β1 integrin–mediated signaling induces intercellular adhesion molecule 1 and Fas on rheumatoid synovial cells and Fas‐mediated apoptosis

Nakayamada, Shingo; Saito, Kazuyoshi; Fujii, Koichi; Yasuda, Manabu; Tamura, Masahito; Tanaka, Yoshiya

doi:10.1002/art.10941

Cited by 32 publications

(30 citation statements)

References 38 publications

(37 reference statements)

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“…Consistent with the literature (10,11,46), ␣ v ␤ 3 , ␣ v ␤ 5 , and ␣ v integrins were expressed in RA FLS as were ␣ 3 and ␤ 1 (Fig. 5A).…”

Section: Surface Expression and Role Of Integrin Receptors In Ad5gfp-supporting

confidence: 91%

“…The difference between transduction efficiency of these two vectors in RA FLS and synovial tissue may be explained by different integrin expression in FLS compared with cellular infiltrates in human or mouse synovium. RA FLS and tissue are known to express multiple integrins (10). Among these, RGD-binding integrins such as ␣ v ␤ 3 , ␣ v ␤ 5 , ␣ 5 ␤ 1 , non-RGD-binding integrins such as ␣ 3 ␤ 1 , and LDV-binding integrins ␣ 4 ␤ 1 have been described (10,12,46).…”

Section: Discussionmentioning

confidence: 99%

“…RA FLS and tissue are known to express multiple integrins (10). Among these, RGD-binding integrins such as ␣ v ␤ 3 , ␣ v ␤ 5 , ␣ 5 ␤ 1 , non-RGD-binding integrins such as ␣ 3 ␤ 1 , and LDV-binding integrins ␣ 4 ␤ 1 have been described (10,12,46). In murine synovial cells and tissue, CAR is also notably absent, and ␣ v ␤ 3 and ␣ v ␤ 5 integrins are expressed (13).…”

Section: Discussionmentioning

confidence: 99%

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Enhancement of Adenovirus-Mediated Gene Delivery to Rheumatoid Arthritis Synoviocytes and Synovium by Fiber Modifications: Role of Arginine-Glycine-Aspartic Acid (RGD)- and Non-RGD-Binding Integrins

Toh

Hong

Loo

et al. 2005

The Journal of Immunology

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Rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) do not express the coxsackie-adenovirus (Ad) receptor and are poorly permissive to Ad serotype 5 (Ad5). Genetically modified, coxsackie-Ad receptor-independent Ad5 vectors were studied for gene delivery in human RA FLS and synovium explants and murine collagen-induced arthritis. Short-fiber Ad5 vectors with seven fiber shaft repeats Ad5GFP-R7-knob, Ad5GFP-R7-arginine-glycine-aspartic acid (RGD) (RGD-liganded), and Ad5GFPΔknob (knob-deleted) were compared with Ad5GFP-FiWT, a conventional wild-type (WT) Ad5 vector. Gene transfer by Ad5GFP-R7-knob and Ad5GFP-R7-RGD was 40- to 50-fold and 25-fold higher, respectively, than Ad5GFP-FiWT in FLS. Ad5GFPΔknob was more efficacious than its knob-bearing version Ad5GFP-R7-knob in FLS transduction. Virus attachment and entry required RGD- and LDV-binding integrins including αv, αvβ3, avβ5, and β1. Ad5GFP-R7-knob infection of FLS was partially neutralized by synovial fluid (SF), but remained 30- to 40-fold higher than Ad5GFP-FiWT in the presence of SF. Ad5GFPΔknob was partially neutralized by SF at low virus input, but escaped viral neutralization by SF at higher virus input. Gene transfer to human synovium ex vivo explants and murine collagen-induced arthritis in vivo was also more efficient with short fiber-modified vectors (with and without the knob domain) than Ad5GFPFiWT. Gene transfer by short fiber-modified vectors was enhanced by inflammatory cytokines in vitro and in the presence of inflammation in murine synovium in vivo. Our data indicated that the highly efficient gene delivery RA was mediated by RGD- and non-RGD-binding integrins and enhanced by inflammation. Short fiber modifications with knob ablation may be a strategy to enhance gene delivery, reducing vector dose and vector-induced inflammation and toxicity.

