α-Glucosidase Inhibitory Activity of a 70% Methanol Extract from Ezoishige (<i>Pelvetia babingtonii</i>de Toni) and Its Effect on the Elevation of Blood Glucose Level in Rats

Ohta, Tatsuro; Sasaki, Shigefumi; Oohori, Tadashi; Yoshikawa, Shuji; Kurihara, Hideyuki

doi:10.1271/bbb.66.1552

Cited by 62 publications

(41 citation statements)

References 10 publications

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“…It was reported that 70% of methanolic extracts of Ezoishige showed α-glucosidase inhibitory activity [8]. In this study, the extracts of E. stolonifera showed very weak inhibition on rat small intestinal sucrase, isomaltase and glucoamylase.…”

Section: Discussionmentioning

confidence: 55%

“…According to the glucosidase inhibitory activity of Ezoishige [8], the active compound of E. stolonifera was expected to be polyphenols. In HPLC-PDA analysis of MEE, 18 polyphenolic peaks with lambda max of under 300 nm were detected, and these polyphenols were different from flavonoids.…”

Section: Discussionmentioning

confidence: 99%

“…E. kurome has been reported to have bactericidal and hyaluronidase inhibitory activities [6,7]. Furthermore, glucosidase inhibitory activity was found in Pelveta babingtonii, a seaweed belonging to the order of Fucales [8]. E. stolonifera has also been found to have antioxidant, hepatoprotective and tyrosinase inhibitory activities [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Antidiabetic and Antioxidant Effects of Polyphenols in Brown Alga Ecklonia stolonifera in Genetically Diabetic KK-Ay Mice

Iwai

2008

Plant Foods Hum Nutr

196

113

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The dietary intake and control of blood glucose levels are very important in hyperglycemic patients and alpha-glucosidase inhibitors are a cost-effective means to preventing the progression of diabetes. In search of a natural inhibitor from food materials, alpha-glucosidase inhibitory activity and the anti-hyperglycemic effects of a brown alga, Ecklonia stolonifera, were investigated using non-insulin dependent diabetic mice. Methanolic extract of E. stolonifera (MEE), which contains a high content of polyphenols, showed strong inhibition of alpha-glucosidase in vitro. Male KK-A(y) mice, a genetically non-insulin dependent diabetic model, showed hyperglycemia with aging, but the ingestion of MEE suppressed the increase in plasma glucose and lipid peroxidation levels in unfasted KK-A(y) mice dose dependently. In KK-A(y) mice, which were fed the MEE diet for 4 weeks, MEE moderated the elevation of plasma glucose levels after the oral administration of maltose. The polyphenols in MEE were estimated to be phlorotannins by HPLC-PDA and LC/MS analyses. These results demonstrate that E. stolonifera, seaweed typically used as a health food, has strong antidiabetic and antioxidant effects in vivo, thus, it may have beneficial properties in the prevention of diabetes and could be useful in the development of an antidiabetic pharmaceutical and functional food.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antidiabetic and Antioxidant Effects of Polyphenols in Brown Alga Ecklonia stolonifera in Genetically Diabetic KK-Ay Mice

Iwai

2008

Plant Foods Hum Nutr

196

113

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“…The reaction was initiated by adding a crude ␣-glucosidase solution (0.1 ml) to the reaction mixture; this mixture was then incubated for 60 min at 37˚C, the reaction being terminated by adding 1.0 ml of a 2.0 M maleate-Tris-NaOH buffer (pH 7.4). The glucose released in the reaction mixture was determined by a Glucose C-II Test Wako kit (Wako Pure Chemical Co., Tokyo) （ Ohta et al ., 2002). The significantly difference of each of the two groups was surveyed with a p value by Student's t -test assuming that the dispersion of both the two groups was not equal.…”

