Профиль Жирных Кислот Мембран Эритроцитов И Сыворотки Крови У Пациентов С Воспалительными Заболеваниями Кишечника В Зависимости От Стадии Заболевания

Кручинина, М. В.; Светлова, И. О.; Азгалдян, А. В.; Осипенко, М. Ф.; Валуйских, Екатерина Юрьевна; Шашков, М. В.; Соколова, А. С.; Кручинин, В. Н.; Яковина, И. Н.; Osipenko, Ivan

doi:10.37882/2223-2966.2021.05.18

Cited by 4 publications

(4 citation statements)

References 45 publications

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“…Предел обнаружения ЖК ~ 1 мкг на образец. Подробное описание пробоподготовки для исследования ЖК и процесса их определения представлено в работе [31]. Кроме содержания отдельных ЖК, определяли суммарное содержание насыщенных (НЖК), ненасыщенных (ННЖК), ПНЖК, омега-3 Эластичность печени, кПа 4,47 ± 0,64 5,51 ± 0,9 6,92 ± 0,88 6,57 ± 0,98 p1-3 = 0,028 p1-4 = 0,078…”

Section: материал и методыunclassified

“…Но вместе с тем имеются различия, связанные с основным этиологическим фактором ЖБП, которые могут быть полезны с точки зрения дифференциальной диагностики. В проведенных ранее исследованиях нами были получены пилотные данные об особенностях жирнокислотного профиля мембран эритроцитов и сыворотки крови у пациентов с ЖБП алкогольного и неалкогольного генеза по сравнению со здоровыми обследуемыми [19][20][21], определения тяжести ЖБП [22]. Полученные результаты оказались перспективными и требующими углубленных исследований с учетом роли жирных кислот в патогенезе ЖБП [23].…”

Section: Introductionunclassified

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Fatty Acids of Erythrocyte Membranes in the Differentiation of Patients with Fatty Liver Disease of Alcoholic, Non-Alcoholic and Mixed Genesis

Kruchinina,

Osipenko

et al. 2023

EPh

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The purpose of the study: to study the possibilities of using fatty acids of erythrocyte membranes in differentiating patients with fatty liver disease of alcoholic, non-alcoholic and mixed genesis (metabolic + alcohol). Material and methods. 38 men (average age 47.5 ± 2.9 years) with non-alcoholic fatty liver disease (NAFLD); 31 men with alcoholic fatty liver disease (AFLD) (45.1 ± 3.1 years) and 30 men (48.2 ± 3.2 years) with fatty liver disease of mixed genesis (metabolic + alcoholic) were examined. The degree of fibrosis in the groups did not exceed grade I. The composition and levels of the fatty acids (FA) of erythrocyte,s membranes were studied using gas chromatography/mass spectrometry, a system based on three Agilent 7000B quadrupoles (USA). Results. The levels of elaidic, 9-palmitoleic, eicosapentaenoic fatty acids were higher (p = 0.041–0.058), and the content of margaric, stearic and alpha-linolenic acids (p = 0.043–0.05) were lower in alcoholic genesis compared with non-alcoholic. The use of a panel of fatty acids – C18:1;t9, C17:0, C18:0, C18:3 n-3, C16:1;9, C20:5 n-3 – provided high diagnostic accuracy with an AUC of 0.914, sensitivity of 81%, specificity of 93% when distinguishing NAFLD from AFLD. It was found that the total content of unsaturated, monounsaturated, levels of arachidonic (n-6), oleic, eicosapentaenoic (n-3), palmitoleic, elaidic, the total content of two n-3 PUFA – eicosapentaenoic and docosahexaenoic in patients with mixed-genesis fatty liver disease were statistically significantly higher, and the levels of stearic, the total content of saturated, arachinic and the ratios of saturated FA/unsaturated FA, saturated FA/polyunsaturated FA were lower than in patients with non–alcoholic fatty liver disease (p = 0.000004–0.04). In patients with fatty liver disease of mixed genesis, the total content of monounsaturated FA, especially oleic and palmitoleic, linoleic (n-6) and saturated lauric, were significantly higher, and the levels of saturated FA arachinic, stearic, SFA/USFA, SFA/PUFA ratios were lower than in AFLD (p = 0.0009–0.06). Conclusion. The revealed differences in the levels of fatty acids of erythrocyte membranes in patients with fatty liver disease of various origins are probably associated with the main etiological factor and can be used to develop new diagnostic methods and targets for therapy.

