β1-integrin via NF-κB signaling is essential for acquisition of invasiveness in a model of radiation treated in situ breast cancer

Nam, Jwa Min; Ahmed, Kazi Mokim; Costes, Sylvain V.; Zhang, Hui; Onodera, Yasuhito; Olshen, Adam B.; Hatanaka, Kanako C.; Kinoshita, Rumiko; Ishikawa, Masayori; Sabe, Hisataka; Shirato, Hiroki; Park, Catherine C.

doi:10.1186/bcr3454

Cited by 34 publications

(39 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, recent studies showed that several integrins ( i.e. Itga2, Itga5, Itgb1, and Itgb4) are NFκB regulated (64–69). These findings suggest that integrin-based cellular function ( i.e.…”

Section: Discussionmentioning

confidence: 99%

Molecular Signatures of Membrane Protein Complexes Underlying Muscular Dystrophy

Turk

Hsiao

Smits

et al. 2016

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Mutations in genes encoding components of the sarcolemmal dystrophin-glycoprotein complex (DGC) are responsible for a large number of muscular dystrophies. As such, molecular dissection of the DGC is expected to both reveal pathological mechanisms, and provides a biological framework for validating new DGC components. Establishment of the molecular composition of plasma-membrane protein complexes has been hampered by a lack of suitable biochemical approaches. Here we present an analytical workflow based upon the principles of protein correlation profiling that has enabled us to model the molecular composition of the DGC in mouse skeletal muscle. We also report our analysis of protein complexes in mice harboring mutations in DGC components. Bioinformatic analyses suggested that cell-adhesion pathways were under the transcriptional control of NFκB in DGC mutant mice, which is a finding that is supported by previous studies that showed NFκB-regulated pathways underlie the pathophysiology of DGC-related muscular dystrophies. Moreover, the bioinformatic analyses suggested that inflammatory and compensatory mechanisms were activated in skeletal muscle of DGC mutant mice. Additionally, this proteomic study provides a molecular framework to refine our understanding of the DGC, identification of protein biomarkers of neuromuscular disease, and pharmacological interrogation of the DGC in adult skeletal muscle https://www.mda.org/disease/congenital-muscular-dystrophy/research.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Signatures of Membrane Protein Complexes Underlying Muscular Dystrophy

Turk

Hsiao

Smits

et al. 2016

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

show abstract

“…36 Other authors showed that malignant phenotype emerged in a breast cancer model, characterized by upregulation of MMP-9, among other molecules, and loss of E-cadherin. 49 A noninvasive human lung tumor cells study demonstrated that E-cadherin upregulates MMP-2 and MMP-9 expressions at the protein level. 32 Those studies let us to think the positive correlation found in our results can be a coincidence biased by small sample size.…”

Section: Discussionmentioning

confidence: 99%

Matrix Metalloproteinases 2 and 9 and E-Cadherin Expression in the Endometrium During the Implantation Window of Infertile Women Before In Vitro Fertilization Treatment

Maia-Filho

Rocha²,

Ferreira

et al. 2015

Reprod. Sci.

View full text Add to dashboard Cite

Objective: To evaluate the expression of endometrial matrix metalloproteinases (MMPs) 2 and 9 and E-cadherin in periimplantation phase of infertile women who have undergone in vitro fertilization (IVF) cycles. Methods: This prospective study included 51 patients who underwent endometrial biopsy during the receptive phase in a menstrual cycle prior to IVF treatment. The samples were evaluated by tissue microarray for immunohistochemical study. Results: The expression of MMP-2, MMP-9, and E-cadherin in the endometrium prior to IVF treatment was not associated with pregnancy. There was a decrease in E-cadherin immunodetection, the higher the age of the patients, a negative relationship between E-cadherin and MMP-2, and a positive association between MMP-9 and E-cadherin. Conclusions: The MMP-2, MMP-9, and E-cadherin are expressed in the endometrium of infertile patients during the receptive phase of the natural menstrual cycle. However, there is no correlation between the expression of these molecules and the clinical IVF outcomes.

show abstract

“…We and others previously reported that cancer cell invasiveness may be increased by IR in a surviving population after IR treatment. 16,17,39 Thus, we investigated the effect of RGD/ P-AuNPs on breast cancer invasion after radiation treatment. To evaluate the effect of RGD/P-AuNPs and radiation on the invasive activity of breast cancer cells, we performed a Matrigel chemoinvasion assay.…”

Section: Inhibition Of Radiation-induced Invasion By Rgd/p-aunps In Bmentioning

confidence: 99%

Targeting integrins with RGD-conjugated gold nanoparticles in radiotherapy decreases the invasive activity of breast cancer cells

Wu¹,

Onodera²,

Ichikawa³

et al. 2017

IJN

Self Cite

108

View full text Add to dashboard Cite

Gold nanoparticles (AuNPs) have recently attracted attention as clinical agents for enhancing the effect of radiotherapy in various cancers. Although radiotherapy is a standard treatment for cancers, invasive recurrence and metastasis are significant clinical problems. Several studies have suggested that radiation promotes the invasion of cancer cells by activating molecular mechanisms involving integrin and fibronectin (FN). In this study, polyethylene-glycolylated AuNPs (P-AuNPs) were conjugated with Arg–Gly–Asp (RGD) peptides (RGD/P-AuNPs) to target cancer cells expressing RGD-binding integrins such as α5- and αv-integrins. RGD/P-AuNPs were internalized more efficiently and colocalized with integrins in the late endosomes and lysosomes of MDA-MB-231 cells. A combination of RGD/P-AuNPs and radiation reduced cancer cell viability and increased DNA damage compared to radiation alone in MDA-MB-231 cells. Moreover, the invasive activity of breast cancer cell lines after radiation treatment was significantly inhibited in the presence of RGD/P-AuNPs. Microarray analyses revealed that the expression of FN in irradiated cells was suppressed by combined use of RGD/P-AuNPs. Reduction of FN and downstream signaling may be involved in suppressing radiation-induced invasive activity by RGD/P-AuNPs. Our study suggests that RGD/P-AuNPs can target integrin-overexpressing cancer cells to improve radiation therapy by suppressing invasive activity in addition to sensitization. Thus, these findings provide a possible clinical strategy for using AuNPs to treat invasive breast cancer following radiotherapy.

show abstract

β1-integrin via NF-κB signaling is essential for acquisition of invasiveness in a model of radiation treated in situ breast cancer

Cited by 34 publications

References 38 publications

Molecular Signatures of Membrane Protein Complexes Underlying Muscular Dystrophy

Molecular Signatures of Membrane Protein Complexes Underlying Muscular Dystrophy

Matrix Metalloproteinases 2 and 9 and E-Cadherin Expression in the Endometrium During the Implantation Window of Infertile Women Before In Vitro Fertilization Treatment

Targeting integrins with RGD-conjugated gold nanoparticles in radiotherapy decreases the invasive activity of breast cancer cells

Contact Info

Product

Resources

About