αB-Crystallin is a Cytoplasmic Interaction Partner of the Kidney-Specific Cadherin-16

Thedieck, Cornelia; Kalbacher, Hubert; Kratzer, Ulrich; Lammers, Reiner; Stevanović, Stefan; Klein, Gerd

doi:10.1016/j.jmb.2008.02.008

Cited by 22 publications

(12 citation statements)

References 33 publications

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“…Evidence for a direct interaction between Cdh16 and the cytoskeleton, however, remains elusive in thyroid cells, and the lack of a β-catenin binding site in the cytoplasmic tail of Cdh16 (Wendeler et al, 2004) points to the requirement of a protein linker between Cdh16 and actin filaments. A possible candidate could be αB-crystallin (CRYAB), a small heat-shock protein that interacts with actin filaments, linking them to Cdh16 at the basal side of renal collecting ducts (Thedieck et al, 2008). This chaperone is expressed in the thyroid gland (Calì et al, 2012) and is downregulated in Pax8-depleted cells (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Pax8 controls thyroid follicular polarity through Cadherin-16

Koumarianou

Gómez‐López

Santisteban

2016

Journal of Cell Science

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Organization of epithelial cells during follicular lumen formation is crucial for thyroid morphogenesis and function of the thyroid gland; however, the molecular mechanisms underlying this are poorly understood. To investigate this process, we established three-dimensional (3D) epithelial culture model systems using Fischer rat thyroid (FRT) cells or murine primary thyrocytes that developed polarized spherical structures with a central lumen, mimicking thyroid follicles. Using microarray-based differential expression analysis of FRT cells grown under 2D or 3D conditions, followed by RNA-mediated interference (RNAi) and morphogenetic analysis, we identified a key role for the thyroid transcription factor Pax8 and its target cadherin-16 (Cdh16) in the generation of polarized follicle-like structures. Silencing Pax8 expression inhibited the acquisition of apical–basal membrane polarity and impaired lumen formation. Both laminin and β1-integrin (Itgb1) expression was reduced, and cell cytoskeleton polarized distribution was altered. Silencing Cdh16 expression also led to the formation of defective structures characterized by very low laminin expression at the follicle–matrix interface, downregulation of Itgb1, and unpolarized distribution of cell cytoskeleton. Our results demonstrate that Pax8 controls apical–basal follicular polarization and follicle formation through Cdh16.

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Section: Discussionmentioning

confidence: 99%

Pax8 controls thyroid follicular polarity through Cadherin-16

Koumarianou

Gómez‐López

Santisteban

2016

Journal of Cell Science

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“…Moreover, it also plays a role, at least in the kidney, for maintaining tissue integrity by interacting with Ksp-cadherin-16 and promoting the connection of this polypeptide to the cytoskeleton [132]. In cancer pathologies, HspB5 has been described to promote cell migration and invasion by modulating signal transduction pathways.…”

Section: Hspb1 Hspb5 and Hspb4 Clients And Their Potential Role Imentioning

confidence: 99%

HspB1, HspB5 and HspB4 in Human Cancers: Potent Oncogenic Role of Some of Their Client Proteins

Arrigo

Gibert

2014

Cancers

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Human small heat shock proteins are molecular chaperones that regulate fundamental cellular processes in normal unstressed cells as well as in many cancer cells where they are over-expressed. These proteins are characterized by cell physiology dependent changes in their oligomerization and phosphorylation status. These structural changes allow them to interact with many different client proteins that subsequently display modified activity and/or half-life. Nowdays, the protein interactomes of small Hsps are under intense investigations and will represent, when completed, key parameters to elaborate therapeutic strategies aimed at modulating the functions of these chaperones. Here, we have analyzed the potential pro-cancerous roles of several client proteins that have been described so far to interact with HspB1 (Hsp27) and its close members HspB5 (αB-crystallin) and HspB4 (αA-crystallin).

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“…CDH16 is a member of the cadherin superfamily and is expressed exclusively in the kidney [30] was also detected by RNA-Seq only. The connection of CDH16 to the cytoskeleton is important for maintaining tissue integrity in the kidney, which relies on the interaction of CDH16 with alpha B-crystallin [31]. RhCG is a member of an ammonia transporter family and plays a critical role in ammonium handling and pH homeostasis in the human kidney [32].…”

Section: Discussionmentioning

confidence: 99%

A comparative analysis of tissue gene expression data from high-throughput studies

Ping

Wang

et al. 2012

Chin. Sci. Bull.

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High-throughput technologies were employed over the past decade to study the expression profiles of cells and tissues. There are large collections of accumulated data from public databases and numerous research articles were published on these data. In the current study, we performed meta-analysis on the gene expression data from human liver and kidney tissues produced from five different technologies: EST, SAGE, MPSS, microarray, and RNA-Seq. We found RNA-Seq was the most sensitive in the number of genes it detected while SAGE and MPSS were the least sensitive. For the genes detected by all the platforms, there were generally good correlations to the measured expression levels of corresponding genes. We further compared detected genes to liver/kidney proteomics data from the Human Protein Atlas, and found 960 of the 8764 genes only detected by RNA-Seq were validated by proteomics results. In conclusion, RNA-Seq is a more sensitive and consistent technology compared to the other four high-throughput platforms, though their results are in general agreement. Average coverage was determined to be the preferred measurement to represent gene expression levels by RNA-Seq data and will be used in future works. high-throughput sequencing, tissue transcriptome, comparative analysis Citation:Ping J, Wang Y J, Yu Y, et al. A comparative analysis of tissue gene expression data from high-throughput studies.

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αB-Crystallin is a Cytoplasmic Interaction Partner of the Kidney-Specific Cadherin-16

Cited by 22 publications

References 33 publications

Pax8 controls thyroid follicular polarity through Cadherin-16

Pax8 controls thyroid follicular polarity through Cadherin-16

HspB1, HspB5 and HspB4 in Human Cancers: Potent Oncogenic Role of Some of Their Client Proteins

A comparative analysis of tissue gene expression data from high-throughput studies

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