2018
DOI: 10.1038/s41598-018-20311-6
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β-Sitosterol targets Trx/Trx1 reductase to induce apoptosis in A549 cells via ROS mediated mitochondrial dysregulation and p53 activation

Abstract: β-Sitosterol (BS), a major bioactive constituent present in plants and vegetables has shown potent anticancer effect against many human cancer cells, but the underlying mechanism remain elusive on NSCLC cancers. We found that BS significantly inhibited the growth of A549 cells without harming normal human lung and PBMC cells. Further, BS treatment triggered apoptosis via ROS mediated mitochondrial dysregulation as evidenced by caspase-3 & 9 activation, Annexin-V/PI positive cells, PARP inactivation, loss of MM… Show more

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Cited by 90 publications
(73 citation statements)
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“…1 HNMR and 13 CNMR in Table 1 and 12.15, 19.09, 19.57, 20.19, 19.42, 12.26, indicating six methyl groups (-CH 3 ). 13 CNMR, DEPT have resolved six methyls (-CH 3 ), 11 methylene (CH 2 ), nine methines (CH), three quaternary (C) and six glucose moiety carbon [5 methine carbon (CH) and one methylene carbon (CH 2 )] which were also confirmed by HSQC spectra. Cross-peaks in the COSY spectrum shown in Fig.…”
mentioning
confidence: 75%
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“…1 HNMR and 13 CNMR in Table 1 and 12.15, 19.09, 19.57, 20.19, 19.42, 12.26, indicating six methyl groups (-CH 3 ). 13 CNMR, DEPT have resolved six methyls (-CH 3 ), 11 methylene (CH 2 ), nine methines (CH), three quaternary (C) and six glucose moiety carbon [5 methine carbon (CH) and one methylene carbon (CH 2 )] which were also confirmed by HSQC spectra. Cross-peaks in the COSY spectrum shown in Fig.…”
mentioning
confidence: 75%
“…IR spectra were obtained in potassium bromide pellets on a Perkin Elmer spectrometer. 1 HNMR and 13 CNMR spectra of compound (1) were acquired on a Bruker Avance II spectrometer, operating at 400 MHz for 1 HNMR and 100 MHz for 13 CNMR in CDCl3 solvent. 1DNMR ( 1 HNMR and 13 CNMR), and 2D NMR (HSQC, COSY and HMBC) spectra of compound (2) were recorded in DMSO-d6 solvent on a Bruker Avance Neo 500 MHz NMR spectrometer, operating 500 MHz for 1 HNMR and 125 MHz for 13 CNMR with TMS as the internal standard.…”
Section: Materials and Methods: Collection And Identification Of Planmentioning
confidence: 99%
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“…Cancer cell lines through which β-sitosterol exerts its anticancer activities include, breast, colon, lung, gastric, and prostate cancers [ 45 ]. In A549 cells, β-sitosterol target the enzyme Trx/Trx1 reductase to induce apoptosis through ROS and p53 activation [ 46 ]. β-sitosterol anticancer activities have been explored and induce endoreduplication in HL60 and U937 cell lines via PI3K/Akt and Bcl-2 pathways.…”
Section: Mechanisms Of Plant-derived Agents Induced Cell Deathmentioning
confidence: 99%