ИММУНОЛОГИЧЕСКИ ЗНАЧИМЫЕ ГЛИКОПРОТЕИНЫ p54 И CD2v ВИРУСА АФРИКАНСКОЙ ЧУМЫ СВИНЕЙ: БИОИНФОРМАТИЧЕСКИЙ АНАЛИЗ ГЕНЕТИЧЕСКИХ ВАРИАЦИЙ И ГЕТЕРОГЕННОСТИ

Mima, K. A.; БУРМАКИНА, Г.С.; Titov, Ilya; Malogolovkin, Alexander

doi:10.15389/agrobiology.2015.6.785rus

S-h. biol.

2015

DOI: 10.15389/agrobiology.2015.6.785rus

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ИММУНОЛОГИЧЕСКИ ЗНАЧИМЫЕ ГЛИКОПРОТЕИНЫ p54 И CD2v ВИРУСА АФРИКАНСКОЙ ЧУМЫ СВИНЕЙ: БИОИНФОРМАТИЧЕСКИЙ АНАЛИЗ ГЕНЕТИЧЕСКИХ ВАРИАЦИЙ И ГЕТЕРОГЕННОСТИ

K. A. Mima¹,

Г.С. БУРМАКИНА²,

Ilya Titov³

et al.

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“…According to the results of our studies (seefig. 1), the studied proteins of the ASFV are conservative, especially in comparison with other ASFV variable proteins (CD2V, P54)[29].…”

mentioning

confidence: 97%

Analysis of the African Swine Fever Virus Immunomodulatory Proteins

Nefedeva

Titov

Mima

et al. 2019

Mol. Genet. Microbiol. Virol.

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Molecular epidemiology of viral infections traditionally based on the analysis of changes in individual genes or genetic markers. The analysis of the African swine fever virus (ASFV) genes encoding immunomodulatory proteins is an important tool for studying the diversity and evolution of the virus. In this work, we carried out a structural and phylogenetic analysis of the ASF virus immunomodulatory proteins 5EL (A238l gene), I14L (Dp71l gene), K11L (I329l gene). The degree of nucleotide substitutions of the ASFV concatenated genes A238l, I329l and Dp71l revealed purifying (stabilizing) selection at the nucleotide sequences level. The variability characteristic of the selected group of ASFV genes is of great interest for the genetic differences search in immunomodulatory proteins. The sequencing results of the A238l, I329l and Dp71l genes and their phylogenetic analysis showed that these genes are 2 conservative among a large group of ASFV genes. The I329l gene is a genetic marker of common origin. The East African strains (Genotype X) of Dp71l gene have two forms: a long (184 amino acids) and a short (from 70 to 72 amino acids) and is formed by fusion of the 13L and 14L. All ASF virus Russian isolates isolated in 2016-2017 were identical to the reference strain ASFV/Georgia/wb/2007. Characterization of variability 5EL protein, I14L, K11L may be serve to identify target sites in the ASFV genome and to develop vaccines. The obtained data allow to evaluate the genetic diversity of the ASFV immunomodulatory proteins and the dynamics of their evolution, to predict the possible participation of the A238l, I329l and Dp71l genes in the virulence of various ASFV strains.

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“…According to the results of our studies (seefig. 1), the studied proteins of the ASFV are conservative, especially in comparison with other ASFV variable proteins (CD2V, P54)[29].…”

mentioning

confidence: 97%

Analysis of the African Swine Fever Virus Immunomodulatory Proteins

Nefedeva

Titov

Mima

et al. 2019

Mol. Genet. Microbiol. Virol.

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Analysis of the african swine fever virus immunomodulatory proteins

Nefedeva¹,

Titov²,

Mima³

et al. 2019

Mol. genet. mikrobiol. virusol.

