α-Amylase activity of human neonate and adult saliva

Bellavía, S. L.; Moreno, José; Sanz, Elizabeth; Picas, Elena I.; Blanco, Andrés

doi:10.1016/0003-9969(79)90059-1

Cited by 22 publications

(13 citation statements)

References 13 publications

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“…7). It was recently noted that the salivary amylase locus is the genetic region with the highest degree of large-scale copy number variation in humans, which accords well with the observation that human salivary a-amylase levels are highly variable (Bellavia et al, 1979;Iafrate et al, 2004). Thus, our data raise the possibility that the activity of human salivary a-amylase in combination with diet may underlie the predisposition of certain persons to develop GAS pharyngitis.…”

Section: Discussionsupporting

confidence: 90%

Molecular characterization of group A Streptococcus maltodextrin catabolism and its role in pharyngitis

Shelburne

Keith

Davenport

et al. 2008

Molecular Microbiology

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SummaryWe previously demonstrated that the cell-surface lipoprotein MalE contributes to GAS maltose/ maltodextrin utilization, but MalE inactivation does not completely abrogate GAS catabolism of maltose or maltotriose. Using a genome-wide approach, we identified the GAS phosphotransferase system (PTS) responsible for non-MalE maltose/maltotriose transport. This PTS is encoded by an open reading frame (M5005_spy1692) previously annotated as ptsG based on homology with the glucose PTS in Bacillus subtilis. Genetic inactivation of M5005_spy1692 significantly reduced transport rates of radiolabelled maltose and maltotriose, but not glucose, leading us to propose its reannotation as malT for maltose transporter. The DmalT, DmalE and DmalE:malT strains were significantly attenuated in their growth in human saliva and in their ability to catabolize a-glucans digested by purified human salivary a-amylase. Compared with wild-type, the three isogenic mutant strains were significantly impaired in their ability to colonize the mouse oropharynx. Finally, we discovered that the transcript levels of maltodextrin utilization genes are regulated by competitive binding of the maltose repressor MalR and catabolite control protein A. These data provide novel insights into regulation of the GAS maltodextrin genes and their role in GAS host-pathogen interaction, thereby increasing the understanding of links between nutrient acquisition and virulence in common human pathogens.

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Section: Discussionsupporting

confidence: 90%

Molecular characterization of group A Streptococcus maltodextrin catabolism and its role in pharyngitis

Shelburne

Keith

Davenport

et al. 2008

Molecular Microbiology

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“…The relationship between flow rate and age changes through life span; in newborns the sAA activity is detected very low [48-50] and climbs to the adult levels within the first 3 years [51, 52]. Over the adulthood until older ages the sAA activity seems to remain unchanged [53, 54].…”

Section: Discussionmentioning

confidence: 99%

Salivary Alpha-Amylase Activity and Salivary Flow Rate in Young Adults

Arhakis

Karagiannis²,

Kalfas³

2013

TODENTJ

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The secretion of salivary alpha-amylase (sAA) is more associated with psychoneuroendocrinological response to stress than with the flow rate and age. The aim of this cross sectional study is to build an explanatory model based on patterns of relationship between age 20-39 in resting and stimulated saliva under no stressful condition in healthy volunteers. Both resting and stimulated saliva were collected from 40 subjects. The sAA values were log-transformed, the normality assumption was verified with the Shapiro-Wilk test and the reliability of the measurements was estimated by the Pearsons’ r correlation coefficient. The estimated model was based on the theory of the Linear Mixed Models. Significant mean changes were observed in flow rate and sAA activity between resting and stimulated saliva. The final model consists of two components, the first revealed a positive correlation between age and sAA while the second one revealed a negative correlation between the interaction of age × flow rate in its condition (resting or stimulated saliva), with sAA. Both flow rate and age influence sAA activity.

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“…Amylase (AM): This was determined by Bellavia et al (17) (1979) . The readouts were made at 530nm in a Beckman DU-68 spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Influence of bethanechol on salivary parameters in irradiated patients

et al. 2016

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BackgroundSome studies have shown evidence that the prophylactic use of bethanechol chloride (BC) may be useful in preventing the incidence and/or severity of xerostomia (XT). However, the indication of BC in irradiated patients with XT needs to be better characterized. The study aimed to evaluate the influence of BC on XT, salivary flow rate, and salivary composition in patients previously submitted to head and neck radiotherapy.Material and MethodsForty five irradiated patients complaining of XT used 50 mg/day of BC for 3 months, and the salivary parameters were evaluated in 4 Phases (Before BC therapy, after one month of BC, 2 months of BC, and 3 months of BC). Biochemical analysis included buffering capacity; pH; total protein concentration (TP); amylase concentration (AM); catalase (CAT) and peroxidase (PX) activities. In addition, unstimulated and stimulated salivary flow rates were determined and XT was classified.ResultsAccording to the XT grading system used, patients showed improvement in XT between Phase 1, and Phases 2, 3 and 4. In addition, some changes were observed in TP concentration (decreased); AM concentration (increased); and PX and CAT activities (decreased and increased, respectively) after Phase 2, for stimulated saliva collection (p<0.05).ConclusionsOur results suggested that when BC was used to treat salivary gland dysfunction induced by head and neck radiotherapy, improvement in XT symptoms, and some changes in saliva composition were shown. Key words:Radiotherapy, xerostomia, hyposalivation, saliva, biochemistry.

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α-Amylase activity of human neonate and adult saliva

Cited by 22 publications

References 13 publications

Molecular characterization of group A Streptococcus maltodextrin catabolism and its role in pharyngitis

Molecular characterization of group A Streptococcus maltodextrin catabolism and its role in pharyngitis

Salivary Alpha-Amylase Activity and Salivary Flow Rate in Young Adults

Influence of bethanechol on salivary parameters in irradiated patients

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