σ ^P -NagA-L1/L2 Regulatory Circuit Involved in ΔompA _299-356 -Mediated Increase in β-Lactam Susceptibility in Stenotrophomonas maltophilia

Abstract

Porins of Gram-negative bacteria generally act as channels that allow the entry or extrusion of molecules. Moreover, the structural role of porins in stabilizing the outer membrane by interacting with peptidoglycan (PG) and the outer membrane has been proposed.

Implication of the σ E Regulon Members OmpO and σ N in the Δ ompA 299–356 -Mediated Decrease of Oxidative Stress Tolerance in Stenotrophomonas maltophilia

“…Recently, we reported that ompA is highly expressed in logarithmic-phase S. maltophilia KJ cells ( 38 ). We characterized an in-frame deletion ompA mutant of S. maltophilia KJ, originally termed KJΔOmpA ( 39 ) and later renamed KJΔOmpA 299–356 ( 40 ). We also reported that the truncated OmpA protein can be stably embedded in the outer membrane but loses contact with peptidoglycan (PG) ( 40 ).…”

“…We characterized an in-frame deletion ompA mutant of S. maltophilia KJ, originally termed KJΔOmpA ( 39 ) and later renamed KJΔOmpA 299–356 ( 40 ). We also reported that the truncated OmpA protein can be stably embedded in the outer membrane but loses contact with peptidoglycan (PG) ( 40 ). KJΔOmpA 299–356 exhibits decreased conjugation ability and swimming motility ( 39 ), as well as increased susceptibility to β-lactams ( 40 ).…”

“…We also reported that the truncated OmpA protein can be stably embedded in the outer membrane but loses contact with peptidoglycan (PG) ( 40 ). KJΔOmpA 299–356 exhibits decreased conjugation ability and swimming motility ( 39 ), as well as increased susceptibility to β-lactams ( 40 ). Transcriptome analysis revealed that the expression levels of the three σ factors, rpoN , rpoP , and rpoE , are significantly altered in KJΔOmpA 299–356 ( 39 , 40 ).…”

“…KJΔOmpA 299–356 exhibits decreased conjugation ability and swimming motility ( 39 ), as well as increased susceptibility to β-lactams ( 40 ). Transcriptome analysis revealed that the expression levels of the three σ factors, rpoN , rpoP , and rpoE , are significantly altered in KJΔOmpA 299–356 ( 39 , 40 ). Notably, rpoN downregulation is the key factor contributing to the swimming defect in KJΔOmpA 299–356 , and upregulated σ P is involved in the Δ ompA 299–356 -mediated increase in β-lactam susceptibility via the σ P -NagA-L1/L2 regulatory circuit ( 39 , 40 ).…”

“…Transcriptome analysis revealed that the expression levels of the three σ factors, rpoN , rpoP , and rpoE , are significantly altered in KJΔOmpA 299–356 ( 39 , 40 ). Notably, rpoN downregulation is the key factor contributing to the swimming defect in KJΔOmpA 299–356 , and upregulated σ P is involved in the Δ ompA 299–356 -mediated increase in β-lactam susceptibility via the σ P -NagA-L1/L2 regulatory circuit ( 39 , 40 ). Here, we aimed to further investigate the effect of Δ ompA 299–356 on oxidative stress tolerance and elucidate the underlying mechanism.…”