Исследование Цитотоксичности Антиглаукомных Лекарственных Средств На Первичной Культуре Эндотелиальных Клеток Роговицы Человека

Fisenko, N V; Subbot, A.M.; Фисенко, Владимир Петрович

doi:10.30906/0869-2092-2022-85-8-26-33

Cited by 5 publications

(4 citation statements)

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“…Таким образом, представленные данные позволяют выделить офтальмогипертензию, глаукому и операции, направленные на снижение ВГД, в качестве факторов, приводящих к развитию эндотелиальной дисфункции роговицы или трансплантата. Их негативная роль, проявляющаяся снижением плотности метаболически активных ЭК на фоне локального острого и хронического воспалительного процесса, была ранее неоднократно подтверждена различными авторами [13][14][15][16].…”

Section: Discussionunclassified

Clinicopathologic Analyses of Failed Endothelial Keratoplasty (the Clinical Cases)

Yusef,

Fisenko,

Demura

et al. 2023

Oftalʹmologiâ

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Purpose: To evaluate the clinicopathologic features of failed endothelial keratoplasty. Methods. In this study 11 patients (11 eyes) with recurrence of bullous keratopathy (BK) were included. Group 1 consisted of 4 patients who underwent repeat Descemet Stripping Automated Endothelial Keratoplasty (DSAEK), group 2 included 7 patients who underwent penetrating keratoplasty (PK) after failed Descemet Membrane Endothelial Keratoplasty (DMEK). Preoperative anterior segment optical coherence tomography (OCT), RTVue-100, Optovue, USA, was performed. Intraoperatively aqueous humour (AqH) samples were collected for multiplex cytokine analysis. During keratoplasty failed grafts/corneal buttons were obtained and then investigated histologically (hematoxylin/eosin staining, primary antibodies to pancytokeratin, vimentin, collagen III). Results. Recurrence of BK in all clinical cases manifests by the increase of inflammatory factors in AqH, corneal edema, neovascularization and remodeling to fibrosis. Glaucoma may induce DMEK/ DSAEK graft failure due to chronic local inflammation. In the clinical cases recurrence of BK was caused by peripheral and central graft detachment, rebubbling, graft upside-down orientation and donor corneal genetic disorders. Conclusions. Chronic local inflammation (including corneal morphological changes) in patients with BK recurrence is an indication for reoperation. The modification of keratoplasty — DMEK/DSAEK/PK — is determined according to slit-lamp and OCT images of the cornea. High-level risk of immune reaction (especially in cases of three and more times repeated keratolasty) is the reason for systemic corticosteroid and, sometimes, cytostatic therapy.

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Section: Discussionunclassified

Clinicopathologic Analyses of Failed Endothelial Keratoplasty (the Clinical Cases)

Yusef,

Fisenko,

Demura

et al. 2023

Oftalʹmologiâ

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“…Наибольшую значимость среди супрессорных молекул имеют цитокины (интерлейкины). Все цитокины, присутствующие во ВГЖ и хрусталике, делятся на классы по доминантному биологическому действию: провоспалительные (IL-1, IL-6, IL-12, TNF-α, IFNγ, MCP-1, MIP-1β), противовоспалительные (IL4, IL-7, IL-8, IL-9, IL-10), факторы, влияющие на рост и дифференцировку лимфоцитов (IL-2, IL-4, IL-5, IL-13, IL-17), а также факторы роста (G-CSF, GM-CSF) [12,[37][38][39][40].…”

Section: иммунологические аспекты теории помутнения хрусталикаunclassified

“…Нельзя исключить, что это связано с избирательной проницаемостью иридоцилиарной (гематоцилиарной) части гематоофтальмического барьера. Кроме того, изменение молекулярного состава и конституциональный дисбаланс во ВГЖ часто являются причиной развития патологических процессов в переднем отрезке глаза [12].…”

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The Role of Biochemical Markers in Cataractogenesis. Literature Review

