α-lipoic acid ameliorates n-3 highly-unsaturated fatty acids induced lipid peroxidation via regulating antioxidant defenses in grass carp ( Ctenopharyngodon idellus )

Dietary nano‐selenium enhances antioxidant capacity and hypoxia tolerance of grass carp Ctenopharyngodon idella fed with high‐fat diet

“…In the antioxidant defence system, the specialized antioxidant enzymes GPX, SOD and CAT play a crucial role in eliminating ROS. MDA is one of the products of lipid peroxidation and is also an important indicator for measuring the oxidative stress (Shi et al, ; Yu et al, ). Se can improve the antioxidant capacity of fish via enhancing the activity of antioxidant enzymes, that is GPX, SOD and CAT (Naderi, Keyvanshokooh, Salati, & Ghaedi, ).…”

“…It has proven to be an effective method of alleviating fish oxidative stress by dietary supplements. For example, dietary α‐lipoic acid ameliorates n‐3 highly unsaturated fatty acids induced lipid peroxidation via regulating antioxidant defences in grass carp C. idella (Shi et al, ). Silymarin supplementation markedly improved antioxidant capacity in grass carp fed with high‐lipid diets (Xiao et al, ).…”

Chemical Analysis of Minor Bioactive Components and Cannabidiolic Acid in Commercial Hemp Seed Oil

“…Concerning the occurrence of MDA in the oils after accelerated photo-oxidation tests, the levels found in assayed samples showed a 1.5-to 2.5-fold increase when compared to the initial values. Photo-oxidation susceptibility of the hemp seed oils may be due to the high content in unsaturated fatty acids recognized as vulnerable by oxygen, especially α-linolenic acid (ALA), that contributes the most to the degree of lipid peroxidation [66]. Pigments present in edible oil are known to play an important role in oxidative stability [67].…”

Effect of dietarySchizochytriumsp. oil as an n‐3 long‐chain polyunsaturated fatty acid source on growth performance, lipid metabolism and antioxidant status in juvenile grass carp (Ctenopharyngodon idellus): A comparative study with fish oil

“…The primer sequences and GenBank accession of National Center for Biotechnology Information (NCBI) for β‐actin, adipose TAG lipase (ATGL), carnitine palmitoyltransferase 1 (CPT‐1), peroxisome proliferator‐activated receptor‐α (PPARα), stearoyl‐CoA desaturase (SCD1), fatty acid synthase (FAS), Acetyl‐CoA carboxylase(ACC), diacylglycerol O‐acyltransferase1 (DGAT1), target of rapamycin (TOR), glutamate dehydrogenase (GLDH) are listed in Table 3 and synthesized by Aoke Dingsheng Biological Technology Co. The RNA extraction, integrity assessment, concentration measurement, reverse transcription and RT‐qPCR were performed as described method previously (Shi et al., 2017). The RT‐qPCR programme concluded an initial activation step at 95°C for 3 min, followed by 38 cycles: 15 s at 95°C and 30 s at 60°C, and 5 s at 65°C.…”

“…The Table 3 and synthesized by Aoke Dingsheng Biological Technology Co. The RNA extraction, integrity assessment, concentration measurement, reverse transcription and RT-qPCR were performed as described method previously (Shi et al, 2017). The RT-qPCR programme concluded an initial activation step at 95°C for 3 min, followed by 38 cycles: 15 s at 95°C and 30 s at 60°C, and 5 s at 65°C.…”