β-Catenin phosphorylation at Y654 and Y142 is crucial for high mobility group box-1 protein-induced pulmonary vascular hyperpermeability

Weng, Jie; Yu, Lei; Chen, Zhen-Feng; Su, Haiying; Yu, Shengxiang; Zhang, Hongjie; Lei, Xiaotian; Chen, Lixian; Cui, Yun; Huang, Qiaobing; Guo, Xiaohua

doi:10.1016/j.yjmcc.2018.12.012

Cited by 12 publications

(9 citation statements)

References 47 publications

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“…When we knocked down β-catenin in endothelial monolayer, the increased TER, tight and adhesin junction proteins and decreased permeability across HUVEC monolayers conferred by terlipressin sharply changed (Figure 6). Whereas p-β-catenin caused adherence junction disruptions and cytoskeleton rearrangement, and then increased vascular endothelial hyperpermeability (Weng, Yu et al, 2019). Therefore, we suggested that activation of the PI3K/β-catenin pathway might be involved in the protective effect of terlipressin on GVB.…”

Section: Discussionmentioning

confidence: 80%

Improvement of gut-vascular barrier by terlipressin reduces bacterial translocation and remote organ injuries in gut-derived sepsis

Chang

Zhang²,

Lin³

et al. 2022

Front. Pharmacol.

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Gut-vascular barrier (GVB) serves as the last barrier to limit the migration of intestinal toxins into the blood circulation. The efficacy of terlipressin (a vasopressin V1 receptor agonist) in reducing GVB and multiple organ damage in gut-derived sepsis is unknown. In this study, we hypothesized that, besides other intestinal barriers, GVB play a key role in gut-derived sepsis and terlipressin improve GVB damage and then reduce bacterial translocation and organ injuries. In vivo, a cecal ligation and puncture mouse model was established. The mice were subjected to examine the damage of GVB determined by intestinal plasmalemma vesicle-associated protein-1(PV-1) and vascular endothelial-cadherin. And the intestinal permeability was assessed by translocation of intestinal bacteria and macromolecules. In vitro, transendothelial electrical resistance (TER) during interleukin (IL)-1β stimulation was measured on endothelial cells with or without small interfering RNA targeting β-catenin (si β-catenin). Terlipressin significantly improved GVB damage and reduced translocation of intestinal macromolecules and bacteria by activating PI3K signaling. Of note, intestinal PV-1 expression was significantly correlated with translocation of macromolecules, and dramatic increase of macromolecules was observed in intestinal tissues whereas fewer macromolecules and bacteria were observed in blood, liver and lung following terlipressin treatment. In vitro, terlipressin restored TER during IL-1β stimulation and si β-catenin transfection blocked the changes delivered by terlipressin. Collectively, terlipressin alleviated GVB damage and subsequent bacterial translocation via blood vessels after sepsis challenge, resulting in reduced distant organ injuries and the responsible mechanisms may involve the activation of PI3K/β-catenin pathway.

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Section: Discussionmentioning

confidence: 80%

Improvement of gut-vascular barrier by terlipressin reduces bacterial translocation and remote organ injuries in gut-derived sepsis

Chang

Zhang²,

Lin³

et al. 2022

Front. Pharmacol.

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“…It has reported that PTK2 could participate in the β-catenin pathway in two ways. First, activated PTK2 could localize at cell junctions and phosphorylate β-catenin Y142 directly 54,55 . Second, PTK2 phosphorylates GSK3 Y279/Y216 to stabilize β-catenin, thereby promoting tumorigenesis 34 .…”

Section: Discussionmentioning

confidence: 99%

“…3G), studies have demonstrated that PTK2 participates in activating β-catenin signaling. On the one hand, PTK2 can directly phosphorylate βcatenin Y142 to promote its translocation into the nucleus 32,33 . On the other hand, it phosphorylates GSK3 Y279/Y216 to promote its ubiquitinated degradation mediated by E3 ligase β-TrCP (β-transducin repeats-containing proteins), thereby stabilizing the expression of total β-catenin 34 (Fig.…”

Section: Nrsn2-as1 Interacts With Ptk2mentioning

confidence: 99%

Long Non-coding RNA NRSN2-AS1 promotes ovarian cancer progression through targeting PTK2/β-catenin pathway

Shen,

Wu,

et al. 2023

Preprint

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As a common malignant tumor among women, ovarian cancer poses a serious threat to their health. This study demonstrates that long non-coding RNA NRSN2-AS1 is over-expressed in ovarian cancer tissues using patient sample and tissue microarrays. In addition, NRSN2-AS1 is shown to promote ovarian cancer cell proliferation and metastasis both in vitro and in vivo. Mechanistically, NRSN2-AS1 stabilizes protein tyrosine kinase 2 (PTK2) to activate the β-catenin pathway via repressing MG-53-mediated ubiquitinated degradation of PTK2, thereby facilitating ovarian cancer progression. Rescue experiments verify the function of the NRSN2-AS1/PTK2/β-catenin axis and the effects of MG53 on this axis in ovarian cancer cells. In conclusion, this study demonstrates the key role of the NRSN2-AS1/PTK2/β-catenin axis for the first time and explores its potential clinical applications in ovarian cancer.

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“…The relationship between Wnt/β-catenin and ALI has been studied in the early stage, among which Wnt5a and Wnt3a play different roles. Therein, β-catenin, as a skeletal protein, plays a role in the stability of VE-cadherin in endothelial cells, and studies have shown that LPS-induced disruption of pulmonary endothelial barrier leads to β-catenin degradation ( Rho et al, 2017 ; Tharakan et al, 2012 ; Weng et al, 2019 ; Zhu et al, 2019 ). Meanwhile, Wnt3a/β-catenin as a classical pathway is involved in maintaining endothelial barrier function, and Wnt3a activation improves ALI( Hii et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

The myosin II inhibitor, blebbistatin, ameliorates pulmonary endothelial barrier dysfunction in acute lung injury induced by LPS via NMMHC IIA/Wnt5a/β-catenin pathway

Zhang

Pan

Zhou

et al. 2022

Toxicology and Applied Pharmacology

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β-Catenin phosphorylation at Y654 and Y142 is crucial for high mobility group box-1 protein-induced pulmonary vascular hyperpermeability

Cited by 12 publications

References 47 publications

Improvement of gut-vascular barrier by terlipressin reduces bacterial translocation and remote organ injuries in gut-derived sepsis

Improvement of gut-vascular barrier by terlipressin reduces bacterial translocation and remote organ injuries in gut-derived sepsis

Long Non-coding RNA NRSN2-AS1 promotes ovarian cancer progression through targeting PTK2/β-catenin pathway

The myosin II inhibitor, blebbistatin, ameliorates pulmonary endothelial barrier dysfunction in acute lung injury induced by LPS via NMMHC IIA/Wnt5a/β-catenin pathway

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