α-globin-gene switching during the development of chicken embryos: Expression and chromosome structure

Weintraub, Harold; Larsen, A. S.; Groudine, Mark

doi:10.1016/0092-8674(81)90323-8

Cited by 354 publications

(168 citation statements)

References 24 publications

Supporting

Mentioning

155

Contrasting

Unclassified

Order By: Relevance

“…For our discussion it is important to underline that in 10-day chick embryos immature definite red blood cells are released into the blood circulation system where they undergo several divisions and complete their terminal differentiation. These cells express adult alpha-globin genes and both fetal and adult betaglobin genes [Weintraub et al, 1981;Landes et al, 1982]. In adult birds the mature erythroblasts are concentrated in bone marrow and are not released into blood.…”

Section: Discussionmentioning

confidence: 99%

“…In AEVtransformed cultured erythroid cells derived from bone marrow of anemic chickens this region was fully associated with the nuclear matrix both before and after induction of alpha-globin gene transcription. In contrast, in 10-day chick embryo red blood cells (10-day RBC), which actively transcribe globin genes, [Bruns and Ingram, 1973;Weintraub et al, 1981;Singal et al, 2002] the whole 250 Kb genomic region under study was preferentially located outside the nuclear matrix. This related with the apparent absence of RNA polymerase II association with the nuclear matrix.…”

mentioning

confidence: 97%

See 1 more Smart Citation

In embryonic chicken erythrocytes actively transcribed alpha globin genes are not associated with the nuclear matrix

Iarovaia

Borounova

Philonenko

et al. 2008

J of Cellular Biochemistry

View full text Add to dashboard Cite

The spatial organization of a 250 Kb region of chicken chromosome 14, which includes the alpha globin gene cluster, was studied using in situ hybridization of a corresponding BAC probe with nuclear halos. It was found that in non-erythroid cells (DT40) and cultured erythroid cells of definite lineage (HD3) the genomic region under study was partially (DT40 cells) or fully (HD3 cells) associated with the nuclear matrix. In contrast, in embryonic red blood cells (10-day RBC) the same area was located in the crown of DNA loops surrounding the nuclear matrix, although both globin genes and surrounding house-keeping genes were actively transcribed in these cells. This spatial organization was associated with the virtual absence of RNA polymerase II in nuclear matrices prepared from 10-day RBC. In contrast, in HD3 cells a significant portion of RNA polymerase II was present in nuclear matrices. Taken together, these observations suggest that in embryonic erythroid cells transcription does not occur in association with the nuclear matrix.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 97%

In embryonic chicken erythrocytes actively transcribed alpha globin genes are not associated with the nuclear matrix

Iarovaia

Borounova

Philonenko

et al. 2008

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…In addition, It appears to require more than one specific sequence to fully anchor the actin gene to the matrix. Data from other laboratories have shown that there Is usually more than one hypersensitive site surrounding a transcriptionally active gene (22,(54)(55)(56)(57)(58)(59). It might be speculated that some of the proteins responsible for DNAase I hypersensitivity are also responsible for the matrix association of active genes.…”

Section: Resultsmentioning

confidence: 99%

The association of transcribed genes with the nuclear matrix ofDrosophilacells during heat shock

1985

View full text Add to dashboard Cite

ABSIRACTUsing the transcriptional modulation afforded by heat shock, we found that the association of active genes with the nuclear matrix was not dependent on their level of transcription. Heat shock genes were matrix associated both before heat shock (when transcription was relatively low), and during heat shock (when transcription was greatly Increased). Conversely, the cytoplasmic actin gene was matrix associated during normal growth conditions (when transcription was high) and during heat shock (when transcription was greatly decreased). Removal of greater than 99.7% of nascent RNA during preparation of the matrices did not affect these findings. Detailed examination of the cytoplasmic actin gene revealed that Its matrix association was apparently mediated by multiple Interactions near the 5' end of the gene.

show abstract

“…Active genes are undermethylated, particularly in promoter regions, and inactive genes are methylated and present in DNase-resistant chromatin. Thus the inactive, embryonic globin gene is highly methylated in adult hen red blood cells and has lost the DNase sensitivity it showed in the embryo [2]. However, the adult fl-globin gene is DNase-sensitive in the chromatin of embryonic chick red blood cells even though it is not yet active, i.e.…”

Section: Introductionmentioning

confidence: 97%

Methylation of hen erythrocyte DNA

Adams¹,

So²

1989

FEBS Letters

View full text Add to dashboard Cite

We have analysed the 5-methylcytosine content of hen erythrocyte DNA and found it to be lower than that of DNA from other chick tissues analysed. Erythroeyte DNA is also a better substrate for DNA methylase having a five-fold lower Km than DNA from white blood cells, This is probably because it contains a large number of hemimethylated sites.Thus the inverse correlation between methylation and gene expression does not apply to the chick red blood cell.

show abstract

α-globin-gene switching during the development of chicken embryos: Expression and chromosome structure

Cited by 354 publications

References 24 publications

In embryonic chicken erythrocytes actively transcribed alpha globin genes are not associated with the nuclear matrix

In embryonic chicken erythrocytes actively transcribed alpha globin genes are not associated with the nuclear matrix

The association of transcribed genes with the nuclear matrix ofDrosophilacells during heat shock

Methylation of hen erythrocyte DNA

Contact Info

Product

Resources

About