ε-Caprolactam Utilization by Proteus sp. and Bordetella sp. Isolated From Solid Waste Dumpsites in Lagos State, Nigeria, First Report

Sanuth, Hassan Adeyemi; Yadav, Amit; Fagade, O. E.; Shouche, Yogesh S.

doi:10.1007/s12088-013-0356-5

Cited by 9 publications

(6 citation statements)

References 15 publications

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“…Sanuth et al . [128] identified a P. mirabilis strain which was the most effective in ɛ-caprolactam degradation among the bacteria isolated from soils collected from the major solid waste dumpsites in Lagos State, Nigeria. ɛ-Caprolactam is a monomer for nylon-6 production, found in wastewater effluents from nylon-producing factories, toxic to plants, animals, and humans.…”

Section: Proteus In Bioremediation and Plant Growth Promotionmentioning

confidence: 99%

Significance and Roles of Proteus spp. Bacteria in Natural Environments

2016

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Proteus spp. bacteria were first described in 1885 by Gustav Hauser, who had revealed their feature of intensive swarming growth. Currently, the genus is divided into Proteus mirabilis, Proteus vulgaris, Proteus penneri, Proteus hauseri, and three unnamed genomospecies 4, 5, and 6 and consists of 80 O-antigenic serogroups. The bacteria are known to be human opportunistic pathogens, isolated from urine, wounds, and other clinical sources. It is postulated that intestines are a reservoir of these proteolytic organisms. Many wild and domestic animals may be hosts of Proteus spp. bacteria, which are commonly known to play a role of parasites or commensals. However, interesting examples of their symbiotic relationships with higher organisms have also been described. Proteus spp. bacteria present in soil or water habitats are often regarded as indicators of fecal pollution, posing a threat of poisoning when the contaminated water or seafood is consumed. The health risk may also be connected with drug-resistant strains sourcing from intestines. Positive aspects of the bacteria presence in water and soil are connected with exceptional features displayed by autochthonic Proteus spp. strains detected in these environments. These rods acquire various metabolic abilities allowing their adaptation to different environmental conditions, such as high concentrations of heavy metals or toxic substances, which may be exploited as sources of energy and nutrition by the bacteria. The Proteus spp. abilities to tolerate or utilize polluting compounds as well as promote plant growth provide a possibility of employing these microorganisms in bioremediation and environmental protection.

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Section: Proteus In Bioremediation and Plant Growth Promotionmentioning

confidence: 99%

Significance and Roles of Proteus spp. Bacteria in Natural Environments

2016

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“…According to literature, most of the caprolactam-degrading bacteria reported are Gram negative for example Pseudomonas spp., Proteus sp., A. faecalis and others (Boronin et al 1986; Otzen et al 2018; Sanuth et al 2013; Baxi and Shah 2002) and few Gram positive caprolactam-degrading bacteria have been reported as compared to Gram negative bacteria. As compared to Gram negative bacteria, the more predominant members found in soil are the Gram positive bacteria (Maier and Pepper 2000) and such bacteria are also more inherently resistant to stress in environment (Schimel et al 2007; Mongodin et al 2006).…”

Section: Discussionmentioning

confidence: 96%

“…During aerobic microbial degradation, xenobiotic compounds maybe converted to non toxic products but this is at the cost of depletion of oxygen from the polluted environment and this is a pollutional load on the environment. However microorganisms of only a few genera are reported to utilize and degrade caprolactam (Shama and Wase 1981; Wang and Lee 2007; Sanuth et al 2013) and thus it may persist in environment for a varying and indefinite time period.…”

Section: Introductionmentioning

confidence: 99%

An Arthrobacter citreus strain suitable for degrading ε-caprolactam in polyamide waste and accumulation of glutamic acid

