β-Carotene, α-tocoferol and rosmarinic acid encapsulated within PLA/PLGA microcarriers by supercritical emulsion extraction: Encapsulation efficiency, drugs shelf-life and antioxidant activity

Giménez-Rota, Carlota; Palazzo, Ida; Scognamiglio, Mariarosa; Mainar, Ana M.; Reverchon, Ernesto; Porta, Giovanna Della

doi:10.1016/j.supflu.2019.01.019

Cited by 45 publications

(25 citation statements)

References 48 publications

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“…Thus, critical aspects concerning the solvent removal after formulation in conventional emulsion method can be avoided [61]. The most recent study using SFEE was published in 2019 by C. Gimenez-Rota et al [113]. In this report, β-carotene is encapsulated together with the anti-oxidants α-tocopherol and rosmarinic acid into PLLA and PLGA particles [113].…”

Section: Drug-loaded Particles Using Supercritical Fluid Emulsion Extmentioning

confidence: 99%

PLA/PLGA-Based Drug Delivery Systems Produced with Supercritical CO2—A Green Future for Particle Formulation?

et al. 2020

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Supercritical carbon dioxide (SC-CO2) can serve as solvent, anti-solvent and solute, among others, in the field of drug delivery applications, e.g., for the formulation of polymeric nanocarriers in combination with different drug molecules. With its tunable properties above critical pressure and temperature, SC-CO2 offers control of the particle size, the particle morphology, and their drug loading. Moreover, the SC-CO2-based techniques overcome the limitations of conventional formulation techniques e.g., post purification steps. One of the widely used polymers for drug delivery systems with excellent mechanical (Tg, crystallinity) and chemical properties (controlled drug release, biodegradability) is poly (lactic acid) (PLA), which is used either as a homopolymer or as a copolymer, such as poly(lactic-co-glycolic) acid (PLGA). Over the last 30 years, extensive research has been conducted to exploit SC-CO2-based processes for the formulation of PLA carriers. This review provides an overview of these research studies, including a brief description of the SC-CO2 processes that are widely exploited for the production of PLA and PLGA-based drug-loaded particles. Finally, recent work shows progress in the development of SC-CO2 techniques for particulate drug delivery systems is discussed in detail. Additionally, future perspectives and limitations of SC-CO2-based techniques in industrial applications are examined.

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Section: Drug-loaded Particles Using Supercritical Fluid Emulsion Extmentioning

confidence: 99%

PLA/PLGA-Based Drug Delivery Systems Produced with Supercritical CO2—A Green Future for Particle Formulation?

et al. 2020

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“…Poly-Lactic-co-Glycolic Acid micro-carriers (PLGA-MCs) were obtained using Supercritical Emulsion Extraction (SEE) technology via dense gas extraction of emulsion oily phase. The process layout is described by a countercurrent packed tower operating in continuous mode (Della Porta et al, 2011;Gimenez-Rota et al, 2019). PLGA-MCs were fabricated using awater-oil-water emulsion (ratio 0.25:5:50).…”

Section: Plga-mcs Fabrication By Supercritical Emulsion Extraction Tementioning

confidence: 99%

Chondrogenic Commitment of human Bone Marrow Mesenchymal Stem Cells cultured under perfusion within a 3D collagen environment releasing hTGF\(\beta\)1

Lamparelli

Lovecchio

Marino

et al. 2020

Preprint

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The optimal growth, maturation and function of bioengineered tissues are mediated by both biochemical and physical cues. We here describe a 3D biomimetic environment directing stem cells towards a chondrogenic phenotype. This system comprises a collagen hydrogel and poly-lactic-co-glycolic acid microcarriers (PLGA-MCs) engineered to protect, carry and release a human Transforming Growth Factor b1 (hTFGb1) payload. PLGA-MCs were prepared using supercritical emulsion extraction technology and integrated into a collagen hydrogel co-seeded with human Bone Marrow Mesenchymal Stem Cells (hBM-MSCs). Testing different concentrations of hTFGb1 supplemented to cell monolayer cultures suggested 10 ng/mL as the most appropriate concentration to promote upregulation of SRY-Related HMG-BOXGene 9 (4-fold) and collagen type II (2-fold) specific markers, at Day 16. A similar growth factor concentration was delivered within the 3D bioengineered environment cultured in a dynamic via a custom perfusion bioreactor. A chondrogenic commitment was obtained as indicated by upregulation of collagen type II (5-fold) and downregulation of collagen types I and III (both 0.1-fold) at Day 16. Histological analysis confirmed the remodeling of the synthetic extracellular matrix in where an enhanced mass exchange was described by FEM analysis of fluid-dynamics and related nutrient mass transfer within the 3D construct. This study supports the use of 3D bioengineered scaffolds cultured in a dynamic environment as a suitable tissue engineered model to study chondrogenic differentiation in vitro and opens perspectives for an injectable collagen-based advanced therapy system.

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“…A valid alternative to conventional micronization techniques is represented by supercritical fluid assisted processes, which are widely used to entrap drugs in polymeric matrices ( Di Capua et al, 2017a , b ; Gimenez-Rota et al, 2019 ; Tirado et al, 2019 ). Ozkan et al (2019) studied the micronization of two flavonoids, quercetin and rutin, and their coprecipitation with polyvinylpyrrolidone (PVP) using the supercritical antisolvent process (SAS).…”

Section: Introductionmentioning

confidence: 99%

Antioxidants entrapment in polycaprolactone microparticles using supercritical assisted injection in a liquid antisolvent

Palazzo

Campardelli

Reverchon

2020

Food and Bioproducts Processing

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β-Carotene, α-tocoferol and rosmarinic acid encapsulated within PLA/PLGA microcarriers by supercritical emulsion extraction: Encapsulation efficiency, drugs shelf-life and antioxidant activity

Cited by 45 publications

References 48 publications

PLA/PLGA-Based Drug Delivery Systems Produced with Supercritical CO2—A Green Future for Particle Formulation?

PLA/PLGA-Based Drug Delivery Systems Produced with Supercritical CO2—A Green Future for Particle Formulation?

Chondrogenic Commitment of human Bone Marrow Mesenchymal Stem Cells cultured under perfusion within a 3D collagen environment releasing hTGF\(\beta\)1

Antioxidants entrapment in polycaprolactone microparticles using supercritical assisted injection in a liquid antisolvent

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