Нepatoprotective properties of polyphenolic complexes from woodand cellular culture of maackia amurensis

Abstract

Annot For finding-out of a role of separate groups of polyphenols in realization hepatoprotective properties of maksar we investigate activity of polyphenolic complexes (PPC) from duramen and cellular culture of maakia amur. Experiments are carried out on 40 not purebred white rats with an experimental СCl4-hepatites. Therapeutic efficiency of researched objects estimated on their influence on survival rate of animals, morphological and biochemical parameters of a liver and wheys of blood. In rat's CCl4-hepatitis PPC from duramen and cellular culture of maakia amur reduce the acute toxity of tetrachloromethane, decrease the necrosis of hepatocytes and cellural infiltration of liver's parenchyma, prevent from development of fatty and protein dystrophy of a liver, normalize the activity of amino transferase, ƒ-glutamyltransferase in blood, the concentration of proteins and lipids, stimulate conjugation of bilirubin. The therapeutic effect of PPC of cellular culture is more evident. The hepatoprotective action is caused by a presence of isoflavonoids daidzein, retuzin, genistein, afromozin, formononetin, orobol, tektorigenin, maakiain and medicarpin. PPC from a culture of cells doesn't influence on a level of cholesterol in blood, that apparently is caused by an absence of mono- and dimeric stilbenes in its structure.

Isoflavonoid Composition of a Callus Culture of the Relict Tree Maackia amurensis Rupr. et Maxim

“…Repeated column chromatography using Toyopearl HW-50F and Toyopearl HW-40F columns and preparative HPLC chromatography of the cell culture extract followed by recrystallization yielded 20 isoflavonoids, 6 of which were identified as free isoflavones ( 9 1 and 2). The physical, spectroscopic, and 1 H and 13 C NMR data for the isolated compounds showed identity with the following previously identified isoflavones: daidzein (9), calycosin (10), genistein (12), formononetin (13), pseudobaptigenin (14), and derrone (15); their β-glucosides and malonylglucosides, 4′-O-β-D-glucopyranosyldaidzin (1), 4′-O-β-D-glucopyranosylgenistin (2), daidzin (3), 3′-methoxydaidzin (4), 7-O-β-D-glucopyranosylcalycosin ( 5), genistin ( 6), 6′′-O-malonylgenistin (7), ononin (8), 6′′-O-malonylononin (11); and pterocarpans and their malonyl glucosides, (6aR,11aR)maackiain ( 19), (6aR,11aR)-medicarpin ( 20), (6aR,11aR)-6′-Omalonyl-3-O-β-D-glucopyranosylmaackiain ( 17), (6aR,11aR)-6′-O-malonyl-3-O-β-D-glucopyranosylmedicarpin (18). Only a few authors have provided full NMR spectral assignments for the glucosidic component of the conjugated forms of isoflavonoids.…”

“…Polyphenolic compounds extracted from both core wood and callus cultures of M. amurensis decreased the acute toxicity of tetratrachloromethane on liver with similar efficacy, showing a prominent favorable effect on animal survival (14). This effect resulted from the suppression of dystrophy and hepatocyte necrosis, normalization of aminotransferase and γ-glutamyltransferase activities in the blood, and stimulation of bilirubin conjugation (14). Thus, the possibility of creating a renewable source of Maackia isoflavonoids has been demonstrated.…”

“…Analyses have shown the presence of the isoflavones daidzein, retuzin, genistein, and formononetin and the pterocarpans maakiain and medicarpin, although these cultures did not contain stilbenes . Polyphenolic compounds extracted from both core wood and callus cultures of M. amurensis decreased the acute toxicity of tetratrachloromethane on liver with similar efficacy, showing a prominent favorable effect on animal survival . This effect resulted from the suppression of dystrophy and hepatocyte necrosis, normalization of aminotransferase and γ-glutamyltransferase activities in the blood, and stimulation of bilirubin conjugation .…”

“…Main Biotechnological Parameters of the A-18 Line a In contrast, calycosin(10), pseudobaptigenin(14), and derrone(15) seem to have a decreased ability to form conjugates because no glycosides of 14 and 15 were found, and only a small amount of the calycosin derivative 5 was identified (Table4). Maackiain(19) and maackiain-derived isoflavonoids were predominant in the calli (0.437% DW); other polyphenols ranged as follows (aglycone and its derivatives, % DW): formononetin (13), 0.348; genistein (12), 0.267; daidzein (9), 0.151; medicarpin (20), 0.131; calycosin (10), 0.038; derrone (15), 0.037; and pseudobaptigenin(14), 0.028.…”

Neuroprotective and Antiherpetic Properties of Polyphenolic Compounds from Maackia amurensis Heartwood

“…In addition to possessing hepatoprotective properties, Maksar ® also reduced the total lipid content in blood [ 4 , 5 ]. It prevented the increase in the total serum lipid content and the development of hyperlipoproteinemia in experimental animals [ 6 ] and in clinical trials [ 7 ].…”