Alkharki,

Matyuschenko

2024

Oftalʹmologiâ

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Lens transparency is determined by both cellular and subcellular levels of its organization. Abnormalities of its size, uniformity of shape and correct arrangement of the fibers lead to the scattering of light falling on the lens. At the same time, its normal transparent proteins turn into a cloudy, coagulated, insoluble form, and undergo denaturation. Therefore, the preservation of lens transparency is possible only with a certain chemical composition, achieved by a strict balance of all metabolism links. There are many different hypotheses about the disease etiology. It is known that intraocular fluid has a low content of proteins and an increased concentration of chloride-, lactate-, ascorbatanions in its composition, in contrast to blood plasma. It cannot be ruled out that this is due to the selective permeability of the blood–ocular barrier, which consists of non-pigmented epithelial cells of the ciliary body. In addition, the modification of molecular composition and constitutional imbalance in the intraocular fluid often causes of pathological processes development in the anterior segment of the eye. The blood-ocular barrier makes the eye an immune-privileged organ. However, many diseases, surgical interventions and eye injuries can lead to blood-ocular barrier damage. This causes to inflammatory effector cells and molecules inducing a cascade of reactions, which in turn results in irreversible fibrotic changes in the lens substance. In this regard, it becomes necessary to search for new reliable methods of determining the level of certain biochemical agents in intraocular structures, as well as establishing reference values for strategically important biomarkers of cataract development. This review presents modern views on biochemical markers imbalance in the anterior chamber aqueous humor and the lens, which contributes to its substance opacity.

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“…при исследовании концентрации цитокинов, циркулирующих во ВГЖ, также обнаружили высокие показатели IL-6 у пациентов с вторичной БК различной этиологии, в том числе при наличии ПОУГ как коморбидной офтальмопатологии [18]. Сходные изменения состава ВГЖ были описаны при ЭД Фукса и псевдофакичной БК [17,25]. В то же время различными исследователями, независимо друг от друга, было показано Таблица 2.…”

Section: методыunclassified

The Influence of Glaucoma as a Comorbidity on Cytokine Expression in Aqueous Humor of Patients with Fuchs Endothelial Corneal Dystrophy and Bullous Keratopathy

et al. 2023

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Purpose: to evaluate the effect of primary open-angle glaucoma (POAG) as a comorbidity on cytokine expression in aqueous humor (AqH) of patients with Fuchs endothelial corneal dystrophy (FECD) and bullous keratopathy (BK).Patients and methods. In this prospective consecutive case study 58 patients (58 eyes) were divided into 2 main groups. Group 1 (22 patients with FECD) included 11 patients with coexisting POAG II–IIIA (group 1a) and 11 patients without ocular comorbidity (group 1b). Group 2 (28 patients with BK) consisted of 19 patients with coexisting POAG II–IIIA (group 2a) and 9 patients without ocular comorbidity (group 2b). Group 3 (control) included 8 patients with cataract. The patients of groups 1 and 2 underwent endothelial keratoplasty. Intraoperatively obtained recipients’ Descemet’s membranes (DMs) were investigated histologically (hematoxylin/eosin staining). A total of 58 AqH samples were collected from consecutive patients who underwent endothelial keratoplasty (groups 1a, 1b, 2a, 2b) or cataract surgery (controls). The AqH levels of cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17, G-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1β and TNF-α) were compared among the groups.Results. Clinical diagnoses of FECD (group 1) and BK (group 2) were confirmed by the results of morphologic study. The levels of IL-8, MCP-1, IFN-γ were significantly higher in AqH samples from FECD and BK groups (regardless of the presence or absence of POAG) compared with the controls. IL-6 level was significantly elevated in FECD with coexisting POAG (group 1a) and BK (groups 2a and 2b) than in the controls. The influence of POAG on the local inflammation in FECD and BK is confirmed by the increased level of MIP-1β and the low concentration of GM-CSF compared with the controls. Among BK eyes (groups 2a and 2b), POAG as a comorbidity (group 2a) was associated with decreased level of IL-12. FECD with coexisting POAG was characterized with lower level of IL-13 in AqH than in the controls.Conclusions. POAG as a comorbid pathology in patients with FECD leads to high immune response mediated by cytokines expression. BK regardless of coexisting POAG is associated with severe local immune inflammation.

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Исследование Цитотоксичности Антиглаукомных Лекарственных Средств На Первичной Культуре Эндотелиальных Клеток Роговицы Человека

Cited by 5 publications

References 0 publications

Clinicopathologic Analyses of Failed Endothelial Keratoplasty (the Clinical Cases)

Clinicopathologic Analyses of Failed Endothelial Keratoplasty (the Clinical Cases)

The Role of Biochemical Markers in Cataractogenesis. Literature Review

The Influence of Glaucoma as a Comorbidity on Cytokine Expression in Aqueous Humor of Patients with Fuchs Endothelial Corneal Dystrophy and Bullous Keratopathy

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