α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots

Abstract

<p>Eleven coumarins named osthole (1), imperatorin (2), bergapten (3), prantschimgin (4), grandivitinol (5), suberosin (6), xanthotoxin (7), felamidin (8), marmesin (9), umbelliferone (10), ulopterol (11), and a sterol mixture consisted of stigmasterol (12), β-sitosterol (13) were isolated from the roots of Ferulago blancheana, F. pachyloba and F. trachycarpa through in vitro bioassay-guided fractionation processes. The extracts and bioactive compounds were evaluated for their α-amylase and α-glucosidase activities. Among the tested compounds, felamidin and suberosin showed significant α-glucosidase inhibitory activity with IC<sub>50</sub> values of 0.4 and 0.9 mg/mL, respectively, when compared to the reference standard acarbose (IC<sub>50</sub> = 4.9 mg/mL). Grandivitinol (IC<sub>50</sub> = 20.0 mg/mL) had the lowest inhibitory effect. On the other hand, none of the tested extracts were found to be active on α-amylase inhibition. This is the first report on isolation, characterization of the bioactive compounds and evaluation the α-amylase and α-glucosidase inhibitory activities of these species.</p><p><strong>Video Clip of Methodology</strong>:</p><p>8 min 26 sec: <a href="https://www.youtube.com/v/x0gakPjgXmc">Full Screen</a> <a href="https://www.youtube.com/watch?v=x0gakPjgXmc">Alternate</a></p><p> </p>

Antioxidant, metabolic enzymes inhibitory ability of Torreya grandis kernels, and phytochemical profiling identified by HPLC‐QTOF‐MS/MS

“…This might be speculated that the AGIs present in TGK are more soluble in nonpolar organic solvent. In previous researches, strong α‐glucosidase inhibition of DCF from extracts have been reported in extracts of Ferulago bracteata roots, blancheana , F. pachyloba , and F. trachycarpa (Karakaya, Gözcü, et al, ; Karakaya, Özbek, et al, ).…”

Phytochemical analysis and biological evaluation ofFerulago setifoliaK. Koch

“…3 In traditional medicine, they have been used in carminative, peptic, sedative, aphrodisiac, vermifuge, immunostimulant, flavor, digestive, tonic, germicidal, and antibronchitis applications, as well as against ulceration, nuisance, snakebite, hemorrhoids, and splenetic illnesses. 4 Coumarins, 5,6 sesquiterpenes, 6 strerol, 7,8 and flavonoids 9,10 have previously been found in Ferulago species.…”