electrophoresis to sequencing and assemblies of whole genomes. From this bed of knowledge, it appears that precise control of mRNA translation is a key regulator coordinating the molecular and cellular events occurring during oocyte-to-embryo transition. Notably, oocyte transcriptomes share, yet retain some uniqueness, common genetic motifs among all chordates. The common genetic motifs typically define fundamental processes critical for cellular maintenance, whereas the unique genetic features may be a source of variation and a substrate for sexual selection, genetic drift, or gene flow. One purpose for this complex interplay among genes, proteins, and cells may allow for evolution to transform and act upon the underlying processes, at molecular, structural and organismal levels, to increase diversity, which is the ultimate goal of sexual reproduction.

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Zygotic Regulation of Maternal Cyclin A1 and B2 mRNAs

Cited by 31 publications

References 45 publications

Zygotic control of maternal cyclin A1 translation and mRNA stability

Zygotic control of maternal cyclin A1 translation and mRNA stability

Messenger RNA stockpile of cyclin B, insulin‐like growth factor I, insulin‐like growth factor II, insulin‐like growth factor receptor Ib, and p53 in the rainbow trout oocyte in relation with developmental competence

Gene expression during the oocyte‐to‐embryo transition in mammals

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