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Молекулярная эпидемиология вирусных инфекций традиционно основывается на анализе изменений отдельных генов или генетических маркеров. Анализ генов вируса африканской чумы свиней (АЧС), кодирующих иммуномодулирующие белки является важным инструментом изучения разнообразия и эволюции вируса. В данной работе мы провели структурный и филогенетический анализ иммуномодулирующих белков 5el (ген A238L), i14l (ген DP71L), k11l (ген I329L) вируса АЧС. Степень нуклеотидных замен конкатенированных генов A238L, I329L и DP71L вируса АЧС выявила очищающий (стабилизирующий) отбор на уровне нуклеотидных последовательностей. Характеристика вариабельности отобранной нами группы генов вируса АЧС представляет большой интерес для поиска генетических различий в иммуномодулирующих белках. Результаты секвенирования генов A238L, I329L и DP71L и их филогенетический анализ показали, что эти гены являются консервативными среди большой группы генов вируса АЧС. Ген I329L является генетическим маркером общности происхождения. Ген DP71L у восточноафриканских штаммов X генотипа имеет две формы: длинную (184 аминокислоты) и краткую (от 70 до 72 аминокислот) и образуется путем слияния 13L и 14L. Все российские изоляты вируса АЧС, выделенные в 2016-2017 гг., по изученным генам идентичны референтному штамму ASFV/Georgia/wb/2007. Характеристика вариабельности белков 5el, i14l, k11l может послужить выявлению таргетных участков в геноме вируса АЧС и для разработки вакцин. Полученные данные позволяют оценить генетическое разнообразие иммуномодулирующих белков вируса АЧС и динамику их эволюции, предсказать возможное участие генов A238L, I329L и DP71L в вирулентности различных штаммов вируса АЧС. Ключевые слова: вирус африканской чумы свиней, секвенирование, филогенетический анализ, иммуномодулирующие белки, анализ синонимичных и несинонимичных замен.

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In silico prediction of B- and T-cell epitopes in the CD2v protein of african swine fever virus (African swine fever virus, Asfivirus, Asfarviridae)

Mima¹,

Katorkina²,

Katorkin³

et al. 2020

Problems of Virology

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Introduction. African swine fever virus (ASF) is a large DNA virus that is the only member of the Asfarviridae family. The spread of the ASF virus in the territory of the Russian Federation, Eastern Europe and China indicates the ineffectiveness of existing methods of combating the disease and reinforces the urgent need to create effective vaccines. One of the most significant antigens required for the formation of immune protection against ASF is a serotype-specific CD2v protein.The purpose of the study. This study presents the results of immuno-informatics on the identification of B- and T-cell epitopes for the CD2v protein of the ASF virus using in silico prediction methods.Material and methods. The primary sequence of the CD2v protein of the ASFV virus strain Georgia 2007/1 (ID-FR682468) was analyzed in silico by programs BCPred, NetCTLpan, VaxiJen, PVS and Epitope Conservancy Analysis.Results. Using the BCPred and VaxiJen programs, 4 major B-cell immunogenic epitopes were identified. Analysis of the secretory region of ASF virus CD2v protein in NetCTLpan revealed 5 T-cell epitopes from the 32nd to the 197th position of amino acids that cross-link from the 1st to the 13th allele of the MHC-I of pigDiscussion. This study presents the results in silico prediction to identify B- and T-cell epitopes of ASF virus CD2v protein. The soluble region of the CD2v protein can be included in the recombinant polyepitope vaccine against African swine fever.Conclusion. B- and T-cell epitopes in the secretory region of the CD2v protein (from 17 to 204 aa) of ASF virus were identified by in silico prediction. An analysis of the conservatism of the identified B- and T-cell epitopes allowed us to develop a map of the distribution of immune epitopes in the CD2v protein sequence.

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ИММУНОЛОГИЧЕСКИ ЗНАЧИМЫЕ ГЛИКОПРОТЕИНЫ p54 И CD2v ВИРУСА АФРИКАНСКОЙ ЧУМЫ СВИНЕЙ: БИОИНФОРМАТИЧЕСКИЙ АНАЛИЗ ГЕНЕТИЧЕСКИХ ВАРИАЦИЙ И ГЕТЕРОГЕННОСТИ

Cited by 3 publications

References 0 publications

Analysis of the African Swine Fever Virus Immunomodulatory Proteins

Analysis of the African Swine Fever Virus Immunomodulatory Proteins

Analysis of the african swine fever virus immunomodulatory proteins

In silico prediction of B- and T-cell epitopes in the CD2v protein of african swine fever virus (African swine fever virus, Asfivirus, Asfarviridae)

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