βPS-Integrin acts downstream of Innexin 2 in modulating stretched cell morphogenesis in the <i>Drosophila</i> ovary

Huang, Yi Chia; Chen, Kuan Han; Chen, Yu Yang; Tsao, Liang Hsuan; Yeh, Tsung Han; Chen, Yu‐Chia; Wu, Ping Yen; Wang, Tsu Wei; Yu, Jenn Yah

doi:10.1093/g3journal/jkab215

Cited by 3 publications

(3 citation statements)

References 69 publications

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“…In addition to their roles in GJ or hemi-channel transport, ogre and Inx2 act as adaptor proteins independent of channel function to regulate intercellular interactions in several contexts. For example, Inx2 acts upstream of integrin-based adhesion to promote follicle cell flattening during Drosophila ovary morphogenesis [ 75 ], and both Inx2 and ogre play channel-independent adhesive roles in coupling subperineurial and wrapping glia [ 51 ]. Within the embryonic epidermis, Inx2 interacts with SJ and AJ proteins to control localization of junctional proteins, including E-cadherin, and epithelial polarization [ 52 , 76 ], but postembryonic epidermal Inx functions have not been defined.…”

Section: Discussionmentioning

confidence: 99%

Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli in Drosophila larvae

Luedke,

Yoshino,

Yin

et al. 2024

PLoS Genet

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An animal’s skin provides a first point of contact with the sensory environment, including noxious cues that elicit protective behavioral responses. Nociceptive somatosensory neurons densely innervate and intimately interact with epidermal cells to receive these cues, however the mechanisms by which epidermal interactions shape processing of noxious inputs is still poorly understood. Here, we identify a role for dendrite intercalation between epidermal cells in tuning sensitivity of Drosophila larvae to noxious mechanical stimuli. In wild-type larvae, dendrites of nociceptive class IV da neurons intercalate between epidermal cells at apodemes, which function as body wall muscle attachment sites, but not at other sites in the epidermis. From a genetic screen we identified miR-14 as a regulator of dendrite positioning in the epidermis: miR-14 is expressed broadly in the epidermis but not in apodemes, and miR-14 inactivation leads to excessive apical dendrite intercalation between epidermal cells. We found that miR-14 regulates expression and distribution of the epidermal Innexins ogre and Inx2 and that these epidermal gap junction proteins restrict epidermal dendrite intercalation. Finally, we found that altering the extent of epidermal dendrite intercalation had corresponding effects on nociception: increasing epidermal intercalation sensitized larvae to noxious mechanical inputs and increased mechanically evoked calcium responses in nociceptive neurons, whereas reducing epidermal dendrite intercalation had the opposite effects. Altogether, these studies identify epidermal dendrite intercalation as a mechanism for mechanical coupling of nociceptive neurons to the epidermis, with nociceptive sensitivity tuned by the extent of intercalation.

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Section: Discussionmentioning

confidence: 99%

Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli in Drosophila larvae

Luedke,

Yoshino,

Yin

et al. 2024

PLoS Genet

View full text Add to dashboard Cite

show abstract

“…In addition to their roles in GJ or hemi-channel transport, ogre and Inx2 act as adaptor proteins independent of channel function to regulate intercellular interactions in several contexts. For example, Inx2 acts upstream of integrin-based adhesion to promote follicle cell flattening during Drosophila ovary morphogenesis (69), and both Inx2 and ogre play channel-independent adhesive roles in coupling subperineurial and wrapping glia (47). Within the embryonic epidermis, Inx2 interacts with SJ and AJ proteins to control localization of junctional proteins, including E-cadherin, and epithelial polarization (49,70), but postembryonic epidermal Inx functions have not been defined.…”

