ВАЛИДАЦИЯ СВЯЗИ ГЕННОГО ПОЛИМОРФИЗМА С ПОВЫШЕННОЙ ЧАСТОТОЙ ХРОМОСОМНЫХ АБЕРРАЦИЙ У РАБОТНИКОВ РАДИАЦИОННОГО ПРОИЗВОДСТВА, "Радиационная Биология. Радиоэкология"

Халюзова, М. В.; Литвяков, Н. В.; Исубакова, Д. С.; Брониковская, Е. В.; Usova, T. V.; Альбах, Е.Н.; Карпов, А. Б.; Семёнова, А.А.; Takhauov, R. M.

doi:10.7868/s0869803117040038

Cited by 4 publications

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“…В настоящее время одним из востребованных и хорошо изученных методов для индикации дозы ИИ и оценки степени радиационного воздействия является цитогенетический анализ лимфоцитов крови [10,11].…”

Section: результаты и обсуждениеunclassified

Comparative Retrospective Analysis of the Results of Cytogenetic Studies of Employees of the Object of Use of Ionizing Radiation

Vishnevskaya,

Isubakova,

Tsyplenkova

et al. 2024

MEDICAL RADIOLOGY AND RADIATION SAFETY

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Purpose: To conduct a comparative retrospective analysis of the results of cytogenetic studies of workers at facilities using ionizing radiation in 2003 and 2018. Material and methods: The material for the study was the venous blood of workers at facilities using ionizing radiation. The study was conducted on conditionally healthy workers (n = 11), of which 2 groups were formed: the control group (blood sampling and cytogenetic study of blood lymphocytes were carried out in 2003) and the study group (blood sampling was carried out in 2018). For all examined individuals, blood lymphocytes were cultured and standard cytogenetic analysis followed by statistical processing of the results. Results: A comparative retrospective analysis showed that in the study group (2018) compared to the control group (2003), the frequency of chromatid fragments was reduced (p = 0.0452). The frequencies of other types of cytogenetic disorders studied (aberrant cells, chromosomal fragments, dicentric and circular chromosomes) do not differ between groups. Cytogenetic abnormalities in blood lymphocytes are a highly sensitive measure of the degree of radiation exposure in the early and late periods after exposure and can be used as a biological indicator of ionizing radiation. The absence of differences in indicators of radiation exposure markers may be due to the large interval of time that elapsed after irradiation until the moment of examination (15 years), during which lymphocytes with chromosomal aberrations were eliminated from the blood. In the future, for the completeness of the retrospective analysis and the accuracy of the result, it is planned to conduct a study on a larger sample with a shorter time interval between cytogenetic studies. Conclusion: The result of this work allows us to supplement the understanding of the mutation process in the somatic cells of individuals exposed to ionizing radiation in the course of professional activities and indicates genotoxicity.

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Section: результаты и обсуждениеunclassified

Comparative Retrospective Analysis of the Results of Cytogenetic Studies of Employees of the Object of Use of Ionizing Radiation

Vishnevskaya,

Isubakova,

Tsyplenkova

et al. 2024

MEDICAL RADIOLOGY AND RADIATION SAFETY

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The Bank of Biological Samples by Seversk Biophysical Research Center