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“…Consistent with the literature (10,11,46), ␣ v ␤ 3 , ␣ v ␤ 5 , and ␣ v integrins were expressed in RA FLS as were ␣ 3 and ␤ 1 (Fig. 5A).…”

Section: Surface Expression and Role Of Integrin Receptors In Ad5gfp-supporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of Adenovirus-Mediated Gene Delivery to Rheumatoid Arthritis Synoviocytes and Synovium by Fiber Modifications: Role of Arginine-Glycine-Aspartic Acid (RGD)- and Non-RGD-Binding Integrins

Toh

Hong

Loo

et al. 2005

The Journal of Immunology

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“…Binding of OPN to these surface receptors is known to promote cell adhesion and migration and specific signaling events of the host cells. Although a few reports have suggested that some integrins were expressed on RA ST (18)(19)(20), we believe that this is the first report comparing the levels of coexpression of OPN receptors in rheumatoid synovial T cells with levels of OPN expression. The observed correlation supports the notion that these receptors are preferentially involved in molecular interactions with OPN in rheumatoid synovium.…”

Section: Figurementioning

confidence: 71%

Role of osteopontin in amplification and perpetuation of rheumatoid synovitis

Nie²,

Li³

et al. 2005

J. Clin. Invest.

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Osteopontin (OPN) is an extracellular matrix protein of pleiotropic properties and has been recently recognized as a potential inflammatory cytokine. In this study, we demonstrate, for the first time to our knowledge, that overexpression of OPN in synovial T cells is associated with local inflammatory milieu and that OPN acts as an important mediator in amplification and perpetuation of rheumatoid synovitis. The study revealed that mRNA expression of OPN was highly elevated in CD4 + synovial T cells derived from patients with RA, which correlated with increased OPN concentrations in synovial fluid (SF). The pattern of OPN overexpression was confined to rheumatoid synovium and correlated with coexpression of selected OPN receptors in synovial T cells, including integrins αv and β1 and CD44. RA-derived SF stimulated the expression of OPN in T cells, which was attributable to IL-10 present in SF and abrogated by anti-IL-10 antibody. Among the more than 300 autoimmune and inflammatory response genes examined, OPN selectively induced the expression of proinflammatory cytokines and chemokines known to promote migration and recruitment of inflammatory cells. Furthermore, it was evident that OPN activated transcription factor NF-κB in mononuclear cells. The study has important implications for understanding the role of OPN in rheumatoid synovitis and other inflammatory conditions.

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“…It recruits leukocytes to the inflammation site by cell-cell interactions and leukocyte extravasation through the vascular endothelium. ICAM-1 expression is significantly increased at inflammation sites, and involvement of this molecule in the pathogenesis of inflammatory diseases like rheumatoid arthritis has been implicated (34). Leukocytes, including monocytes, play an important role in the inflammatory responses.…”

Section: Vol 73 2005 Induction Of Icam-1 and Cytokines By Spirochetmentioning

confidence: 99%

Upregulation of Intercellular Adhesion Molecule 1 and Proinflammatory Cytokines by the Major Surface Proteins of Treponema maltophilum and Treponema lecithinolyticum , the Phylogenetic Group IV Oral Spirochetes Associated with Periodontitis and Endodontic Infections

Lee¹,

Kim

Choi

2005

Infect Immun

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Treponema maltophilum and Treponema lecithinolyticum belong to the group IV oral spirochetes and are associated with endodontic infections, as well as periodontitis. Recently, the genes encoding the major surface proteins (Msps) of these bacteria (MspA and MspTL, respectively) were cloned and sequenced. The amino acid sequences of these proteins showed significant similarity. In this study we analyzed the functional role of these homologous proteins in human monocytic THP-1 cells and primary cultured periodontal ligament (

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β1 integrin–mediated signaling induces intercellular adhesion molecule 1 and Fas on rheumatoid synovial cells and Fas‐mediated apoptosis

Cited by 32 publications

References 38 publications

Enhancement of Adenovirus-Mediated Gene Delivery to Rheumatoid Arthritis Synoviocytes and Synovium by Fiber Modifications: Role of Arginine-Glycine-Aspartic Acid (RGD)- and Non-RGD-Binding Integrins

Enhancement of Adenovirus-Mediated Gene Delivery to Rheumatoid Arthritis Synoviocytes and Synovium by Fiber Modifications: Role of Arginine-Glycine-Aspartic Acid (RGD)- and Non-RGD-Binding Integrins

Role of osteopontin in amplification and perpetuation of rheumatoid synovitis

Upregulation of Intercellular Adhesion Molecule 1 and Proinflammatory Cytokines by the Major Surface Proteins of Treponema maltophilum and Treponema lecithinolyticum , the Phylogenetic Group IV Oral Spirochetes Associated with Periodontitis and Endodontic Infections

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