Section: Discussionmentioning

confidence: 99%

Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli inDrosophilalarvae

Luedke,

Yoshino,

Yin

et al. 2023

Preprint

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An animal's skin provides a first point of contact with the sensory environment, including noxious cues that elicit protective behavioral responses. Nociceptive somatosensory neurons densely innervate and intimately interact with epidermal cells to receive these cues, however the mechanisms by which epidermal interactions shape processing of noxious inputs is still poorly understood. Here, we identify a role for dendrite intercalation between epidermal cells in tuning sensitivity of Drosophila larvae to noxious mechanical stimuli. In wild-type larvae, dendrites of nociceptive class IV da neurons intercalate between epidermal cells at apodemes, which function as body wall muscle attachment sites, but not at other sites in the epidermis. From a genetic screen we identified miR-14 as a regulator of dendrite positioning in the epidermis: miR-14 is expressed broadly in the epidermis but not in apodemes, and miR-14 inactivation leads to excessive apical dendrite intercalation between epidermal cells. We found that miR-14 regulates expression and distribution of the epidermal Innexins ogre and Inx2 and that these epidermal gap junction proteins restrict epidermal dendrite intercalation. Finally, we found that altering the extent of epidermal dendrite intercalation had corresponding effects on nociception: increasing epidermal intercalation sensitized larvae to noxious mechanical inputs and increased mechanically evoked calcium responses in nociceptive neurons, whereas reducing epidermal dendrite intercalation had the opposite effects. Altogether, these studies identify epidermal dendrite intercalation as a mechanism for mechanical coupling of nociceptive neurons to the epidermis, with nociceptive sensitivity tuned by the extent of intercalation.

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“…Innexin, a transmembrane protein, is a unique protein that constitutes the gap junction semi-channel in invertebrates and plays a key role in embryonic development, organogenesis, cellular immune response and cell apoptosis [59][60][61][62][63]. Some studies have shown that Innexin also plays a key role in the regulation of microtubule assembly [64,65]. In our previous study, the Innexin mRNA was specifically localized in circumoral ciliary cells of U. unicinctus larvae [27].…”

Section: Possible Key Genes and Pathways For Ciliogenesis On The Body...mentioning

confidence: 97%

Morphological Observation and Transcriptome Analysis of Ciliogenesis in Urechis unicinctus (Annelida, Echiura)

Kong

Wei

Liu

et al. 2023

IJMS

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During the early development of marine invertebrates, planktic larvae usually occur, and their body surfaces often form specific types of cilia that are involved in locomotion and feeding. The echiuran worm Urechis unicinctus sequentially undergoes the formation and disappearance of different types of body surface cilia during embryonic and larval development. The morphological characteristics and molecular mechanisms involved in the process remain unclear. In this study, we found that body surface cilia in U. unicinctus embryos and larvae can be distinguished into four types: body surface short cilia, apical tufts, circumoral cilia and telotrochs. Further, distribution and genesis of the body surface cilia were characterized using light microscope and electron microscope. To better understand the molecular mechanism during ciliogenesis, we revealed the embryonic and larval transcriptome profile of the key stages of ciliogenesis in U. unicinctus using RNA-Seq technology. A total of 29,158 differentially expressed genes (DEGs) were obtained from 24 cDNA libraries by RNA-Seq. KEGG pathway enrichment results showed that Notch, Wnt and Ca2+ signaling pathways were significantly enriched during the occurrence of apical tufts and circumoral cilia. Furthermore, all DEGs were classified according to their expression pattern, and DEGs with similar expression pattern were grouped into a module. All DEG co-expression modules were correlated with traits (body surface short cilia, apical tufts, circumoral cilia and telotrochs) by WGCNA, the results showed DEGs were divided into 13 modules by gene expression patterns and that the genes in No. 7, No. 8 and No. 10 modules were to be highly correlated with the occurrence of apical tufts, circumoral cilia and telotrochs. The top 10 hub genes in the above three modules were identified to be highly correlated with ciliogenesis, including the reported cilium-related gene Cnbd2 and unreported cilium-related candidate genes FAM181B, Capsl, Chst3, TMIE and Innexin. Notably, Innexin was included in the top10 hub genes of the two modules (No. 7 and No. 8), suggesting that Innexin may play an important role in U. unicinctus apical tufts, circumoral cilia and telotrochs genesis. This study revealed the characteristics of ciliogenesis on the body surface of U. unicinctus embryos and larvae, providing basic data for exploring the molecular mechanism of ciliogenesis on the body surface.

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βPS-Integrin acts downstream of Innexin 2 in modulating stretched cell morphogenesis in the Drosophila ovary

Cited by 3 publications

References 69 publications

Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli in Drosophila larvae

Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli in Drosophila larvae

Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli inDrosophilalarvae

Morphological Observation and Transcriptome Analysis of Ciliogenesis in Urechis unicinctus (Annelida, Echiura)

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