β-Hexosaminidase, α-d-mannosidase, and β-mannosidase expression in serum from patients with carbohydrate-deficient glycoprotein syndrome type I

Lysosomal hydrolases in cerebrospinal fluid from subjects with Parkinson's disease

“…A substantially different pattern was found in human sera, due to the appearance of a single peak of activity that could be released by the DEAE-column upon application of a salt gradient, as shown by our group. 9 A similar isoenzymatic pattern has been found in the sera of the PD patients.…”

Fluorimetric determination of activity and isoenzyme composition of N-acetyl-β-D-hexosaminidase in seminal plasma of fertile men and infertile patients with secretory azoospermia

“…This is in agreement with Casal et al (9). Our previous studies (25)(26)(27)(28) of isoenzyme profiles of Hex from various biological sources evaluated by ion exchange chromatography showed that Hex A activity for MUGS is quantitatively lower than that for MUG and is a variable proportion of Hex activity. Some studies have been carried out on the Hex isoenzyme pattern in seminal plasma.…”

Glycosidases in the plasma membrane of Ceratitis capitata spermatozoa

“…In fact, the affinity toward 4MU-GlcNAc is about 5-fold higher than the affinity toward 4MU-GalNAc. Activity toward negatively charged substrate has not been detected, suggesting the absence of the a-subunit that is however present in D. melanogaster (Cattaneo et al, 2002), in somatic cells of nematode Trichinella spiralis (Costanzi et al, 1997) and in mammals, including man (Kytzia and Sandhoff, 1985;Beccari et al, 2000;Mencarelli et al, 2005). These findings and the presence of a protein band of 73 kDa on SDS-PAGE suggest that Ceratitis b-Nacetylhexosaminidase is very similar to the D. melanogaster HEXB (DmHEXB) (Cattaneo et al, 2002(Cattaneo et al, , 2006.…”