β-Glucosidase catalyzed synthesis of octyl-β-d-glucopyranoside using whole cells of Pichia etchellsii in micro aqueous media

Rather, Mohd Younis; Mishra, Saroj; Chand, Subhash

doi:10.1016/j.jbiotec.2010.09.933

Cited by 29 publications

(23 citation statements)

References 21 publications

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“…For example, almond β‐glucosidase requires a w of 0.4–0.5 for catalyzing an in vivo transglycosylation reaction with short chain alcohols (C3) and a w 0.67–0.8 for higher chain alcohols (C8; Van Rantwijk, Woudenberg‐van Oosterom, & Sheldon, ; Wu, Miao, & Fan, ). Also, the β‐glucosidase of P. etchellsii requires high water activity for generating octyl‐glucoside via a whole‐cell production system (Rather, Mishra, & Chand, ). To that end, we tested the in vitro formation of ethyl‐β‐ d ‐glucoside with different concentrations of ethanol as a glucosyl accepter.…”

Section: Resultsmentioning

confidence: 99%

Direct conversion of cellulose into ethanol and ethyl‐β‐d‐glucoside via engineered Saccharomyces cerevisiae

Jayakody

Liu

Yun

et al. 2018

Biotech & Bioengineering

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Simultaneous saccharification and fermentation (SSF) of cellulose via engineered Saccharomyces cerevisiae is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a biodegradable surfactant, ethyl-β-d-glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT-1) and intracellular β-glucosidase (GH1-1) originating from Neurospora crassa. We identified the formation of ethyl-β-d-glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1-1. The EJ2 strain coproduced 0.34 ± 0.03 g ethanol/g cellulose and 0.06 ± 0.00 g ethyl-β-d-glucoside/g cellulose at a rate of 0.30 ± 0.02 g·L ·h and 0.09 ± 01 g·L ·h , respectively, during the SSF of Avicel PH-101 cellulose, supplemented only with Celluclast 1.5 L. Herein, we report a possible coproduction of a value-added chemical (alkyl-glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1-1 in engineered S. cerevisiae. This coproduction could have a substantial effect on the overall technoeconomic feasibility of theSSF of cellulose.

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Section: Resultsmentioning

confidence: 99%

Direct conversion of cellulose into ethanol and ethyl‐β‐d‐glucoside via engineered Saccharomyces cerevisiae

Jayakody

Liu

Yun

et al. 2018

Biotech & Bioengineering

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“…The optimal water content for the highest 1-hexyl glucoside, 1-octyl glucoside, and 1-decyl glucoside were 10, 15, and 20 % (v/v). Some studies had reported that the water content should be between 4 and 20 % (v/v) depending on the catalyst and the alcohol used to achieve a high yield of alkyl polyglucoside [5,10]. The figure also showed that the APG yield decreased when the water content kept going on.…”

Section: Influence Of Water Contentmentioning

confidence: 98%

Production of Alkyl Polyglucoside Using Pichia pastoris GS115 Displaying Aspergillus aculeatus β-Glucosidase I

Kang

Wei

Guo

et al. 2015

Lecture Notes in Electrical Engineering

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Pichia pastoris GS115 displaying Aspergillus aculeatus β-glucosidase I was used as whole-cell biocatalyst catalyzing the synthesis of 1-hexyl glucoside, 1-octyl glucoside, and 1-decyl glucoside through reverse hydrolysis in water-alcohol two-phase system, while glucose and primary alcohols were used as substrate. In this study, factors affecting the synthetic yield were optimized in our study, including water content, glucose concentration, whole-cell biocatalyst content, the pH value of sodium acetate buffer, and the reaction temperature. In 5 mL reaction volume, the optimal reaction conditions of HG were: 10 % pH 3.0 buffer, 0.2 mol/L glucose, 10 g/L whole-cell biocatalyst; the optimal reaction conditions of DG: 15 % pH 3.0 buffer, 0.6 mol/L glucose, 10 g/L whole-cell biocatalyst; the optimal reaction conditions of DG: 20 % pH 2.0 buffer, 0.2 mol/L glucose, 40 g/L whole-cell biocatalyst; three reactions were conducted under 55°C. After 72 h the maximum yield of HG was 11.5 %, that of OG and DG were 5.3 and 3.2 % after 96 h, respectively. The reutilization experiments inducted that the whole-cell biocatalyst could be used at least for five times without obvious decrease in synthesis ability.

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“…This analysis indicated the presence and accessibility of a variety of a-and b-glucosidase activity with whole cells that can be used in biotransformation activities. Such activities have been reported to be used for synthesis of alkyl a-glucosidases [18], alkyl b-glucosides [19] and gluco-oligosaccharides [20] using different bacterial and yeast systems. Since the objective of the present study was to synthesize GOS, lactose was used as a substrate.…”

Section: Profile Of Hydrolytic Activities Of K Marxianus Ncim 3551mentioning

confidence: 99%

Transgalactosylation of lactose for synthesis of galacto-oligosaccharides using Kluyveromyces marxianus NCIM 3551

2015

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β-Glucosidase catalyzed synthesis of octyl-β-d-glucopyranoside using whole cells of Pichia etchellsii in micro aqueous media

Cited by 29 publications

References 21 publications

Direct conversion of cellulose into ethanol and ethyl‐β‐d‐glucoside via engineered Saccharomyces cerevisiae

Direct conversion of cellulose into ethanol and ethyl‐β‐d‐glucoside via engineered Saccharomyces cerevisiae

Production of Alkyl Polyglucoside Using Pichia pastoris GS115 Displaying Aspergillus aculeatus β-Glucosidase I

Transgalactosylation of lactose for synthesis of galacto-oligosaccharides using Kluyveromyces marxianus NCIM 3551

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