β-Adrenoceptor distribution in murine lymphoid cell lines

Sympathetic modulation of immunity: Relevance to disease

“…Based on radioligand binding and RT-PCR studies, B cells express a higher density of β-AR than CD4 + Th cells [54,126,129,131,133,[157][158][159]. These receptors are of the β 2 -AR subtype [54,129,131,160] and their stimulation also elevates intracellular concentrations of cAMP [127,131,[135][136][137]148,160].…”

Changes in the TBARs content and superoxide dismutase, catalase and glutathione peroxidase activities in the lymphoid organs and skeletal muscles of adrenodemedullated rats

“…However, during stress the sympathetic nervous system is stimulated and epinephrine is secreted from the adrenal medulla (14). Furthermore, as is the case for all cells responsive to epinephrine via cAMP generation, immune cells display ß-adrenoceptors (15,16). In fact, epinephrine increases the proliferative capacity of CD4+ and CD8+ cells via α-receptors, but inhibits that of CD4+ cells via ß-adrenoceptors (17)(18)(19).…”

Down‐regulation of β‐adrenergic receptors induced by mitogen activation of intracellular signaling events in lymphocytes

“…However, similar responses were found in both cases when stimulation was induced by prostaglandin E~ (PGE]), another hormone that also activates the adenylate cyclase system. These data indicate that the still available receptor sites are functional, differently to what occurs with hyperproliferative cell lines with reduced number of uncoupled fl-adrenergic receptors [10]. This phenomenon would probably indicate the loss of a negative control of lymphocyte proliferation as it was previously demonstrated that proliferation of spleen lymphocytes was inhibited by increasing intracellular cAMP levels [16].…”

“…After 30 min incubation at 30°C samples were filtered through Whatman GF/C filters as indicated before [10] and filters were counted in a Wallac counter. Total binding curves were analyzed by computer program LIGAND as already described [10,11]. The non-specific binding parameter fitted by LIGAN D from the total binding curves did not differ from those determined experimentally using 1 ,uM L-propranolol.…”

“…In fact, several studies have identified and characterized fl-adrenergic receptor (R) on lymphocytes [7] and showed that their activation could exert a control of lymphocyte proliferation by increasing the intracellular levels of cyclic AMP (cAMP) [8,9]. Thus we have previously described the absence of functional fl-adrenoceptors in several hyperproliferative lymphoma cell lines [10,11]. *Corresponding author.…”