α-Amylase production by thermophilic Bacillus coagulans in solid state fermentation

Babu, Kodali Ravindra; Satyanarayana, T.

doi:10.1016/0032-9592(95)87038-5

Cited by 125 publications

(81 citation statements)

References 12 publications

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“…In contrary, yeast extract supplementation in WB is not promising enough in terms of alpha amylase activity (880 ± 13.2 U/g). Earlier results also corroborates with our observation albeit a different school of thought exists [32,33]. Work done by Pandey et al [17] reveals contradictory results indicating no differences in the amylase yield through SSF conducted in WB aided with different N sources [27].…”

Section: Effect Of Extraction Solvent and Nitrogen Sources On The Amysupporting

confidence: 92%

Optimization of Solid State Fermentation Conditions and Characterization of Thermostable Alpha Amylase from Bacillus subtilis (ATCC 6633)

Mallik¹

2015

J Bioprocess Biotech

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Alpha amylase production using microbial source and solid state fermentation has been conducted for past few years in search of thermostable enzyme. Owing to the prolific use of thermostable alpha amylase in various industries like paper, food, detergent, brewing and starch liquefaction process, the production of alpha amylase is still going on. In the present work, Bacillus subtilis (ATCC 6633) has been utilized for generation of alpha amylase followed by optimization of the fermentation media. Change in fermentation conditions like fermentation hour, temperature, inoculums size, nitrogen and sugar sources have pivotal role to enhance alpha amylase yield. The thermal, pH and detergent stability of partially purified alpha amylase have been tested and compared with purified porcine pancreatic amylase. The result is encouraging with approximate 80% retention of alpha amylase activity comparable to purified porcine pancreatic amylase in presence of drastic condition of temperature (60°C), pH (6-11) and detergents. This makes it apt for use in various industries like detergent, food and paper industries.

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Section: Effect Of Extraction Solvent and Nitrogen Sources On The Amysupporting

confidence: 92%

Optimization of Solid State Fermentation Conditions and Characterization of Thermostable Alpha Amylase from Bacillus subtilis (ATCC 6633)

Mallik¹

2015

J Bioprocess Biotech

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“…The use of cost-effective agroresidues as substrates in solid state fermentation may reduce 468 Sanghi, A. et al Purification and characterization of cellulase-free xylanase the cost of enzyme production substantially (37). Solid state fermentation offers several advantages over submerged fermentation including simplicity of media, greater product yield, easier scale up of process, economy of space, no complex machinery, equipment and control systems etc (2,14).…”

Section: Introductionmentioning

confidence: 99%

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Sanghi¹,

Garg²,

Gupta³

et al. 2010

Braz. J. Microbiol.

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The present study describes the one-step purification and characterization of an extracellular cellulase-free xylanase from a newly isolated alkalophilic and moderately thermophilic strain of Bacillus subtilis ASH.Xylanase was purified to homogeneity by 10.5-fold with ~43% recovery using ion-exchange chromatography through CM-Sephadex C-50. The purified enzyme revealed a single band on SDS-PAGE gel with a molecular mass of 23 kDa. It showed an optimum pH at 7.0 and was stable over the pH range 6.0-9.0. The optimum temperature for enzyme activity was 55 °C. The purified xylanase did not lose any activity up to 45 ºC, however, it retained 80% and 51% of its activity after pre-incubation at 55 ºC and 60 ºC, respectively. The enzyme obeyed Michaelis-Menton kinetics towards birch wood xylan with apparent K m 3.33 mg/ml and V max 100 IU/ml. The enzyme was strongly inhibited by Hg 2+ and Cu 2+ while enhanced by Co 2+ and Mn 2+. The purified enzyme could be stored at 4 ºC for six weeks without any loss of catalytic activity. The faster and economical purification of the cellulase-free xylanase from B. subtilis ASH by onestep procedure together with its appreciable stability at high temperature and alkaline pH makes it potentially effective for industrial applications.

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“…Wheat bran was found to be the suitable substrate for α-amylase production when compared to all the other agro-residues. This might be due to the presence of sufficient nutrients and ability to remain loose even under moist conditions, thereby providing a large surface area (Babu and Satyanarayana, 1995). In the subsequent experiments, wheat bran was used as the substrate for the production of α-amylase.…”

Section: Optimization Of Various Physical and Chemical Parameters Formentioning

confidence: 99%

“…Solid-state fermentation dominates over submerged fermentation in aspects such as better yield, simple technique, low capital investment, lower levels of catabolite repression, high stability and better product recovery (Babu and Satyanarayana, 1995). Agro-residues are generally considered as the best substrate for the solid-state fermentation processes (Ellaiah et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Optimization of process parameters for -amylase production under solid-state fermentation by Bacillus Cereus MTCC 10202

Kalaiarasi¹,

Parvatham²

2013

Afr. J. Microbiol. Res.

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An α-amylase producing bacterial strain was isolated from sago factory effluent discharged soil and identified as Bacillus cereus. Production of extracellular α-amylase by B. cereus was studied in solidstate fermentation. Different substrates like wheat bran, maize bran, corn bran, millet bran, rice bran, greengram bran, blackgram bran, cassava peel powder, cotton seed oil cake, coconut oil cake, sesame oil cake and groundnut oil cake were screened to select the suitable substrate for the production of α-amylase. Among the agro-wastes, wheat bran was found to be the best substrate. Various physical and chemical parameters were optimized. Maximum α-amylase yield was achieved at pH 7 with inoculum level of 10% at 50°C and an incubation period of 72 h. The optimum ratio of substrate to moisture level was found to be 1:2 and substrate weight to flask volume was 1:50. Supplementation of starch at 1% (w/w) concentration and yeast extract at 1.5% (w/w) concentration resulted in maximum production of α-amylase. Calcium chloride at a concentration of 1% (w/w) was found to stimulate α-amylase production. Thus, B. cereus produced a high titre of α-amylase in solid state fermentation using inexpensive agroresidue under optimized conditions.

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α-Amylase production by thermophilic Bacillus coagulans in solid state fermentation

Cited by 125 publications

References 12 publications

Optimization of Solid State Fermentation Conditions and Characterization of Thermostable Alpha Amylase from Bacillus subtilis (ATCC 6633)

Optimization of Solid State Fermentation Conditions and Characterization of Thermostable Alpha Amylase from Bacillus subtilis (ATCC 6633)

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Optimization of process parameters for -amylase production under solid-state fermentation by Bacillus Cereus MTCC 10202

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