Section: Methodsmentioning

confidence: 99%

.ALPHA.-Glucosidase Inhibitory Activity in Leaves of Some Mulberry Varieties

Yatsunami

Saito

Fukuda

et al. 2003

FSTR

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The seasonal and polyploidal changes of ␣-glucosidase (rat-intestinal) inhibitory activity in mulberry leaves were compared in 12 mulberry varieties. Enashi, Fusoumaru, No. 325 and Kenmochi which are classified as 3 major varieties in Japan were harvested in May or August as the field samples. Shimaguwa and the 3 different wild types such as Kuromiguwa, Nagamiguwa, Midoriguwa were harvested in May as the greenhouse samples. Some varieties harvested in the field or greenhouse indicated a difference in ␣-glucosidase. In the field Enashi was strong, while Kenmochi was rather weak. The seasonal changes of inhibition between May and August were differed among the varieties. The 3x variety in the field or greenhouse showed the strong inhibition within the same variety. Keywords: ␣-glucosidase, inhibitor, mulberry, Morus, polyploidy, variety Mulberry leaves (Morus alba L.) have been used as a traditional medicine with anti-inflammatory and anti-hyperglycemic action . The mulberry leaf component such as 1-deoxynojirimycin (DNJ;Yoshikuni, 1988;Asano et al., 1994), 2-arylbenzofuran derivatives (Basnet et al., 1993) and polyhydroxylated alkaloids (Asano et al., 2001) inhibit the activity of ␣-glucosidase of the small intestine, and also prevent sudden hydrolysis of disaccharides, absorption of glucose into the blood vessel, and resultant increase of blood glucose level. DNJ showed stronger inhibitory activity towards rat intestinal maltase and yeast ␣-glucosidase compared with DNJ-trehalamine-fused and -linked compounds (Shiozaki et al., 1998). The extract derived from mulberry leaves potentiated the pilocarpine-induced salivary flow which was correlated with antihyperglycemic effects . Fagomine from mulberry leaves increased the plasma insulin level in STZ-diabetic mice, and this insulin release by fagomine might partly contribute to the antihyperglycemic action (Nojima et al., 1998;Taniguchi et al., 1998). The maximum reduction in blood glucose of leaf extract was ob-served among fruit, trunk bark and root bark (Hosseinzadeh & Sadeghi, 1999).On the other hand, there are many reports concerning artificially polyploidy from the point of view of cytology (Seki & Oshigane, 1953, 1957, 1959Tojyo, 1954Tojyo, , 1963Tojyo, , 1966Das & Krishnaswami, 1964;Das et al., 1970) as mulberry breeding is extremely valuable for the sericulture. It is well accepted that major characteristics of 3, 4x polyploidy are indicated a large leaf, high saccharide content, high yield and high feed efficiency on silkworm among the different polyploidy. We observed that the changes of ␣-glucosidase inhibitory activity in leaves were occurred among mulberry polyploidy. Activity for lowering blood-glucose levels of autumn-rearing silkworm was higher than that of spring-rearing silkworm (Ryu et al., 1997). The changes in ␣-glucosidase inhibitory activity in leaves can be observed in some mulberry varieties, there is no reports concerning of seasonal or varietal changes on the ␣-glucosidase inhibition in leaves of any mulberry variety.In this study the ...

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“…Other have been reported causes lowering of systolic blood pressure also known as hypertensive response and lower the level of total cholesterol, free cholesterol, triglyceride and phospholipids in the liver [52]. A methanolic extract from Pelvetia babingtonii shows an inhibitory effect on α-glucosidase which could make it effective in suppressing postprandial hyperglycemia [100]. Ethanolic extract from Solieria robusta, Iyengaria stellata, Colpomenia si-nuosa, Spatoglossum asperum and Caulerpa racemosa show hypolipidemic activities, as a result shown decrease in the serum total cholesterol, triglyceride and low density lipoprotein cholesterol level in rats [101].…”

Section: Antilipemic Hypocholesterolaemic Activitymentioning

confidence: 99%

Bioactive Compounds and Properties of Seaweeds—A Review

Pal¹,

Kamthania²,

Kumar³

2014

OALib

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In last three decades the discovery of metabolite and biological activities from macroalgae has been increased significantly. Many sophisticated modern research tools are available for making of chemical compound but there are still many natural bioactive compounds in the womb of nature which is a mystery, scientist are still working to find out more biologically active compounds. Bioactive substances from seaweeds currently receive the more attention from the pharmaceutical companies for the drug development as well as the researchers. Seaweeds are taking majority of attention from scientists because of its phenomenon bioactive compounds and its properties like anti-viral, anti-tumor, anti-inflammatory and anti-lipedimic and may more properties. This current review described mainly substances like metabolite, properties, and types of seaweeds. Emphasis is given to main metabolite and properties of seaweeds.

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α-Glucosidase Inhibitory Activity of a 70% Methanol Extract from Ezoishige (Pelvetia babingtoniide Toni) and Its Effect on the Elevation of Blood Glucose Level in Rats

Cited by 62 publications

References 10 publications

Antidiabetic and Antioxidant Effects of Polyphenols in Brown Alga Ecklonia stolonifera in Genetically Diabetic KK-Ay Mice

Antidiabetic and Antioxidant Effects of Polyphenols in Brown Alga Ecklonia stolonifera in Genetically Diabetic KK-Ay Mice

.ALPHA.-Glucosidase Inhibitory Activity in Leaves of Some Mulberry Varieties

Bioactive Compounds and Properties of Seaweeds—A Review

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