Zytochemischer Polymorphismus der sauren Phosphatase bei Haarzell-Leukämie

Abstract

In four cases of hairy cell leukemia a cytochemical polymorphism concerning acid phosphatase (AP) is evident. Any AP is lacking in all hairy cells of one case; only tartrate inhibitable AP is occurring in two cases, in another case tartrate resistant AP is found in high activity. Thus, the lack of tartrate resistant AP seems not to be an argument against hairy cell leukemia.

Acid phosphatase and acid esterase activity in neoplastic and non‐neoplastic lymphoid cells A semiquantitative evaluation related to immunological markers in 112 cases

“…Yet, the presence of TrAcP positive cells was by itself not diagnostic of HCL, since some prolymphocytes and B lymphoma cells in the present study displayed this AcP-isoenzyme as well, in agreement with reports from others (25). Cases of HCL completely lacking TrAcP have also been reported (26).…”

Cytochemical Study of Thymocytes and T Lymphocytes

“…The results obtained from the pleural fluid lymphocytes seem to be especially important since these lymphocytes were not subjected to particular trauma in order to obtain a pure T-cell population. The technique of Schaefer et al (1975) was used for determining the presence of the enzyme, because it has the advantage of lending itself to good standardization and reproducibility.…”

“…All the preparations were tested for peroxidase (Graham & Karnovsky, 1966). The presence of AcP (Schaefer et al, 1975), ANAE at pH 5.8 (Semenzato et al, 1979), BG (Lorbacher et al, 1967) and NABG (Hayashi, 1965) were determined by the hexazonium pararosanilin method, while the presence of NBE (Higgy et al, 1977) and NAE at pH 7.4 (Rozenszajn et al, 1978) were determined by means of diazonium salts. Those T lymphocytes that showed an intense positivity of AcP, NAE and NBE in paranuclear zone were considered positive.…”