β-Nerve Growth Factor Levels in Newborn Cord Sera

Abstract

ABSTRACT. This study was designed to examine @-nerve ment and maintenance of sympathic and neural crest-derived growth factor (NGF) levels in human cord blood by a two-sensory innervation in the periphery and of cholinergic innersite enzyme immunoassay using MAb 27/21 to mouse NGF vation in the CNS (3, 4). and to determine whether @-NGF levels show develop-A fruitful method in NGF research has been the introduction mental changes. Blood was collected at delivery from 61 of a sensitive two-site EIA with MAb 27/21 to mouse @-NGF newborns, 55 neonates appropriate for gestational age (46 that allows reliable determination of low levels of 0-NGF present term infants and 9 premature infants), 5 neonates small for in peripheral and CNS tissue (5, 6). Recently, the two-site EIA gestational age, and 1 neonate with congenital hydroceph-was applied to human adult sera and showed low levels of /3-alus. In addition, samples were collected from 2 microce-NGF (0.4 f 0.1 ng/mL) (7). The specific objectives of this study phalic children (microcephaly vera) aged 15 and 18 mo, 2 were to examine levels of P-NGF in human cord blood using the control children, and 4 healthy adults. Mean levels of NGF two-site EIA with the MAb 2712 1 to mouse (3-NGF and to in preterm infants ( n = 9; 13.7 f 8 pg/mL) were signifi-determine whether levels of 8-NGF show developmental cantly lower than levels in term infants ( n = 47; 21.2 f changes, as has been reported for P-NGF in animals (3). During 8.8 pg/mL; p = 0.034 by Mann-Whitney U test). There the course of this study, we were also interested in measuring /3-was no correlation between birth weight, length, head NGF levels in two children with cerebral malformation (microcircumference, and @-NGF levels. In microcephalic chil-cephaly vera) and comparing their results with those found in dren, NGF levels were low (8 pg/mL) compared with the neonatal period and in otherwise healthy children and adults. control infants' values (22 pg/mL). In adults, 8-NGF levels were higher and ranged between 238 and 292 pg/mL. Our MATERIALS AND METHODS study demonstrates that @-NGF levels can be assessed in human newborn sera using a two-site enzyme immunoassay Subjects. Between October and December 1992, levels of Pwith MAb 27/21 to mouse @-NGF, that @-NGF levels are NGF were measured from cord blood in 6 1 newborn infants. As extremely low in newborns compared with adults, that j3-emphasized above, we included 2 microcephalic children, 2 NGF levels seems to show developmental changes, and healthy children considered controls, and 4 healthy adults after that @-NGF levels may be used to assess NGF utilization obtaining parental and adult consent. This study was approved under normal and pathologic conditions such as cerebral by our institutional committee for ethics. malformations. (Pediatr Res 35: 637-639, 1994) Blood samples and pretreatment. Immediately after birth and after clamping the cord, placenta and cord were removed. Then Abbreviations 2.5 mL of blood were collected by cord puncture. In children and a...

Reduced maternal and cord nerve growth factor levels in preterm deliveries

“…NGF in the amniotic fluid has been reported to increase with gestational age (Marx et al, 1999). Reduced levels of NGF also been studied in smaller size preterm infants ( n = 9) (Haddad et al, 1994). However there are no studies which have simultaneously examined levels of NGF in cord as well as in the mother.…”

“…Inspite of the importance of NGF in neurodevelopment, there are limited studies examining the role of plasma NGF both in mothers and infants. The few studies that have been carried out are on a small sample size, only on infants born preterm (Haddad et al, 1994), in the amniotic fluid (Marx et al, 1999) or in intrauterine growth restricted (IUGR) fetuses and neonates (Malamitsi‐Puchner et al, 2007).…”

Nerve growth factor, birth outcome and pre‐eclampsia

“…In this study, though the neonates from both NT‐LBW and PE‐LBW groups had similar infant size, cord NGF levels are significantly lower in PE‐LBW group as compared to NT‐LBW group suggesting that LBW babies born to preeclamptic women may be at higher risk for neurodevelopment disorders in their later life. NGF levels are known to be associated with developmental changes and may also be used to assess NGF utilization under normal and pathologic conditions such as cerebral malformations (Haddad et al, 1994).…”

Changes in Neurotrophin Levels in Umbilical Cord Blood From Infants With Different Gestational Ages and Clinical Conditions

“…Circulating BDNF levels correlate with cortical BDNF levels in newborn rats (18). Nerve growth factor levels measured in umbilical cord blood were higher in samples from infants born at term compared with those obtained from preterm infants (19). There are no available data described about changes in trends of BDNF and NT3 levels with increasing gestational age or after birth in humans.…”