β-Oxidation of fatty acids by <i>Leptospira</i>

Henneberry, Richard C.; Cox, Charles D.

doi:10.1139/m70-007

Cited by 39 publications

(18 citation statements)

References 12 publications

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“…Beta-oxidation of long-chain fatty acids serves as the major mechanism for energy and carbon acquisition by Leptospira [33,34,75,76]. The gene encoding a predicted enoyl-CoA hydratase (LIC12629), which catalyzes the second step of fatty acid oxidation [77], was up-regulated in response to serum, but the expression of other genes in the fatty acid oxidation pathway was not altered.…”

Section: Resultsmentioning

confidence: 99%

Global transcriptomic response of Leptospira interrogans serovar Copenhageni upon exposure to serum

Patarakul

Adler

2010

BMC Microbiol

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BackgroundLeptospirosis is a zoonosis of worldwide distribution caused by infection with pathogenic serovars of Leptospira spp. The most common species, L. interrogans, can survive in the environment for lengthy periods of time in between infection of mammalian hosts. Transmission of pathogenic Leptospira to humans mostly occurs through abraded skin or mucosal surfaces after direct or indirect contact with infected animals or contaminated soil or water. The spirochete then spreads hematogenously, resulting in multi-organ failure and death in severe cases. Previous DNA microarray studies have identified differentially expressed genes required for adaptation to temperature and osmolarity conditions inside the host compared to those of the environment.ResultsIn order to identify genes involved in survival in the early spirochetemic phase of infection, we performed a transcriptional analysis of L. interrogans serovar Copenhageni upon exposure to serum in comparison with EMJH medium. One hundred and sixty-eight genes were found to be differentially expressed, of which 55 were up-regulated and 113 were down-regulated. Genes of known or predicted function accounted for 54.5 and 45.1% of up- and down-regulated genes, respectively. Most of the differentially expressed genes were predicted to be involved in transcriptional regulation, translational process, two-component signal transduction systems, cell or membrane biogenesis, and metabolic pathways.ConclusionsOur study showed global transcriptional changes of pathogenic Leptospira upon exposure to serum, representing a specific host environmental cue present in the bloodstream. The presence of serum led to a distinct pattern of gene expression in comparison to those of previous single-stimulus microarray studies on the effect of temperature and osmolarity upshift. The results provide insights into the pathogenesis of leptospirosis during the early bacteremic phase of infection.

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Section: Resultsmentioning

confidence: 99%

Global transcriptomic response of Leptospira interrogans serovar Copenhageni upon exposure to serum

Patarakul

Adler

2010

BMC Microbiol

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“…Its oligonucleotide pattern is distinct and has an SAB value of <0.2 (22). Leptospira biflexa has a unique metabolism which uses fatty acids or alcohols as its sole source of carbon and energy (12). Its distinct mode of motility allows it to both swim freely in liquid media and move in a corkscrew-like manner through semisolid medium (2,7).…”

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confidence: 99%

Identification and nucleotide sequence of the Leptospira biflexa serovar patoc trpE and trpG genes

Yelton

Peng

1989

J Bacteriol

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“…are thin, motile, spiral-shaped, aerobic bacteria which use long-chain fatty acids or alcohols as their sole carbon and energy sources (16,19). Only a few studies have been done on the physiology of Leptospira (2,6,14,19,30). The results of these studies suggest that, with the exception of isoleucine, which can be made by either of two pathways, Leptospira synthesizes its amino acids by the usual pathways (6,30).…”

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Analysis of cloned DNA from Leptospira biflexa serovar patoc which complements a deletion of the Escherichia coli trpE gene

Yelton¹,

Cohen²

1986

J Bacteriol

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To analyze the cloned region of the chromosome of the spirochete Leptospira biflexa serovar patoc which complemented a defect in the trpE gene of Escherichia coli, we performed a series of experiments involving subcloning, transposon mutagenesis, and maxicells. By subcloning into pBR322 we were able to isolate the Leptospira genes on a 9.7-kilobase pair plasmid (pYC6). Transposon mutagenesis with Tn5 identified a 2.8-kilobase pair region of this plasmid as being necessary to complement a trpE deletion mutation in E. coli. Transformation of plasmid pYC6 into E. coli cells deleted for trpE and the proximal end of trpD showed that the Leptospira DNA complemented both defects. A maxicell analysis of various transposon-induced mutations of the plasmid revealed that three proteins (53.5, 23.6, and 22 kilodaltons) were encoded by the 2.8-kilobase pair region of the Leptospira genome. Two different promoters controlled the production of these three proteins.

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β-Oxidation of fatty acids by Leptospira

Cited by 39 publications

References 12 publications

Global transcriptomic response of Leptospira interrogans serovar Copenhageni upon exposure to serum

Global transcriptomic response of Leptospira interrogans serovar Copenhageni upon exposure to serum

Identification and nucleotide sequence of the Leptospira biflexa serovar patoc trpE and trpG genes

Analysis of cloned DNA from Leptospira biflexa serovar patoc which complements a deletion of the Escherichia coli trpE gene

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