βA425–35 modulates substance P effect on rat skin microvasculature in aged rats: pharmacological manipulation using SEC-receptor ligands

Khalil, Zeinab; Sanderson, Katarina; Isberg, Pernilla; Bassirat, Maryam; Livett, Bruce G.; Helme, Robert D.

doi:10.1016/0006-8993(94)90701-3

Cited by 11 publications

(9 citation statements)

References 23 publications

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“…2. Scatchard analysis of Hep G2 (high) binding revealed a Kd of 48.7 ± 2.4 nM, consistent with previous reports (13,16,18,27,28). HuH7 cells bound more C105Y [1.5-fold more than Hep G2 (high)] with a Kd of76.3 ± 7.3 nM.…”

Section: Determination Of Surface Receptor Bindingsupporting

confidence: 91%

“…It recognizes a sequence on the carboxy-terminal tail of α 1 -antitrypsin (α 1 -AT) (27). A synthetic peptide (peptide 105Y) based in sequence on amino acids 359-37 4 of α 1 -AT bound to Hep G2 cells in a manner both specific and saturable (13,16,18,27,28). Scatchard analysis estimated a dissociation constant (Kd) of ~40 nM and ~405,000 plasma membrane receptors per cell.…”

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confidence: 99%

“…Compatible sequences are also found in some tachykinins, including substance P and substance K, amyloid-13 peptide, and bombesin (14,15). Thus, similar to other receptors favorable for gene transfer, it is adapted for binding and internalizing large molecular complexes with relatively low selectivity as long as a pentapeptide binding domain FV(F/ Y)L(IIM) is present (13,15,16,18,26,27).…”

mentioning

confidence: 99%

“…Furthermore, its presence in the brain may provide the potential to transfer therapeutic genes across the blood-brain barrier. In this study we tested the hypothesis that foreign DNA condensed by poly-L-lysine coupled to a synthetic peptide ligand for the SECR (13,18) can be targeted to and expressed in cells bearing the receptor in vitro.…”

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confidence: 99%

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Gene transfer into hepatoma cell lines via the serpin enzyme complex receptor

Ziady

Perales

Ferkol

et al. 1997

American Journal of Physiology-Gastrointestinal and Liver Physi

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The serpin enzyme complex receptor (SECR) expressed on hepatocytes binds to a conserved sequence in α 1 -antitrypsin (α 1 -AT) and other serpins. A molecular conjugate consisting of a synthetic peptide (C1315) based on the SECR binding motif of human α 1 -AT covalently coupled to poly-L-lysine was used to introduce reporter genes into hepatoma cell lines in culture. This conjugate condensed DNA into spheroidal particles 18-25 nm in diameter. When transfected with the SECR-directed complex containing pGL3, Hep G2 cells that express the receptor, but not Rep G2 cells that do not, expressed a peak luciferase activity of 538,731 ± 144,346 integrated light units/mg protein 4 days after transfection. Free peptide inhibited uptake and expression in a dosedependent manner. Complexes of DNA condensed with polyly-sine or LC-sulfo-Nsuccinimidyl-3-(2-pyridyldithio )propionate-substituted polylysine were ineffective. Transfection with a plasmid encoding human factor IX produced expression in Hep G2 (high) and HuH7 cells that express SECR but not Hep G2 (low) cells that lack the receptor. Fluorescein-labeled C1315 peptide labeled 9-31% of Hep G2 (high), 10-14% of HuH7, and 0.6-3.4% ofHep G2 (low) cells, and when the lac Z gene was transfected, only these cells expressed β-galactosidase. SECRmediated gene transfer gives efficient, specific uptake and high-level expression of three reporter genes, and the system merits further study for gene therapy.

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Section: Determination Of Surface Receptor Bindingsupporting

confidence: 91%

mentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Gene transfer into hepatoma cell lines via the serpin enzyme complex receptor

Ziady

Perales

Ferkol

et al. 1997

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Muscle sympathetic nerve activity is reduced by the leg resistance vasculature of the conduit arteries in older humans (Parker et al, 2007). In general, decreases in neuropeptide synthesis, content and release contribute to the decrease in neurogenic inflammation in old rats (Khalil et al, 1994) and also to the decrease in the vascular response to electrical stimulation of sensory nerves. Physiological responses involving sensory nerves decrease due to aging (Helme et al, 1985, Ardron et al, 1990).…”

Section: Introductionmentioning

confidence: 99%