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Section: материал и методыunclassified

Section: Introductionunclassified

Fatty Acids of Erythrocyte Membranes in the Differentiation of Patients with Fatty Liver Disease of Alcoholic, Non-Alcoholic and Mixed Genesis

Kruchinina,

Osipenko

et al. 2023

EPh

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“…Повышенный показатель электропроводности (p=0,004) и сниженный -емкости мембран клеток (p<0,001) у пациентов со стеатогепатитом свидетельствуют об утолщении мембран с повышением способности проводить электрический ток [12]. Подобные сдвиги могут быть обусловлены изменением соотношения в мембранах эритроцитов холестерина и фосфолипидов, уменьшением фракций легкоокисляемых фосфолипидов, изменением профиля жирных кислот (повышение уровней насыщенных, мононенасыщенных, линолевой, омега-6 полиненасыщенных жирных кислот (ПНЖК) и снижениеомега-3 ПНЖК, увеличение активности элонгазы (белка ELOVL6), показателя липогенеза de novo -C16:0/C18:2n-6 и снижение активности стеароил-КоА-десатуразы 1) [24] и связанной с этим нарушением активности вискозитропных трансмембранных ферментов [18].…”

Section: результаты и обсуждениеunclassified

Possibilities of using electrical and viscoelastic parameters of erythrocytes for the diagnosis of steatohepatitis in patients with fatty liver disease

Кручинина¹,

Паруликова²,

Белковец³

et al. 2023

Russian Medical Inquiry

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Aim: to study the possibilities of using electrical and viscoelastic parameters of erythrocytes to differentiate steatohepatitis from liver steatosis. Patients and Methods: 84 men (48.9±2.5 years) with fatty liver disease diagnosed by abdominal ultrasound were examined. Liver fibrosis severity, which did not exceed grade 1, was determined by indirect elastometry on the FibroScan® 502 Echosens device (France), with confirmation using the FibroTest–ActiTest test system (BioPredictive, France). Patients were divided into 2 groups according to the severity of hepatic necroinflammatory activity, assessed using the same test system. Group 1 (n=44) included patients with hepatic steatosis with minimal histologic disease activity (A 0–1); group 2 (n=40) included patients with steatohepatitis with significant necro-inflammatory activity (A 2–3). The electrical and viscoelastic parameters of erythrocytes were determined by dielectrophoresis at 4 frequencies of an inhomogeneous alternating electric field. Results and Discussion: the most significant electrical and viscoelastic indicators allowing to differentiate steatohepatitis from fatty liver disease were reduced values of strain amplitude at a frequency of 1 MHz (p=0.0003), dipole moment (p=0.009), capacity (p=0.014) and cell polarizability at a frequency of 1 MHz (p=0.03) and more high summarized viscosity (p=0.006), rigidity (p=0.005) and electrical conductivity (p=0.008). Diagnostic panel, including a set of electrical and viscoelastic parameters of erythrocytes, for the differentiation of steatohepatitis from steatosis demonstrated the following: AUC 0.904, sensitivity 0.9, specificity 0.83. Correlations of erythrocyte parameters with indicators of cytolysis syndrome (transaminase activity, serum iron levels, direct bilirubin) and serum inflammatory markers (fibrinogen, C-reactive protein, ferritin) were established. Conclusion: the electrical and viscoelastic parameters of erythrocytes studied using the dielectrophoresis should be considered as a new promising method in determining the severity of fatty liver disease. KEYWORDS: fatty liver disease, steatohepatitis, steatosis, electrical and viscoelastic parameters, erythrocytes, dielectrophoresis, diagnostic model. FOR CITATION: Kruchinina M.V., Parulikova M.V., Belkovets A.V., Gromov A.A. Possibilities of using electrical and viscoelastic parameters of erythrocytes for the diagnosis of steatohepatitis in patients with fatty liver disease. Russian Medical Inquiry. 2023;7(5):249–257 (in Russ.). DOI: 10.32364/2587-6821-2023-7-5-2.

