Μicropropagation and cultivation of Salvia sclarea for essential oil and sclareol production in northern Greece

Grigoriadou, Katerina; Trikka, Fotini A.; Tsoktouridis, Georgios; Krigas, Nikos; Sarropoulou, Virginia; Papanastasi, Katerina; Maloupa, Eleni; Makris, Antonios M.

doi:10.1007/s11627-019-10040-4

Cited by 23 publications

(30 citation statements)

References 25 publications

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“…The cultivation of aromatic plants, such as clary sage (Salvia sclarea L.), belonging to family Lamiaceae, could nonetheless represent a profitable and innovative alternative for the reconversion of agricultural contaminated soils. In particular, due to its biennial or shortlived perennial life-cycle, clary sage is able to quickly develop a sustainable vegetative cover over a 2-or 3-year period, and to produce large amounts of biomass (Tibaldi et al, 2010;Lydakis-Simantiris et al, 2016;Grigoriadou et al, 2020). Moreover, the cultivation of aromatic crops such as clary sage, for phytostabilization purposes on TE-contaminated sites, has previously been brought forward as a possible alternative, owing to the good quality of the produced EO regarding a potential TE-contamination (Zheljazkov et al, 2008;Angelova et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The Aromatic Plant Clary Sage Shaped Bacterial Communities in the Roots and in the Trace Element-Contaminated Soil More Than Mycorrhizal Inoculation – A Two-Year Monitoring Field Trial

et al. 2020

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To cope with soil contamination by trace elements (TE), phytomanagement has attracted much attention as being an eco-friendly and cost-effective green approach. In this context, aromatic plants could represent a good option not only to immobilize TE, but also to use their biomass to extract essential oils, resulting in high added-value products suitable for non-food valorization. However, the influence of aromatic plants cultivation on the bacterial community structure and functioning in the rhizosphere microbiota remains unknown. Thus, the present study aims at determining in TE-aged contaminated soil (Pb – 394 ppm, Zn – 443 ppm, and Cd – 7ppm, respectively, 11, 6, and 17 times higher than the ordinary amounts in regional agricultural soils) the effects of perennial clary sage (Salvia sclarea L.) cultivation, during two successive years of growth and inoculated with arbuscular mycorrhizal fungi, on rhizosphere bacterial diversity and community structure. Illumina MiSeq amplicon sequencing targeting bacterial 16S rRNA gene was used to assess bacterial diversity and community structure changes. Bioinformatic analysis of sequencing datasets resulted in 4691 and 2728 bacterial Amplicon Sequence Variants (ASVs) in soil and root biotopes, respectively. Our findings have shown that the cultivation of clary sage displayed a significant year-to-year effect, on both bacterial richness and community structures. We found that the abundance of plant-growth promoting rhizobacteria significantly increased in roots during the second growing season. However, we didn’t observe any significant effect of mycorrhizal inoculation neither on bacterial diversity nor on community structure. Our study brings new evidence in TE-contaminated areas of the effect of a vegetation cover with clary sage cultivation on the microbial soil functioning.

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Section: Introductionmentioning

confidence: 99%

The Aromatic Plant Clary Sage Shaped Bacterial Communities in the Roots and in the Trace Element-Contaminated Soil More Than Mycorrhizal Inoculation – A Two-Year Monitoring Field Trial

et al. 2020

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“…Essential oils, as high-added value products, may be valuable in various fields, such as perfumery and cosmetics, medicine, or food industries. As an example, clary sage (Salvia sclarea L.) is a xerophytic aromatic plant native to the Mediterranean area, which is able to grow relatively quickly and to develop a sustainable vegetative cover over two or three successive years, thanks to its short-lived perennial life cycle [24][25][26][27]. This Lamiaceae is also able to produce large amounts of biomass, especially in its second cultivation year, resulting in significant EO production, which is valuable to the food and cosmetics industries but is also well known for its antimicrobial and antiseptic properties [24,[27][28][29].…”

Section: Introductionmentioning

confidence: 99%

“…25 55.5 N, 3 • 02 25.5 E; altitude 23 m) in the north of France. The area is characterized by a warm, temperate, and humid climate with cool summers.…”