2019

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ε-Caprolactam-a toxic xenobiotic compound present in industrial polyamide waste was found to be degraded by caprolactam-degrading bacteria. Arthrobacter citreus was able to utilize up to 20 g ε-caprolactam/l as the sole source of carbon more efficiently as compared to the other Gram positive caprolactam-degrading bacteria Rhodococcus rhodochrous and Bacillus sphaericus. The cells of A. citreus remained viable in medium up to 40 g caprolactam/l. The degradation of 10 g caprolactam/l by A. citreus, when supplied as the sole source of carbon and nitrogen lead to the formation of 6-aminocaproic acid which was detected in broth and there was also an increase in the ammonium content. One of the other metabolites found to consistently accumulate in extracellular medium during the utilization of caprolactam by A. citreus was glutamic acid, though not reported in case of other caprolactam-degrading bacteria. A. citreus could metabolise caprolactam to form non toxic products such as 6-aminocaproic acid and glutamic acid which are amino acids of physiological and commercial importance. In the presence of 6-aminocaproic acid, the rate of caprolactam utilization by A. citreus was decreased but not inhibited and the viable count of cells was found to increase using both the substrates simultaneously. A. citreus was also suitable for degradation of caprolactam in presence of low phosphate as prevalent in soil, and in sterile soil without the supplementation of any other carbon or nitrogen, as well as in native non sterile soil where other microorganisms are present.

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“…Later works described caprolactam-utilizing bacteria of the genera Alcaligenes , Corynebacterium , Acinetobacter , Achromobacter , Arthrobacter , Microbacterium , Bacillus, and others [ 6 , 8 , 9 , 10 , 29 ]. A phylogenetic study of CAP degraders isolated from nylon-6 production wastes attributed them to three phyla: Proteobacteria (Beta- and Gammaproteobacteria), Actinobacteria, and Firmicutes [ 7 ]. The plasmid or chromosomal localization of CAP catabolism genes in degraders representing these taxonomic groups remains an open issue.…”

Section: Discussionmentioning

confidence: 99%

“…To date, CAP-degrading bacteria from various taxonomic groups have been described: proteobacteria, actinobacteria, and spore-forming bacteria [ 7 , 8 , 9 ]. Some of them utilize the xenobiotic as a sole source of carbon and nitrogen; others require additional sources of carbon or nitrogen.…”

Section: Introductionmentioning

confidence: 99%

Epsilon-Caprolactam- and Nylon Oligomer-Degrading Bacterium Brevibacterium epidermidis BS3: Characterization and Potential Use in Bioremediation

2023

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epsilon-Caprolactam (Caprolactam, CAP), a monomer of the synthetic non-degradable polymer nylon-6, is the major wastewater component in the production of caprolactam and nylon-6. Biological treatment of CAP, using microbes could be a potent alternative to the current waste utilization techniques. This work focuses on the characterization and potential use of caprolactam-degrading bacterial strain BS3 isolated from soils polluted by CAP production wastes. The strain was identified as Brevibacterium epidermidis based on the studies of its morphological, physiological, and biochemical properties and 16S rRNA gene sequence analysis. This study is the first to report the ability of Brevibacterium to utilize CAP. Strain BS3 is an alcalo- and halotolerant organism, that grows within a broad range of CAP concentrations, from 0.5 up to 22.0 g/L, optimally at 1.0–2.0 g/L. A caprolactam biodegradation experiment using gas chromatography showed BS3 to degrade 1.0 g/L CAP over 160 h. In contrast to earlier characterized narrow-specific CAP-degrading bacteria, strain BS3 is also capable of utilizing linear nylon oligomers (oligomers of 6-aminohexanoic acid), CAP polymerization by-products, as sole sources of carbon and energy. The broad range of utilized toxic pollutants, the tolerance for high CAP concentrations, as well as the physiological properties of B. epidermidis BS3, determine the prospects of its use for the biological cleanup of CAP and nylon-6 production wastes that contain CAP, 6-aminohexanoic acid, and low molecular weight oligomer fractions.

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ε-Caprolactam Utilization by Proteus sp. and Bordetella sp. Isolated From Solid Waste Dumpsites in Lagos State, Nigeria, First Report

Cited by 9 publications

References 15 publications

Significance and Roles of Proteus spp. Bacteria in Natural Environments

Significance and Roles of Proteus spp. Bacteria in Natural Environments

An Arthrobacter citreus strain suitable for degrading ε-caprolactam in polyamide waste and accumulation of glutamic acid

Epsilon-Caprolactam- and Nylon Oligomer-Degrading Bacterium Brevibacterium epidermidis BS3: Characterization and Potential Use in Bioremediation

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