Takhauova

Исубакова

Bronikovskaya³

et al. 2020

Medical Radiology and Radiation Safety

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Purpose: Description of the collection, structure and development dynamics during the period from 2015 to 2019 of the biological material bank of the Seversk Biophysical Research Center, as well as research works carried out using biological material from its collection. Material and methods: The collection of bank of biological material includes the following types of biological material: venous blood, genomic DNA of white blood cells, cytogenetic suspensions of blood lymphocytes, surgical, biopsy and autopsy material. Biological material was obtained from people exposed to chronic radiation exposure: employees of the nuclear industry and the population permanently residing in the territory located in the area of the nuclear industry. Results: At present, the collection of the biological material bank contains more than 21,000 samples of biological material obtained from more than 8,000 donors. The collection of bank of biological material is represented by 4 main blocks: biological material of conditionally healthy employees of company of the nuclear industry (Siberian Chemical Combine); biological material of conditionally healthy people permanently residing in the territory located in the area of the nuclear industry enterprise (Seversk); biological material of patients (workers of the Siberian Chemical Combine and residents of Seversk) with malignant neoplasms and biological material of patients (workers of the Siberian Chemical Combine and residents of Seversk) with acute myocardial infarction. In the collection of bank of biological material, the proportion of samples of biological material of relatively healthy workers of the Siberian Chemical Combine is 33 %, residents of Seversk — 40.8 %, patients with malignant neoplasms — 22.2 %, patients with acute myocardial infarction — 4 %. Conclusion: Biological material bank Seversk Biophysical Research Center is unique collection of biological material samples of healthy people and patients with socially significant diseases. A feature of the biological material of the biological material bank is that it is obtained from people who have been exposed to chronic low-intensity radiation exposure and can be used to evaluate its radiogenic effects.

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Apoptosis of Lymphocytes and Polymorphisms of Apoptosis Regulation Genes in Individuals Exposed to Chronic Radiation Exposure

Блинова

Kotikova

Yanishevskaya

et al. 2020

Medical Radiology and Radiation Safety

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Purpose: Study the apoptotic death of peripheral blood lymphocytes in long-term period in persons exposed to chronic radiation exposure, and analysis of association of the polymorphic regions rs4645878, rs2279115, rs28362491, rs664677, rs1042522, rs1801270, rs2279744 of the BAX, BCL2, NFkB, ATM, TP53, CDKN1A, MDM2 genes with apoptotic lymphocytes frequency in residents of the coastal villages of the Techa River. Material and methods: The study of apoptosis and genotyping was conducted in 390 persons exposed to chronic radiation exposure as a result of Mayak PA radioactive waste releases into the Techa–Iset–Tobol river system. The early stage of apoptosis was assessed on a flow cytometer by the presence of phosphatidylserine on the surface of the cell membranes using the Annexin V Apoptosis Detection Kit I and the late stage of apoptosis using the TUNEL method. Real-time PCR genotyping was performed of allelic variations of rs4645878, rs2279115, rs28362491, rs664677, rs1042522, rs1801270, rs2279744 of BAX, BCL2, NFkB, ATM, TP53, CDKN1A, MDM2 genes in a group of irradiated individuals. Results: The number of cells at the early stage of apoptosis is statistically significantly increased in individuals whose irradiation began during the period of intrauterine development and continued in the postnatal period compared to individuals exposed only in the postnatal period. At the same time, the number of lymphocytes at the stage of DNA fragmentation in the group irradiated in utero is lower than in the group irradiated postnatally and non-irradiated individuals. Also, a weak negative correlation between intrauterine doses of RBM irradiation and the doses of thymus and peripheral lymphoid organs with the number of cells in the late stage of apoptosis in individuals irradiated in utero. The influence of allelic variation rs4645878 of the BAX gene was established on the number of lymphocytes at the early stage of apoptosis in residents of coastal villages of the Techa River. A statistically significant decrease in the number of cells at an early stage of apoptosis is observed in C/C genotype carriers according to the allelic variation rs4645878 of the BAX gene compared with carriers of the T/T and T/C genotypes. Conclusion: Residents of coastal villages of the Techa River exposed to radiation during the period of prenatal development, there are differences in the frequency of apoptotic death of peripheral blood lymphocytes compared with non-irradiated persons and persons who were irradiated in the postnatal period. SNPs of apoptosis-regulating genes can modify the response of blood lymphocytes to radiation in a wide range of doses RBM.

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Cited by 4 publications

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Comparative Retrospective Analysis of the Results of Cytogenetic Studies of Employees of the Object of Use of Ionizing Radiation

Comparative Retrospective Analysis of the Results of Cytogenetic Studies of Employees of the Object of Use of Ionizing Radiation

The Bank of Biological Samples by Seversk Biophysical Research Center

Apoptosis of Lymphocytes and Polymorphisms of Apoptosis Regulation Genes in Individuals Exposed to Chronic Radiation Exposure

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