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“…Кручининой и соавт. [11]. Кроме содержания отдельных ЖК определяли суммарное содержание насыщенных, ненасыщенных, полиненасыщенных, омега-3 ПНЖК, омега-6 ПНЖК, их соотношения.…”

Section: материалы и методыunclassified

Features of the fatty acid profile of erythrocyte membranes in patients with fatty liver disease of alcoholic genesis

Kruchinina,

Parulikova,

Belkovets

et al. 2023

Medicinskij sovet

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Introduction. Alcoholic steatosis, which is a reversible condition, is currently considered a significant risk factor for the progression of diffuse liver pathology, therefore understanding of its mechanisms at the molecular level is essential.Aim. To study the features of the fatty acid profile of erythrocyte membranes in patients with fatty liver disease of alcoholic origin for possible use of fatty acids (FAs) as biomarkers and potential therapeutic targets.Materials and methods. A total of 31 men with alcoholic fatty liver disease (AFLD) (average age of 45.1 ± 17.1 years) and 28 men of comparable age without AFLD and symptomatic pathology of internal organs were examined. The FA composition and levels of erythrocyte membranes (ER) were studied using Agilent 7000B (USA) triple quadrupole gas chromatography/mass spectrometry.Results and discussion. A higher level of a range of saturated FAs (lauric, margaric, pentadecane), monounsaturated FAs (MUFAs), which are additional factors for the progression of AFLD (palmitoleic, total monounsaturated acids), n-6/n-3 polyun-saturated FAs ratio (PUFAs), alpha-linolenic FA was detected in patients with AFL vs the control group (p = 0.00002–0.05). In contrast, the levels of arachidic and docosahexaenoic acids, total eicosapentaenoic and docosahexaenoic n-3 PUFAs, and total n-3 PUFAs were lower in patients with AFLD than in healthy men (p = 0.003–0.01), which is associated with increased ethanol induced adipose tissue lipolysis via PDE3B-AMPK axis. The use of FAs panel (C16:1;9, sum MUFA, n-6/n-3 PUFA, C22:6n3, C20:0) to distinguish patients with AFLD from healthy ones ensured high levels of sensitivity (79%), and specificity (81%) (AUC 0.808). Multidirectional associations of FA levels in erythrocyte membranes with each other and liver tests and lipid profile results were revealed.Conclusion. Thus, the features of erythrocytes membrane FAs in patients with AFLD and the potential to use them as biomarkers for differentiation of people with AFLD from healthy individuals have been identified.

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Профиль Жирных Кислот Мембран Эритроцитов И Сыворотки Крови У Пациентов С Воспалительными Заболеваниями Кишечника В Зависимости От Стадии Заболевания

Cited by 4 publications

References 45 publications

Fatty Acids of Erythrocyte Membranes in the Differentiation of Patients with Fatty Liver Disease of Alcoholic, Non-Alcoholic and Mixed Genesis

Fatty Acids of Erythrocyte Membranes in the Differentiation of Patients with Fatty Liver Disease of Alcoholic, Non-Alcoholic and Mixed Genesis

Possibilities of using electrical and viscoelastic parameters of erythrocytes for the diagnosis of steatohepatitis in patients with fatty liver disease

Features of the fatty acid profile of erythrocyte membranes in patients with fatty liver disease of alcoholic genesis

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