β‐Glucosidase produced by Moniliophthora perniciosa: Characterization and application in the hydrolysis of sugarcane bagasse

Abstract

β-Glucosidases (BGLs) belong to the group of enzymes of cellulases and act in the last stage of cellulose degradation, releasing glucose molecules, eliminating the inhibitory effect of cellobiose. This study focused on the production, characterization, and application of BGL from Moniliophthora perniciosa in the hydrolysis of pretreated sugarcane bagasse (3% NaOH + 6% Na 2 SO 3 ), with varying enzymatic loads and reaction times. The enzyme showed an optimum pH of 4.5 and 60 • C. It was stable at all temperatures analyzed (50-90 • C) and retained about 100% of its activity at 50 • C after 60 min of incubation. Among the ions analyzed, BaCl 2 increased BGL activity 9.04 ± 1.41 times. The maximum production of reducing sugars (89.15%) was achieved after 48 h with 10 mg of protein.

β-Glucosidase and Its Application in Bioconversion of Ginsenosides in Panax ginseng

“…Interestingly, β-glucosidases may also be a cost-effective option to produce biofuels through cellulose saccharification using waste lignocellulosic residues, reducing the accumulation of this material in the natural environment [ 98 , 100 ]. Examples are application of the β-glucosidase produced by Moniliophthora perniciosa can or Anoxybacillus flavithermus subsp.…”

“…Notably, the thermostable β-glucosidase from Aspergillus fumigatus Z5 removes phenolic compounds, so it can be used to degrade polyphenols in lignocellulosic biomass. The thermophilic β-glucosidase from Thermotoga naphthophila RUK-10 was combined with cellulase for the hydrolysis of untreated corn straw, and the conversion rate from cellulose to glucose increased by 93.5% [ 98 , 100 ].…”

Production and Characterization of Carboxymethylcellulase by Submerged Fermentation of <i>Moniliophthora perniciosa</i>

“…However, when we used 0.75 m L -1 of enzymatic extract there was a conversion of 50% after 4 days, and this value remained stable over time (Figure 5). Almeida et al [35] related that the maximum production of reducing sugars (89.15%) was Moniliophthora perniciosa in the hydrolysis of pretreated sugarcane bagasse (3% NaOH + 6% Na2SO3).…”

“…Methods of pretreatments used to depolymerize, solubilize or remove the lignin present in the materials lignocellulolytic, the process of steam explosion followed by alkaline delignification has been one of the most promising for the purification of the three main constituents of biomass in parallel with a significant increase susceptibility of cellulose to enzymatic saccharification [35,36]. By the experiments obtained from the hydrolysis of sugarcane bagasse, it is possible to evaluate the efficiency of substrate show the applicability of the complex, if possible, also obtain efficiency in the characterization of enzymes.…”