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Clary Sage Cultivation and Mycorrhizal Inoculation Influence the Rhizosphere Fungal Community of an Aged Trace-Element Polluted Soil

et al. 2021

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Soil fungal communities play a central role in natural systems and agroecosystems. As such, they have attracted significant research interest. However, the fungal microbiota of aromatic plants, such as clary sage (Salvia sclarea L.), remain unexplored. This is especially the case in trace element (TE)-polluted conditions and within the framework of phytomanagement approaches. The presence of high concentrations of TEs in soils can negatively affect not only microbial diversity and community composition but also plant establishment and growth. Hence, the objective of this study is to investigate the soil fungal and arbuscular mycorrhizal fungi (AMF) community composition and their changes over time in TE-polluted soils in the vicinity of a former lead smelter and under the cultivation of clary sage. We used Illumina MiSeq amplicon sequencing to evaluate the effects of in situ clary sage cultivation over two successive years, combined or not with exogenous AMF inoculation, on the rhizospheric soil and root fungal communities. We obtained 1239 and 569 fungal amplicon sequence variants (ASV), respectively, in the rhizospheric soil and roots of S. sclarea under TE-polluted conditions. Remarkably, 69 AMF species were detected at our experimental site, belonging to 12 AMF genera. Furthermore, the inoculation treatment significantly shaped the fungal communities in soil and increased the number of AMF ASVs in clary sage roots. In addition, clary sage cultivation over successive years could be one of the explanatory parameters for the inter-annual variation in both fungal and AMF communities in the soil and root biotopes. Our data provide new insights on fungal and AMF communities in the rhizospheric soil and roots of an aromatic plant, clary sage, grown in TE-polluted agricultural soil.

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“…Additionally, other biological activities, such as antiproliferative [5], anxiolytic [6,7], potentially anti-inflammatory [8] and sedative [9] effects, have been described. A number of representatives of this genus, like Salvia officinalis L. and S. sclarea L., are being cultivated worldwide for EO production [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…Salvia sclarea (clary sage) is typical for the European Mediterranean region and Africa to the Atlantic Ocean and widespread throughout the rest of the world [10]. It is a much-appreciated medicinal herb, also used in perfumery and cosmetics [11] as well as in pharmacy [3,12]. Most important components of clary sage essential oil (CSEO) are linalylacetate, linalool, germacrene D, αterpineol, and sclareol (▶ Fig.…”

Section: Introductionmentioning

confidence: 99%

Clary Sage Essential Oil and Its Effect on Human Mood and Pulse Rate: An in vivo Pilot Study

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et al. 2020

Planta Med

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Clary sage (Salvia sclarea) is a xerophytic biennial plant typical for the Mediterranean region. It is cultivated for its essential oil, which is widely used in the flavor and fragrance industry as well as in aromatherapy for its relaxing and immunomodulatory effect. In this pilot investigation, clary sage essential oil was analyzed by GC-MS as well as GC-FID and examined regarding its effects on pulse, blood pressure and mood in healthy human female and male subjects. Analysis of the used CSEO identified linalylacetat (61.3%) and linalool (22.1%) as main compounds. Sclareol was found at 0.3%. In the first experiment with 30 participants (with 15 female), CSEO, diluted in peanut oil, was administered to the left forearm, leading to an increase in pulse rate in women (peanut oil served as control). In men, pulse rate deceased over time (p = 0.013). In the second experiment (32 participants; 16 females) the oil was inhaled for 30 min (pure water as control). The observed decrease of pulse rate was significantly stronger in women than in men (p = 0.026). To evaluate the influence of the mode of application, a comparison of both experiments was performed showing a significant difference between sexes for the parameter of pulse rate (p = 0.034). Overall, the effects elicited by CSEO may depend on application method (inhaled/dermal) and sex (male/female).

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Μicropropagation and cultivation of Salvia sclarea for essential oil and sclareol production in northern Greece

Cited by 23 publications

References 25 publications

The Aromatic Plant Clary Sage Shaped Bacterial Communities in the Roots and in the Trace Element-Contaminated Soil More Than Mycorrhizal Inoculation – A Two-Year Monitoring Field Trial

The Aromatic Plant Clary Sage Shaped Bacterial Communities in the Roots and in the Trace Element-Contaminated Soil More Than Mycorrhizal Inoculation – A Two-Year Monitoring Field Trial

Clary Sage Cultivation and Mycorrhizal Inoculation Influence the Rhizosphere Fungal Community of an Aged Trace-Element Polluted Soil

Clary Sage Essential Oil and Its Effect on Human Mood and Pulse Rate: An in vivo Pilot Study

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