α-Glucosidase Inhibitor from Kothala-himbutu (<i>Salacia reticulata</i> WIGHT)

Ozaki, Sei; Oe, Hiromi; Kitamura, Shinichi

doi:10.1021/np070604h

Cited by 57 publications

(31 citation statements)

References 6 publications

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“…1). 5) To determine the effects of 13-MRT, assessment ofglucosidase inhibition, referring to the method of Toda et al, 6) was carried out. A crude enzyme was prepared from rat intestinal acetone powder (Sigma Aldrich Japan, Tokyo).…”

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confidence: 99%

Hypoglycemic Effect of 13-Membered Ring Thiocyclitol, a Novel α-Glucosidase Inhibitor from Kothala-himbutu (Salacia reticulata)

Oe¹,

Ozaki²

2008

Bioscience, Biotechnology, and Biochemistry

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confidence: 99%

Hypoglycemic Effect of 13-Membered Ring Thiocyclitol, a Novel α-Glucosidase Inhibitor from Kothala-himbutu (Salacia reticulata)

Oe¹,

Ozaki²

2008

Bioscience, Biotechnology, and Biochemistry

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“…Among the several samples tested in the present study, the roots' extract of S. reticulata gave the best score with respect to the contents of 1 and 2. Recently, their desulfonated analogs, neosalacinol [6,10,11] and neokotalanol [6,8,12] have also been reported as another sulfoniums responsible for the activity. Attempts to develop the quantitative analyses of these highly polar constituents for more precise evaluation of Salacia species are in progress.…”

Section: Discussionmentioning

confidence: 99%

Quantitative determination of potent α-glucosidase inhibitors, salacinol and kotalanol, in Salacia species using liquid chromatography–mass spectrometry

Muraoka

Morikawa

Miyake

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

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“…Salacia tends to contain Salacinol, Salaretin, Mangiferin, Kotalanol, Triterpenesand, 13 MRT and Ponkoranol. 10,11 Alpha-glucosidase inhibitors decrease the absorption of carbohydrates from the intestine, resulting in a slower and lower rise in blood glucose throughout the day, especially right after meals. They are important constituents of the traditionally used anti-diabetic medicines, and are also therapeutic for other carbohydrate-metabolic disorders.…”

Section: Recent Preclinical and Clinical Studies Have Demonstrated Thatmentioning

confidence: 99%

Salacia Chinensis Extract (SCE) Modulates Carbohydrates and Lipid Metabolism: in vitro and in vivo Models

Ghanam¹,

Srivastava²,

Deshp³

et al. 2016

EMIJ

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Background: Salacia Chinensis (SC) is herbal medicine in Ayurvedic medicine. The extract of Salacia chinensis showed preventive effects on various metabolic health disorders.Objective: The aim of this study was to elucidate the therapeutic efficacy of the extract of Salacia chinensis extract (SCE) and modulation of carbohydrate and lipid metabolism in in vitro and in vivo models. Methods:In vitro studies were conducted to study the effect of Salacia chinensis extract (SCE, OmniLean TM ) on alpha-glucosidase, pancreatic lipase and HMG-CoA reductase. The activity of α-glucosidase was assessed using mammalian α-glucosidase extracted from rat intestinal acetone powder (Sigma) and p-nitrophenyl L-D glucopyranoside (PNPG) as an artificial substrate. Pancreatic lipase activity was assessed using pancreatic porcine lipase and 4-methylumbelliferyl oleate (MU Oleate) as substrate. Lipase activity was measured using a fluorescence kinetic assay. HMG-CoA reductase was the rate-limiting step in cholesterol synthesis. The activity of HMG-CoA reductase was assessed using HMG-CoA reductase assay kit (Sigma CS1090). Nutrigenomics study was conducted to study the effect of SCE on fatty acids, PPAR gamma and C/EBP α. In an in vivo model, fifty-six (56) male C57BL/6 mice were purchased from Charles River Labs, at the age of 5 weeks. The mice were housed individually in mouse cages in a temperature-controlled room with a 12-hour light and dark cycle and free access to regular rodent chow and water. After a week of adaptation, the mice were divided into 3 groups with 8 mice in each group. All mice were fed a high-fat diet (60% energy from fat) to induce obesity. One of the group was administered with low (100mg/kg/d) and high (500mg/kg/d) doses of SCE suspending in 0.5% carboxymethyl cellulose, respectively for 7.5 weeks (52 days). One group was used as the obese control (N=8) and gavage with the vehicle control. Body weights were obtained daily and food intake was recorded thrice every week. Fecal samples were collected during week 6 and 7. Serum lipid analysis, glycerol, free fatty acids, hormonal assays (ghrelin, adiponectin, leptin, insulin, glucose and GLP), fecal crude lipids and histopathology changes were assessed.Results: Salacia chinensis extract (SCE) showed a significant inhibitory effect on α-glucosidase. SCE demonstrated a significant inhibitory effect against pancreatic lipase, compared to orlistat. SCE inhibited HMG-C0A only at higher concentration (1mg/ml). In nutrigenomics model, SCE upregulated C/EBP alpha and down regulated ACC, PPARG and SCD-1. SCE at the dose of 500mg/kg/d significantly slowed body weight gain, reduced food intake, increased adiponectin, decreased fat pads mass and increased fecal fat excretion in animal model. SCE significantly decreased significantly serum glycerol levels (by 69%) and free fatty acids levels (by 77%) compared to control group. Treatment with SCE decreased by 20% leptin levels and by 21% insulin levels and increases by 16% adiponectin but did not affect glucose or GLP-1...

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α-Glucosidase Inhibitor from Kothala-himbutu (Salacia reticulata WIGHT)

Cited by 57 publications

References 6 publications

Hypoglycemic Effect of 13-Membered Ring Thiocyclitol, a Novel α-Glucosidase Inhibitor from Kothala-himbutu (Salacia reticulata)

Hypoglycemic Effect of 13-Membered Ring Thiocyclitol, a Novel α-Glucosidase Inhibitor from Kothala-himbutu (Salacia reticulata)

Quantitative determination of potent α-glucosidase inhibitors, salacinol and kotalanol, in Salacia species using liquid chromatography–mass spectrometry

Salacia Chinensis Extract (SCE) Modulates Carbohydrates and Lipid Metabolism: in vitro and in vivo Models

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